Effect of alcohols on the functional organization of the inner mitochondrial membrane

Lenaz, Giorgio; Parenti-Castelli, G; Monsigni, Novella; Silvestrini, Maria Grazia

doi:10.1007/bf01648927

Cited by 25 publications

(8 citation statements)

References 24 publications

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“…7) and the degree of inhibition was related to the mol wt of the alcohols (Table III). This agrees with other reports showing that the biological effectiveness of a series of n-alcohols was related to the chain length or mol wt of the alcohols (4,21,22,31,32). For example, the senescence retarding effect of alcohols in oat leaves increased in a log-linear fashion from ethanol to n-octanol, with a log increase in effectiveness related to a linear increase in mol wt of the alcohol (31).…”

Section: Effect Of Other Aliphatic Alcoholssupporting

confidence: 91%

“…In another study it was shown that ethanol disrupted the phospholipid bilayer immediately adjacent to the (Na+ + K+)-ATPase and caused its irreversible denaturation without significantly disrupting the protein structure of the membrane (10). The functional organization of the inner mitochondrial membrane was also disturbed by alcohols which disrupted lipid-protein bonds (22). Ethanol appears able to cause structural changes in cell membranes that result in increased permeability and denaturation of associated proteins.…”

Section: Resultsmentioning

confidence: 99%

“…Previous reports of the effect of alcohols on various biological processes have correlated the effectiveness of the alcohol with its lipophilia (22,31). Satler and Thimann (31) showed that the effectiveness of a series of aliphatic alcohols in delaying leaf senescence was paralleled by their ability to open stomata.…”

Section: Resultsmentioning

confidence: 99%

“…Another problem raised by a previous study was the reported inability of methanol to mimic ethanol inhibition of tomato fruit ripening (17). This observation proved to be inconsistent with other studies showing a progressive effect of a number of organic compounds on various metabolic functions with increasing mol wt (4,21,22,31).…”

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confidence: 78%

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Effect of Alcohols and Their Interaction with Ethylene on the Ripening of Epidermal Pericarp Discs of Tomato Fruit

Saltveit¹

1989

Plant Physiol.

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Ethanol concentrations that were induced in pericarp discs of mature-green tomato fruit (Lycopersicon esculentum Mill, cv ethylene. Methanol and n-propanol also inhibited lycopene synthesis without significantly increasing ion leakage. The similar inhibitory effects of methanol, ethanol, and n-propanol at concentrations which did not stimulate ion leakage, and the relationship between activity and lipophilia of the alcohols suggest that their mode of action was through disruption of membranes associated with ethylene action.

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Section: Effect Of Other Aliphatic Alcoholssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 78%

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Effect of Alcohols and Their Interaction with Ethylene on the Ripening of Epidermal Pericarp Discs of Tomato Fruit

Saltveit¹

1989

Plant Physiol.

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“…The ATPase of the mitochondrial inner membrane is also inhibited by aliphatic alcohols (9) and the effective alcohol concentrations similarly decrease in a log-linear fashion; but, in that work (9), all the concentrations were much higher than those active on senescence and, furthermore, at least up to C8, the carboxylic acids were more active than the alcohols. Grisham and Barnett (3) with a comparable (Na+ + K+)-ATPase, found the added CH2 group, but this was an irreversible inhibition of the enzyme, ascribed to denaturation.…”

Section: Discussionmentioning

confidence: 85%

The Influence of Aliphatic Alcohols on Leaf Senescence

Satler¹,

Thimann²

1980

Plant Physiol.

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Because of the effects of ethanol used as a solvent in other experiments, the action of aliphatic alcohols on leaf senescence in the dark has been studied systematically. These compounds both maintain chlorophyll and prevent proteolysis in the dark, much as do the cytokinins and other senescence-delaying substances. The activity of the straight-chain alcohols increases in a log-linear fashion with increasing chain length up to 1-octanol. Introduction of a branch in the chain or of a second OH group greatly decreases, or in some cases annuls, the antisenescence activity. In all cases, the action on senescence is closely (although not always exactly) paralleled by opening of the stomata. Abscisic acid and exposure to high concentrations of osmoticum, both of which close the stomata, antagonize the action of the alcohols. Some interactions with other agents are noted. The effects are compared with reported effects on seed germination, on hemolysis and animal membranes, and especially on permeability to K' ions, and a tentative basis for the mechanism of action is advanced.The ability of a number of chemically unrelated compounds to exert striking modifications of the progress of senescence in the detached leaves of oat seedlings has been reported elsewhere (22,24, and literature cited therein). Besides the cytokinins, at least five different compounds act to delay senescence in the dark, whereas three, as well as two physical treatments, promote the slower rate of senescence in the light, thus antagonizing the delaying effect of light. We found that in all cases the action on senescence was paralleled by an action on the stomatal aperture; stomatal closure paralleled promotion of senescence and stomatal opening paralleled its delay or inhibition.In studying these effects, it was necessary on several occasions to use alcohol as a solvent. Although the ethanol was diluted down to a maximum of 1% before application of the solution to the leaves, we noted that controls floated on this aqueous alcohol in darkness behaved very differently from those floated on H20. An example, with two concentrations of ethanol, is shown in Table I. Figures I and 2 of reference 24 also showed alcohol effects, but these were superimposed on the effects of FC2. It is clear from these data that ethanol exerts an effect on senescence in darkness like that of the several senescence inhibitors, although more weakly. Repeated observations of this alcohol effect led to a study of the action of a number of aliphatic alcohols on senescence. In this paper we report the considerable activity of a series of aliphatic alcohols in preventing or delaying leaf senescence in the dark. Disappearance of Chl and proteolysis responded similarly in all cases examined. The relation between structure and activity 'This work was supported in part by Grant BMS 7683126 from the National Science Foundation (to K. V. T.).2 Abbreviations: FC: fusicoccin; CCCP: carbonyl cyanide m-chlorophenylhydrazone. among the alcohols is closely parallel to reported effects...

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Lipid-Protein Interactions in the Structure of Biological Membranes

Lenaz

1972

Membrane Structure and Mechanisms of Biological Energy Transduction

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Effect of alcohols on the functional organization of the inner mitochondrial membrane

Cited by 25 publications

References 24 publications

Effect of Alcohols and Their Interaction with Ethylene on the Ripening of Epidermal Pericarp Discs of Tomato Fruit

Effect of Alcohols and Their Interaction with Ethylene on the Ripening of Epidermal Pericarp Discs of Tomato Fruit

The Influence of Aliphatic Alcohols on Leaf Senescence

Lipid-Protein Interactions in the Structure of Biological Membranes

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