Effect of aging and oral tolerance on dendritic cell function

Simioni, Patricia Ucelli; Fernandes, Luís Gustavo Romani; Gabriel, Dirce Lima; Tamashiro, Wirla Maria da Silva Cunha

doi:10.1590/s0100-879x2010000100010

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…Both Tesar et al and Jones et al 25 , 56 saw preserved TLR‐mediated modulation of co‐stimulatory molecules on aged DC. Studies using BM‐derived cDC and elicited peritoneal macrophages as APC have documented significant but limited reduction in the expression level of CD40, CD80 or CD86 with age 22 , 57 , 58 . Our and others’ data did not indicate any distinctive pattern of downregulation of these molecules with age.…”

Section: Discussioncontrasting

confidence: 50%

Phenotype and functions of conventional dendritic cells are not compromised in aged mice

et al. 2012

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Aging has profound effects on the immune system, including thymic involution, reduced diversity of the T cell receptor repertoire, reduced effector T cell and B cell function and chronic increase of proinflammatory cytokine production by innate immune cells. The precise effects of aging on conventional dendritic cells (cDC), the main antigen presenting cells of the immune system, however, are not well understood. We found that in aged mice the number of cDC in the spleen and lymph nodes remained stable, whereas the number of cDC in the lungs increased with age. Whereas cDC in mice showed similar cycling kinetics in all organs tested, cDC reconstitution by aged bone marrow precursors was relatively higher than that of their young counterparts. With the exception of CD86, young and aged cDC did not differ in their expression of co-stimulatory molecules at steady state. Most toll-like receptor (TLR) ligands induced comparable upregulation of co-stimulatory molecules CD40, CD86 and B7H1 on young and aged cDC, whereas TLR2 and TLR5 stimulation resulted in reduced upregulation of CD80 and CD86 on aged cDC in vitro. In vivo, influenza infection-induced upregulation of CD86, but not other co-stimulatory molecules, was lower in aged DC. Young and aged DC were equally capable of direct and cross presentation of antigens in vitro. Transcriptome analysis did not reveal any significant difference between young and aged cDC. These data show that unlike T and B cells, the maintenance of cDC throughout the life of a healthy animal is relatively robust during the aging process.

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Section: Discussioncontrasting

confidence: 50%

Phenotype and functions of conventional dendritic cells are not compromised in aged mice

et al. 2012

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“…Previous studies conducted in our laboratory showed that it is possible to induce oral tolerance to OVA in animals that exhibit a normal repertoire of receptors to the target antigen [31,57]. Several mechanisms seem to be involved in the induction of tolerance, including anergy, clonal deletion and induction of Treg cells [24,58–61].…”

Section: Resultsmentioning

confidence: 99%

Oral Tolerance Induced by OVA Intake Ameliorates TNBS-Induced Colitis in Mice

et al. 2017

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IntroductionLiterature data have shown that the consumption of dietary proteins may cause modulatory effects on the host immune system, process denominated oral tolerance by bystander suppression. It has been shown that the bystander suppression induced by dietary proteins can improve inflammatory diseases such as experimental arthritis. Here, we evaluated the effects of oral tolerance induced by ingestion of ovalbumin (OVA) on TNBS-induced colitis in mice, an experimental model for human Crohn’s disease.Methods and ResultsColitis was induced in BALB/c mice by instilling a single dose of TNBS (100 mg/kg) in ethanol into the colon. Tolerized mice received OVA (4mg/mL) dissolved in the drinking water for seven consecutive days, prior to or concomitantly with the intrarectal instillation. Control groups received protein-free water and ethanol by intrarectal route. We observed that either the prior or concomitant induction of oral tolerance were able to reduce the severity of colitis as noted by recovery of body weight gain, improvement of clinical signs and reduction of histological abnormalities. The in vitro proliferation of spleen cells from tolerant colitic mice was lower than that of control mice, the same as the frequencies of CD4+ T cells secreting IL-17 and IFN-γ. The frequencies of regulatory T cells and T cells secreting IL-10 have increased significantly in mice orally treated with OVA. The levels of inflammatory cytokines (IL-17A, TNF-α, IL-6 and IFN-γ) were lower in supernatants of cells from tolerant colitic mice, whereas IL-10 levels were higher.ConclusionOur data show that the modulation of immune response induced by oral tolerance reduces the severity of experimental colitis. Such modulation may be partially attributed to the increase of Treg cells and reduction of pro-inflammatory cytokines in peripheral lymphoid organs of tolerant mice by bystander suppression.

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Effect of aging and oral tolerance on dendritic cell function

Cited by 2 publications

References 26 publications

Phenotype and functions of conventional dendritic cells are not compromised in aged mice

Phenotype and functions of conventional dendritic cells are not compromised in aged mice

Oral Tolerance Induced by OVA Intake Ameliorates TNBS-Induced Colitis in Mice

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