Effect of Agrobacterium rhizogenesis on hairy roots induction in fennel (Foeniculum vulgare Miller)

Abstract

This study was conducted to access the effects of seven Agrobacterium rhizogenes strains i.e. A4, T4, MSU, TCC15834, 2656, Gm1 and R1000, on the hairy root generation from various explants i.e. hypocotyl, cotyledon, root, stem and leaf of Fennel ecotypes (Bonab, Izmir, Esfahan and Moghan). Results of study revealed that induction of hairy root varies with the A. rhizogenes strains and used explants. Among the various tested combinations, highest root induction was recorded form hypocotyl explants using A4 and TCC 15834 (70 and 67%, respectively) strains of A. rhizogenes. Response of explant toward hairy root induction and number of the hairy roots per explant was different and decreased from hypocotyl, leaf and cotyledonary explants, respectively. Among the various tested A. rhizogenes strain, TCC 15834 capable to induced hairy Roots development in all ecotypes but most effectively in Izmir (36.66 ± 0.57) and it was followed by Bonab (32 ± 0.44), Esfahan (30 ± 0.99) and Moghan (16.66 ± 0.79). All transformed hairy root were successfully confirmed by PCR using rolB primers. Further hairy root induction could be proved as an alternative approach for the production of secondary metabolites and its increasing use as model system in metabolic engineering. KEYWORDS Fennel Agrobacterium rhizogenesHairy root culture Hairy root induction Explant type

Agrobacterium rhizogenes-mediated transformation of Hypericum sinaicum L. for the development of hairy roots containing hypericin

“…These results indicate that H. sinaicum plant organs may contain different compounds, or their contents, affecting the transformation process. Various responses of different plant tissues to A. rhizogenes were reported by (Abdi et al, 2017). Root and leaf segments of both H. tomentosum and H. tetrapterum species were used for transformation experiments with two A. rhizogenes wild-type, ATCC15834 and A4, agropine strains, but only the root segments were capable of transgenic hairy root formation (Komarovská et al, 2009).…”

“…They also show potential for stable production of various bioactive compounds that can be extracted if desired (Tusevski et al, 2013). Thus plant genetic engineering resulting from the transfer of Ri T-DNAs has been found as an effective indirect way of induction and high level accumulation of plant secondary metabolites using contained systems (Abdi et al, 2017). Induction of hairy roots using transgenesis approach is thus crucial, in particularly for medicinal plants as cultured hairy roots may be a convenient source of industrially important metabolites used in high quality medicinal products (Bafana, Lohiya, 2013).…”