Effect of Alendronate on Cytoskeletal Organization and Dynamics of Actin in Pc-3 Prostate Cancer Cells

Virtanen, Sanna S.; Sandholm, Jouko; Väänänen, Kalervo; Härkönen, Pirkko

doi:10.1016/s1569-9056(06)61273-6

Cited by 2 publications

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“…They have a strong affinity for calcium phosphate, the major mineral component of bone, and hamper osteoclastic bone resorption by inhibiting the key enzyme farnesyl pyrophosphate synthetase 20,21. Recently, the therapeutic value of bisphosphonates has also been expanded by the discoveries that alendronate is effective in the prevention of urolithiasis by inhibition of calcium stone formation,22 as well as inhibiting invasion of PC-3 prostate cancer cells by affecting the mevalonate pathway 23. Therefore, evaluating the biocompatibility of calcium phosphate coated SiNWs (CaP/SiNWs) and bisphosphonate modified CaP/SiNWs composites is a valuable part of designing new effective orthopedic biomaterials if a permanent semiconducting conduit for bone regeneration is desired.…”

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confidence: 99%

Medicinal Surface Modification of Silicon Nanowires: Impact on Calcification and Stromal Cell Proliferation

Jiang

Fan

Belabassi

et al. 2008

ACS Appl. Mater. Interfaces

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Medicinal surface modification of silicon nanowires (SiNWs) with selected bisphosphonates, such as the known anti-osteoporotic drug alendronate, is described. In terms of specific assays relevant to orthopedic applications, the impact of selected bisphosphonates attachment on acellular calcification in simulated plasma is reported. To further investigate biocompatibility, proliferation assays of these modified nanowires were carried out using an orthopedically relevant cell line, mesenchymal stem cells derived from mouse stroma. It is found that the identity of the bisphosphonate ligand strongly and sensitively impacts their resultant cytotoxicity.

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confidence: 99%

Medicinal Surface Modification of Silicon Nanowires: Impact on Calcification and Stromal Cell Proliferation

Jiang

Fan

Belabassi

et al. 2008

ACS Appl. Mater. Interfaces

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“…25 Moreover, another bisphosphonate, alendronate, induced apoptosis and prevented invasion of prostate cancer cells by inhibiting the mevalonate pathway. 26 Finally, it was demonstrated that 10 µmol/L of zoledronate, which is also a new third generation of bisphosphonate, directly induced apoptosis in prostatic cancer cell lines. 27 These previous reports, taken together with our results, suggest that bisphosphonates directly induce prostate cancer cell apoptosis.…”

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confidence: 99%

Bisphosphonate induces apoptosis and inhibits pro‐osteoclastic gene expression in prostate cancer cells

et al. 2006

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Aim:Bisphosphonates are well established for the management of cancer-induced skeletal complications. Recent studies suggest that bisphosphonates promote apoptosis of cancer cells as well as osteoclasts in bone metastatic sites. To determine the direct effects of bisphosphonate on prostate cancer, we examined the effects of minodronate on prostatic cancer cell growth and the expression of apoptosis-related proteins and osteoclastogenic factors. Methods: PC-3, DU145 and LNCaP cells were treated with amino-bisphosphonate minodronate. Then proliferation, apoptosis and expression of bcl-2, bax, poly (ADP)-ribose polymerase (PARP), caspase-3, receptor activator of nuclear factor-κB ligand (RANKL), osteoprotegerin (OPG), matrix metalloproteinases-2 (MMP-2), and parathyroid hormone related protein (PTHrP) were assessed. Results: The proliferation of prostatic cancer cells was inhibited by minodronate. DNA fragmentation and TUNEL-positive nuclei were observed in minodronate-treated PC-3 cells. Minodronate decreased bcl-2 expression and induced bax expression, caspase-3 activity and degradation of PARP in DU145 and PC-3 cells. Minodronate decreased expression of RANKL, PTHrP and MMP-2 in PC-3 cells. Conclusions: Our results suggest that bisphosphonate not only promotes apoptosis directly but also decreases pro-osteoclastic gene expression in prostate cancer cells.

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Effect of Alendronate on Cytoskeletal Organization and Dynamics of Actin in Pc-3 Prostate Cancer Cells

Cited by 2 publications

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Medicinal Surface Modification of Silicon Nanowires: Impact on Calcification and Stromal Cell Proliferation

Medicinal Surface Modification of Silicon Nanowires: Impact on Calcification and Stromal Cell Proliferation

Bisphosphonate induces apoptosis and inhibits pro‐osteoclastic gene expression in prostate cancer cells

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