Effect of Ahthocyanosides and Dexamethasone on Cultured Cells from Huilan Umbilical Vein

Abstract

The effects of Vaccinium myrtillue anthocyanosides, substances which demonstrated significant effect on the formation of new capillaries in experimental animals, and of dexamethasone were evaluated on cell cultures derived from umbilical vein. According to Shearn et al, pure endothelial cell suspensions were grown as primary and passed cell cultures. In the same way suspensions of highly contaminated specimens containing both ri broblasts and smooth muscle cells together with endothelial cells were cultured. Both types of culture were exposed to different concentrations of the two stimulants. In pure endothelial cell cultures, dexamethasone enhanced cell spreading as well as protein content. Anthocyanosides demonstrated a positive effect on cell proliferation and protein content. In different cell type cultures, dexamethasone stimulated the proliferation of endothelium more actively than other cells, while anthocyanoside s showed a stimulating activity on all different cellular types.