Effect of age on the mercury sensitivity of zebrafish (Danio rerio) sperm

Pataki, Bernadett; Roberta, Berta Izabella; Gazsi, Gyöngyi; Urbányi, Béla; Kollár, Tímea; Horváth, Ákos

doi:10.1007/s10695-020-00875-9

Cited by 3 publications

(2 citation statements)

References 55 publications

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“…In contrast, the LIN, VAP, VSL, and VCL reflect the trajectory characteristics of the sperm. 34,48 In this study, no significant effects were observed for the velocity of the average pathway (VAP) and curvilinear velocity (VCL) of zebrafish sperm in all of the TBPH exposure groups (Figure 2G and H). In contrast, after 6 h of exposure, zebrafish sperm showed significant decreases of the VSL and VCL in all TBPH exposed groups; VAP in all treatments except for 0.1 μM; the progressive motility in 1 μM, 10 μM, and 60 μM TBPH treatments; and the total motility in 10 μM TBPH treatments.…”

Section: In Vitro and Ex Vivo Evidence For Disruptions On Male Reprod...mentioning

confidence: 59%

Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Fu,

Hua,

Zhang

et al. 2023

Environ. Sci. Technol.

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Bis(2-ethylhexyl)tetrabromophthalate (TBPH) has been widely detected in the environment and organisms; thus, its toxic effects on male reproduction were systematically studied. First, we found that TBPH can stably bind to the androgen receptor (AR) based on in silico molecular docking results and observed an antagonistic activity, but not agonistic activity, on the AR signaling pathway using a constructed AR-GRIP1 yeast assay. Subsequently, we validated the adverse effects on male germ cells by observing inhibited androgen production and proliferation in Leydig cells upon in vitro exposure and affected general motility and motive tracks of zebrafish sperm upon ex vivo exposure. Finally, the in vivo reproductive toxicity was demonstrated in male zebrafish by reduced mating behavior in F0 generation when paired with unexposed females and abnormal development of their offspring. In addition, reduced sperm motility and impaired germ cells in male zebrafish were also observed, which may be related to the disturbed homeostasis of sex hormones. Notably, the specifically suppressed AR in the brain provides further evidence for the antagonistic effects as above-mentioned. These results confirmed that TBPH affected male reproduction through a classical nuclear receptor-mediated pathway, which would be helpful for assessing the ecological and health risks of TBPH.

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Section: In Vitro and Ex Vivo Evidence For Disruptions On Male Reprod...mentioning

confidence: 59%

Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Fu,

Hua,

Zhang

et al. 2023

Environ. Sci. Technol.

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“…Dietrich et al [33] also reported decreased total motility below 50% and a significant reduction of VSL but not VCL in the semen of rainbow trout Oncorhynchus mykiss when they activated the sperm with inorganic-mercury-contaminated water (Hg 2+ , iHg) at 10,000 µg/L. Pataki et al [52] suggested that the total motility and VCL of zebrafish Danio rerio semen decreased when the sperm was activated with Hgcontaminated water between 500 and 5000 µg/L; however, they found that with older fish (7, 12, and 18 months) these parameters suffered more remarkable alterations. In the present study, the semen evaluated was extracted from bocachico breeders of the same age (24 months).…”

Section: Discussionmentioning

confidence: 98%

Effect of Inorganic Mercury on Semen Quality, Embryo and Larval Development of Bocachico Prochilodus magdalenae

Madariaga-Mendoza,

Marrugo-Negrete,

Atencio-García

2023

Fishes

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The study aimed to evaluate the effect of inorganic mercury (iHg) on the sperm quality, embryos, and larvae of Prochilodus magdalenae. Two experiments were carried out. In experiment 1, semen was activated with contaminated water at different iHg concentrations (0.0, 25, 50, 100 µg/L) and sperm kinetics were analyzed with a computer-assisted semen analysis (CASA) system. In the second trial, 2 g of oocytes were fertilized with 50 µL of milt, activated with contaminated water at different iHg concentrations (0.0, 25, 50, 100 µg/L), and maintained that way during the first hour of incubation. Samples were taken every 3–4 h until hatching to identify embryo deformations. Fertilization and hatching rates were estimated. The survival of newly hatched larvae (1 day) and larvae eight days post-hatching (dph) was also assessed. The results show that the quality parameters of semen treated with contaminated water at different iHg concentrations (25, 50, 100 µg/L) were altered and showed significant differences (p < 0.05) from the control group (0 µg/L). Total motility decreased (9.8–13.2%) and the amount of immotile sperm increased (3-fold) relative to values in the control group. A significant increase in oocyte diameter and a decrease in fertilization and hatching rates were observed with the higher iHg concentrations (50–100 µg/L). Embryo deformations (>40%) such as scoliosis, lordosis, and microcephaly were observed, as well as premature hatchings. The larval survival at 1 dph was reduced between 25% (25 µg/L) and 97.2% (100 µg/L) relative to that in the control group. The results suggest that bocachico semen, embryos, and larvae are sensitive to contamination with iHg from 25 µg/L.

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No general effects of advancing male age on ejaculates: a meta-analysis across the animal kingdom

Sanghvi

Vega‐Trejo

Nakagawa³

et al. 2023

Preprint

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Senescence, the deterioration of organismal function with advancing age, is a puzzling biological phenomenon. While actuarial senescence (i.e., age-dependent increases in mortality rates) is well described across some taxa, reproductive senescence (i.e.age- dependent declines in reproduction) is less understood, especially in males, with mixed patterns reported across studies. To examine the evidence for male reproductive senescence, we investigated how advancing male age affects ejaculate traits across non-human animals via a meta-analysis yielding 1814 effect sizes from 379 studies. We found no evidence for a general pattern of reproductive senescence. Instead, we found high heterogeneity for how reproduction changes with male age across animals. Some of this heterogeneity (>10%) was associated with biological factors. For example, there were taxonomical differences for some ejaculate traits — sperm motility declined with male age in lab rodents and fish, whereas ejaculate size improved with male age in bulls, fish, and insects. Some methodological factors were also important in explaining this heterogeneity: studies sampling a larger proportion of a species’ lifespan were more likely to detect senescence in ejaculate traits, emphasising the need to examine the full life cycle of species to document senescence. Contrary to predictions, we reveal that the evidence for senescence in ejaculate traits is sporadic. Our findings will help generate novel hypotheses and identify more effective methodological approaches for studying male reproductive senescence.

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Effect of age on the mercury sensitivity of zebrafish (Danio rerio) sperm

Cited by 3 publications

References 55 publications

Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Effect of Inorganic Mercury on Semen Quality, Embryo and Larval Development of Bocachico Prochilodus magdalenae

No general effects of advancing male age on ejaculates: a meta-analysis across the animal kingdom

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