Effect of Aging on Telomere Lengths in Bovine Oocytes and Granulosa Cells

Kin, Airi; Kansaku, Kazuki; Sumiya, Mei; Itami, Nobuhiko; Sirasuna, Koumei; Kuwayama, T.; Iwata, Hisataka

doi:10.1274/032.034.0101

Cited by 6 publications

(8 citation statements)

References 19 publications

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“…The bGCs in antral follicles of young cows showed a higher proliferation than aged cows [16]. Additionally, the reduction of telomere length is observed in bGCs of both early antral follicles and antral follicles from aged cows [17]. The lack of regenerative potential of bGCs was also observed in our in vitro study, as demonstrated by the reduced proliferation and the elevated cell cycle arrest in G0/G1 phase of bGCs in 14th passage.…”

Section: Discussionsupporting

confidence: 73%

Morphological changes during replicative senescence in bovine ovarian granulosa cells

et al. 2019

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The objective of this study was to evaluate replicative senescence of bovine granulosa cells (bGCs) during in vitro long-term culture. WST-1 assay analysis showed that bGCs proliferation was reduced from primary culture to 14th passage. The several bGCs from the 3rd passage and 7th passage exposed the weak activity of beta-galactosidase, while a strongly positive staining of beta-galactosidase was observed in bGCs from 14th passage. Flow cytometry analysis showed that bGCs were induced to cell cycle arrest at G0/G1 phase through in vitro expansion. TERT transcript expression of bGCs was downregulated from primary culture to 14th passage. The cell and nuclear area of bGCs were dramatically increased from 14th passage to 25th passage. The nucleocytoplasmic ratio of bGCs was dramatically reduced in 22th passage (4.32%) and 25th passage (2.45%), comparing to previous passages: primary culture (10.67%), 7th passage (9.21%), or 14th passage (10.33%). The number of microfilament bundle of bGCs was increased in 22nd passage (67.42 ± 17.76) and 25th passage (56.31 ± 22.45). The diameter of microfilament bundle of bGCs in 25th passage was dramatically increased to 1.88 ± 0.32 µm comparing to the primary culture (1.15 ± 0.03 µm). In this study, we also assessed the nuclear form factor which illustrates the level of nuclear circular form. A reduction of nuclear form factor was observed in bGCs during long-term in vitro expansion. The changes of nuclear form factor were correlated to other senescent characteristics, especially the nucleocytoplasmic ratio.

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Section: Discussionsupporting

confidence: 73%

Morphological changes during replicative senescence in bovine ovarian granulosa cells

et al. 2019

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“…The tendency towards a negative association between MAC and offspring TL (figure 2b) might be explained if oocytes that had fewer mitotic divisions tend to be ovulated earlier in the lifecycle than those ovulated later-although it is not clear if this occurs in humans [51]. In cows, it has been shown that TLs in immature oocytes are shorter in older cows than in younger ones, providing some support for this notion [52]. However, in cows TLs elongate during embryo development up until the blastocyst stage and TL has not been shown to differ between young and old cows at the blastocyst stage [52], suggesting that initial differences in oocyte TL may be obscured or eliminated through TL changes over the course of development.…”

Section: Is There a Maternal Age Effect On Tl In Humans?mentioning

confidence: 99%

The paternal age at conception effect on offspring telomere length: mechanistic, comparative and adaptive perspectives

Eisenberg

Kuzawa

2018

Phil. Trans. R. Soc. B

104

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Telomeres are repeating DNA found at the ends of chromosomes that, in the absence of restorative processes, shorten with cell replications and are implicated as a cause of senescence. It appears that sperm telomere length (TL) increases with age in humans, and as a result offspring of older fathers inherit longer telomeres. We review possible mechanisms underlying this paternal age at conception (PAC) effect on TL, including sperm telomere extension due to telomerase activity, age-dependent changes in the spermatogonial stem cell population (possibly driven by 'selfish' spermatogonia) and non-causal confounding. In contrast to the lengthening of TL with PAC, higher maternal age at conception appears to predict shorter offspring TL in humans. We review evidence for heterogeneity across species in the PAC effect on TL, which could relate to differences in statistical power, sperm production rates or testicular telomerase activity. Finally, we review the hypothesis that the PAC effect on TL may allow a gradual multi-generational adaptive calibration of maintenance effort, and reproductive lifespan, to local demographic conditions: descendants of males who reproduced at a later age are likely to find themselves in an environment where increased maintenance effort, allowing later reproduction, represents a fitness improving resource allocation.This article is part of the theme issue 'Understanding diversity in telomere dynamics'.

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“…Consistent with this, the levels of proliferation, as measured by the BrdU assay, was low in the granulosa cells of AFs from aged cows [ 59 ]. The telomere length of cells is closely linked to their proliferation activity, and an age-associated reduction in telomere length was observed in the granulosa cells of both the EAFs and AFs of cows [ 14 ]. The telomere length of granulosa cells or cumulus cells may represent a potential marker for oocyte ability in older women [ 60 ].…”

Section: Age-associated Changes In the Characteristics Of Granulosa Cmentioning

confidence: 99%

“…The telomere length of granulosa cells or cumulus cells may represent a potential marker for oocyte ability in older women [ 60 ]. A comparison of telomere length between oocytes and granulosa cells revealed significant positive correlations in the AFs of cows [ 14 ]. Telomeres shorten owing to the end replicable problem, and telomeres are vulnerable to DNA damage due to their GC-rich sequence [ 61 ].…”

Section: Age-associated Changes In the Characteristics Of Granulosa Cmentioning

confidence: 99%

“…In addition to developments in reproductive technologies, age-associated decline in the reproductive performance of cows is supported by evidences; for example, the number of embryos collected from aged cows (13–16 years old) following super-ovulation was lower than that collected from their younger daughters [ 10 ]. We have previously reported specific features of bovine oocytes that are associated with aging: premature progression of nuclear maturation [ 11 ], high abnormal fertilization rate following in vitro fertilization [ 12 ], low developmental ability [ 13 ], shorter telomere length [ 14 ], low lipid content in oocytes (unpublished data), and a low level of histone acetylation in germinal vesicle-stage oocytes [ 15 ]. In line with this, we conducted a comprehensive gene expression analysis of oocytes using next-generation sequencing technology (NGS) and compared between young (25–40 months) and aged (> 120 months) cows.…”

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confidence: 99%

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Age-associated changes in granulosa cells and follicular fluid in cows

Iwata

2017

Journal of Reproduction and Development

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Age-associated decline in oocyte quality is common in mammals. Oocytes take a long time to reach their full-grown size in large animals, and maternal physical conditions profoundly affect follicle development. Aging affects the oocyte itself as well as the surrounding environment, such as granulosa cells and follicular fluid. This review discusses age-associated changes that occur in granulosa cells and follicular fluid in cows and suggests that age-associated decline in granulosa cells and follicular fluid hampers proper oocyte development.

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Effect of Aging on Telomere Lengths in Bovine Oocytes and Granulosa Cells

Cited by 6 publications

References 19 publications

Morphological changes during replicative senescence in bovine ovarian granulosa cells

Morphological changes during replicative senescence in bovine ovarian granulosa cells

The paternal age at conception effect on offspring telomere length: mechanistic, comparative and adaptive perspectives

Age-associated changes in granulosa cells and follicular fluid in cows

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