Effect of Alkaloids Isolated from <i>Phyllodium pulchellum</i> on Monoamine Levels and Monoamine Oxidase Activity in Rat Brain

Cai, Lu; Wang, Chao; Huo, Xiaokui; Dong, Peipei; Zhang, Baojing; Zhang, Hou-Li; Huang, Shanshan; Zhang, Bo; Yu, Sheng-ming; Zhong, Ming; Ma, Xiaochi

doi:10.1155/2016/6826175

Cited by 10 publications

(18 citation statements)

References 27 publications

(25 reference statements)

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“…(2) HPLC-FLD Analysis for DA and NE . DA and NE in brain tissue of the rats were detected rapidly and simultaneously by HPLC-FLD [ 34 ]. The instrument parameters used for HPLC-FLD were as follows: Agilent chromatographic system; Agilent ZORBAX C18 column (4.6 mm × 250 mm, 5 μ m); mobile phase: methanol-buffer (10 : 90); the buffer consisted of 0.1 mol/L NaAc and 0.1 mmol/L EDTA-2Na (pH 5.1 with hydrochloric acid); flow rate: 1.0 mL/min; and column temperature: 30°C.…”

Section: Methodsmentioning

confidence: 99%

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Effects and Components of Herb Pair Huanglian-Banxia on Diabetic Gastroparesis by Network Pharmacology

Liang

Zhang

Jiang

et al. 2021

BioMed Research International

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Diabetic gastroparesis (DGP) is a serious and chronic complication of long-standing diabetes mellitus, which brings a heavy burden to individuals and society. Traditional Chinese medicine (TCM) is considered a complementary and alternative therapy for DGP patients. Huanglian (Coptidis Rhizoma, HL) and Banxia (Pinelliae Rhizoma, BX) combined as herb pair have been frequently used in TCM prescriptions, which can effectively treat DGP in China. In this article, a practical application of TCM network pharmacological approach was used for the research on herb pair HL-BX in the treatment of DGP. Firstly, twenty-seven potential active components of HL-BX were screened from the TCMSP database, and their potential targets were also retrieved. Then, the compound-target network and PPI network were constructed from predicted common targets, and several key targets were found based on the degree of the network. Next, GO and KEGG enrichment analyses were conducted to obtain several significantly enriched terms. Finally, the experimental verification was made. The results demonstrated that network pharmacological approach was a powerful means for identifying bioactive ingredients and mechanisms of action for TCM. Network pharmacology provided an effective strategy for TCM modern research.

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Section: Methodsmentioning

confidence: 99%

“…The method of simultaneous detection of DA and NE was used according to the previous literature [34]. The contents of DA and NE were calculated from their respective standard curves.…”

Section: Effects Of Hl-bx On Da and Ne In Brain Tissue Of Dgpmentioning

confidence: 99%

Effects and Components of Herb Pair Huanglian-Banxia on Diabetic Gastroparesis by Network Pharmacology

Liang

Zhang

Jiang

et al. 2021

BioMed Research International

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“…The crude extract of P. pulchellum was found to possess antifibrotic [ 4 ], antioxidant [ 5 ], antitumor [ 6 ], antidiarrhea [ 3 ], antihypertensive [ 7 ], and antiarrhythmic [ 8 ] activities. Furthermore, the total alkaloids that were isolated from P. pulchellum also exhibited significant antifibrotic activity [ 9 , 10 , 11 ] and monoamine oxidase inhibitory activity [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Over the past five decades, a few chemical constituents were isolated and identified from P. pulchellum by various chromatographic, MS, and NMR technologies. There have been 18 alkaloids that have been reported, namely, N , N -dimethyltryptamine, gramine, 5-hydroxy- N , N -dimethyltryptamine, 5-hydroxy- N -methyltryptamine, 5-methoxy- N , N -dimethyltryptamine, 5-methoxy- N -methyltryptamine, N , N -dimethyltryptamine oxide, 5-methoxy- N , N -dimethyltryptamine-oxide, 5-hydroxy- N , N -dimethyltryptamine-oxide, N , N , N -trimethyltryptamine, 5-methoxy- N , N , N -trimethyl-1 H -indole-3-ethanaminium, 1-methyl-9 H -pyrido[3,4- b ]indol-2-ium, 6-methoxy-1,2-dimethyl-9H-pyrido[3,4- b ]indol-2-ium, 1,2-dimethyl-1,2,3,4-tetrahydro- β -carboline, 6-methoxy-2-methyl-1,2,3,4-tetrahydro- β -carboline, 3-indolcarbaldehyde, 3-indolcarbaldehyde, and uridine [ 7 , 12 , 13 , 14 , 15 ]. There have been 6 flavonoids, namely, pulcheloid B, citrusinol, yukovanol, 3,5,2′,4′-tetrahydroxy-2,2″-dimethylpyrano-[5″,6″,7,8]-flavanone, citflavanone, and 8-prenylated 5,7,3′,4′-tetrahydroxy flavanone [ 6 , 14 , 16 ], that have been reported.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Composition, Hepatoprotective, and Antioxidant Activities of Phyllodium pulchellum (L.) Desv

et al. 2018

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Phyllodiumpulchellum has been traditionally used as a medicinal herb because of its health-promoting effects, such as its hepatoprotective and antioxidant activities. In the present study, the petroleum ether fraction, ethyl acetate fraction, n-butanol fraction, and aqueous fraction were successively obtained from the ethanol extract of P. pulchellum. Two fractions, ethyl acetate fraction and n-butanol fraction, were found to display hepatoprotective and antioxidant activities. Further chemical investigation of the active fractions led to the isolation of its main constituents, including 11 flavonoids (1–11) and 8 indole alkaloids (12–19). There were 9 flavonoids (1–9) that were obtained from the ethyl acetate fraction, and 2 flavonoids (10 and 11) and 8 alkaloids (12–19) from the n-butanol fraction. Compounds 1–11 and 16–19 were isolated for the first time from P. pulchellum, and 1, 2, 8, 11, and 18 were obtained from the genus Phyllodium initially. Subsequently, the isolated compounds were evaluated for their in vitro hepatoprotective effects on the human normal hepatocyte cell line L-O2 injured by d-galactosamine and radical scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH). The flavonoids (−)-epigallocatechin (5) and (−)-epicatechin (6) exhibited prominent hepatoprotective activities with higher cell viability values (65.53% and 62.40% at 10 μM·mL−1, respectively) than the positive control, silymarin (61.85% at 10 μM·mL−1). In addition, compared with the positive control of vitamin C (IC50: 5.14 μg·mL−1), (−)-gallocatechin (3) and (−)-epigallocatechin (5) exhibited stronger antioxidant activities with IC50 values of 3.80 and 3.97 μg·mL−1, respectively. Furthermore, the total flavonoids from P. pulchellum were characterized using a high-performance liquid chromatography-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC-LTQ-Orbitrap-MS). In total, 34 flavonoids were tentatively identified, which had not been previously reported from P. pulchellum. In addition, we performed a semi-quantitative analysis of the isolated flavonoids. The contents of compounds 1–11 were 3.88, 17.73, 140.35, 41.93, 27.80, 4.34, 0.01, 0.20, 9.67, 795.85, and 5.23 μg·g−1, respectively. In conclusion, this study revealed that the flavonoids that were isolated from P. pulchellum showed hepatoprotective and antioxidant activities, indicating that, besides alkaloids, the flavonoids should be the potential pharmacodynamic ingredients that are responsible for the hepatoprotective and antioxidant activities of P. pulchellum.

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“…MAO is classified into two types (A and B), according to their sensitivity towards specificity substrates and inhibitors. [7] Different aminotransferases show different tissue distribution: aspartate aminotransferase activity is high across most tissues, whereas alanine aminotransferase activity is highest in the liver. [8] The alkaline phosphatase affects the inflammatory responses and may play a direct role in preventing organ damage, for example it affect on the inflammatory responses in patients with chronic kidney disease and is directly associated with erythropoiesis stimulating agent resistant anemia.…”

mentioning

confidence: 99%

Studying the Enzymes Levels in Cerebrospinal Fluid for Children With Acute Lymphoblastic Leukemia

Bdaiwi¹

2017

WJPPS

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The research includes studying of enzymes levels in the cerebrospinal fluid (CSF) for children with acute lymphoblastic leukemia (ALL) by determination of (9) parameter which include biochemical parameters: (73) were healthy children as control group which include (39) male and (34) female the age of children with acute lymphoblastic leukemia ranged from 1-15 year for both sex. The results showed there was a significant decrease in the activity of AChE, PChE and a significant increase in MAOA, AST, ALT, ALP, CK, LDH and SOD in CSF for children with acute lymphoblastic leukemia patients compared with control group for both sexes and these were a very good biochemical indicators for the patient body biochemical functions. The blood brain barrier loss its function in maintaining brain homoeostasis because of the oxidative stress and the age group (11-15)y of male had higher oxidative stress.

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Effect of Alkaloids Isolated from Phyllodium pulchellum on Monoamine Levels and Monoamine Oxidase Activity in Rat Brain

Cited by 10 publications

References 27 publications

Effects and Components of Herb Pair Huanglian-Banxia on Diabetic Gastroparesis by Network Pharmacology

Effects and Components of Herb Pair Huanglian-Banxia on Diabetic Gastroparesis by Network Pharmacology

Phytochemical Composition, Hepatoprotective, and Antioxidant Activities of Phyllodium pulchellum (L.) Desv

Studying the Enzymes Levels in Cerebrospinal Fluid for Children With Acute Lymphoblastic Leukemia

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