Effect of Age on Adenosine Triphosphatase Activity from Tobacco Chloroplasts

Scherings, G.; Kuiper, P. J. C.

doi:10.1111/j.1399-3054.1974.tb03750.x

Cited by 4 publications

(5 citation statements)

References 8 publications

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“…Also, the activity cannot be attributed to the ATPase activity of the plastid envelope, since the envelope ATPase is mostly stimulated by Mg^+ (Cobb and Wellburn 1974), which rather showed an inhibitory effect in our system (Table 1, Figures 3 and 4). Our data support the reported inhibitory effect of Mg^+ on the activity of non-latent ATPase of tobacco chloroplasts (Scherings and Kuiper 1974). This would suggest that the membrane-bound ATPase (non-activated), in our system, is the enzyme of thylakoid membranes.…”

Section: Discussionsupporting

confidence: 92%

“…The activity of ATPase was measured by the method described by Scherings and Kuiper (1974). Chloroplasts were isolated in 0.4 M sucrose, 10 mM NaCl and 20 mM Tris-Cl buffer (pH 8.0).…”

Section: Assay Of a Tpasementioning

confidence: 99%

“…Only a few reports are available on aging-induced changes in the activities of adenosine triphosphatase (ATPase) (Scherings and Kuiper 1974) and ribulose diphosphate carboxylase (RuDPCase) (WoUgiehn 1967, Peterson andHufTaker 1975) of chloroplasts. Both ATPase and RuDPCase are complex enzyme systems weakly associated with the surface of plastid membranes (Sane 1977).…”

Section: Introductionmentioning

confidence: 99%

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Changes in the Ability of Photophosphorylation and Activities of Surface‐Bound Adenosine Triphosphatase and Ribulose Diphosphate Carboxylase of Chloroplasts Isolated from the Barley Leaves Senescing in Darkness

Biswal

Mohanty

1978

Physiologia Plantarum

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Dark induced aging of detached primary leaves of 11-day-old barley seedlings brings about a significant decline in the rates of ferricyanide |Fe(CN)jJ'" reduction and photophosphorylations of isolated chloroplasts. Ferricyanide-supported noncyclic photophosphorylation is somewhat more susceptible to leaf aging than phenazine methosulfate (PMS)-supported cyclic phosphorylation. Non-latent membrane-bound adenosine triphosphatase (ATPase) and ribulosediphosphate carboxylase (RuDPCase) lose about half of their initial activities after 24 h, while during this period the electron transport and photophosphorylation activities are much less affected. Also, the loss of RuDPCase is almost complete, while chloroplasts still exhibit a significant level of iFe(CN)^]'" reduction and photophosphorylations after 7 days of dark incubation. This would suggest that the enzymatic dark reactions are more sensitive to aging stress than the primary photochemical reactions of chloroplasts.Studies on the effect of divalent cations such as Mg^+ and Ca^+ on non-latent ATPase activity revealed that the dark stressed aging of detached leaves brings about a time dependent alteration in the response of this enzyme to Mg-'^, but not to Ca^+. The former showed inhibitory as well as stimulatory response, whereas the latter always caused the usual stimulation. Addition of kinetin (50 M) ensured retention of |Fe(CN)J^-reduction, photophosphorylations and RuDPCase activity in chloroplasts during leaf aging, but it failed to preserve the initial loss in the activity of the non-activated membrane-bound ATPase.

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Section: Discussionsupporting

confidence: 92%

“…The activity of ATPase was measured by the method described by Scherings and Kuiper (1974). Chloroplasts were isolated in 0.4 M sucrose, 10 mM NaCl and 20 mM Tris-Cl buffer (pH 8.0).…”

Section: Assay Of a Tpasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Changes in the Ability of Photophosphorylation and Activities of Surface‐Bound Adenosine Triphosphatase and Ribulose Diphosphate Carboxylase of Chloroplasts Isolated from the Barley Leaves Senescing in Darkness

Biswal

Mohanty

1978

Physiologia Plantarum

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“…4 and 5). The rate of ATP hydrolysis by tobacco chloroplasts also decreases with increasing leaf age (18). Chloroplasts isolated from older pea plants showed rates of ATP hydrolysis of 4 to 11 ,umol mg Chl-' hr-1 and 7 to 18 ,mol mg Chl-1 hr-1 for intact and shocked chloroplasts, respectively.…”

Section: Resultsmentioning

confidence: 91%

Stimulation of Carbon Dioxide Fixation in Isolated Pea Chloroplasts by Catalytic Amounts of Adenine Nucleotides

Robinson¹,

Wiskich²

1976

Plant Physiol.

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Carbon dioxide-dependent 02 evolution by isolated pea (Pisum sativum var. Massey Gem) chloroplasts was increased two to 12 times by the addition of ATP. 02 evolution was also stimulated by ADP and to a lesser extent by AMP. The ATP effects were not due to broken chloroplasts present in the preparations nor was ATP acting as a phosphate source. We concluded that the adenine nucleotides were acting catalytically. The concentration of ATP required for half-maximum rate of 02 evolution was 16 to 25 FAM. The degree to which ATP stimulated 02 evolution depended on the age of pea plants from which the chloroplasts were isolated. Spinach (Spinacia oleracea var. True Hybrid 102) chloroplasts did not show a consistent stimulation of 02 evolution by adenine nucleotides.The adenine nucleotide content of pea chloroplasts was not lower than that of spinach chloroplasts, but pea chloroplasts which showed a large stimulation of 02 evolution by ATP contained an ATP-hydrolyzing reaction with rates of 10 to 50 iLmol ATP hydrolyzed mg chlorophyll-' hour-'. The rate of the ATP-consuming reaction was much lower in spinach chloroplasts and in chloroplasts from older pea plants which did not show large stimulation of 02 evolution by ATP. We propose that the ATP-consuming reaction, with a high affinity for ATP, decreased the effective size of the ATP pool available for CO2 frixation. Added adenine nucleotides could be transported into the chloroplasts increasing the concentration of internal nucleotides. Calculations showed that the adenine nucleotide transporter on the outer chloroplast membranes could operate at a sufficient rate to produce such an effect. Adelaide 5001, Australia tion of 02 evolution by uncoupling agents. These reports suggest that adenine nucleotides penetrate intact chloroplasts to some extent. Jensen and Bassham (11) showed that ATP stimulated CO2 fixation to a small extent in intact chloroplasts, and that in the dark, ATP increased the level of ribulose-1,5-diphosphate. Studies of diffusion of compounds from isolated chloroplasts suggested that ATP and ADP diffuse out of chloroplasts but can re-enter and be used metabolically (2). Heldt (9) reported the presence of an adenine nucleotide transporter on the chloroplast outer membrane which apparently operates primarily to move ATP into the chloroplast, although the rates of transfer were low (5 ,umol mg Chl-' hr-'). The experiments of Heber and Santarius (8) similarly suggested low rates of adenine nucleotide transport into chloroplasts. Stokes and Walker (22) suggested that the failure of ATP to reverse uncoupler inhibition of P-glyceratedependent 02 evolution indicated impermeability of the chloroplast outer membrane to adenine nucleotides. It should be noted, however, that the adenine nucleotide transporter is inhibited by uncouplers (9), and that reversal of uncoupler inhibition requires the accumulation of substrate quantities of ATP.Stimulation of C02-dependent 02 evolution by ATP does not necessarily imply entry of ATP into chloroplasts. The ATP coul...

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“…Chloroplasts were isolated in a sucrose, tricine-HCl medium, pH 8.0 (Scherings and Kuiper 1974), except that the leaf discs or the leaves were homogenized in a Sorvall OtTinimixer.…”

Section: Isolation Of Chloroplastsmentioning

confidence: 99%

Glycolipid Degradation in Leaves of the Thermophilic Cucumis sativus as Affected by Light and Low‐Temperature Treatment

Kok

Kuiper

1977

Physiologia Plantarum

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Treatment of Cucumis leaf discs with light and low temperature (1°C) resulted in degradation of the total lipids. In addition, a decrease of the linolenic acid content of the glycolipids of leaves and leaf discs took place while at the same time an increase in the content of unidentified degradation products from the glycolipids was observed. The decrease of the linolenic acid content was not due to galactolipase activity, since no monogalactosyl diglyceride acyl hydrolase activity was found. Dark and low temperature did not alter the fatty acid composition. Blue light and low temperature resulted in a decrease of the linolenic acid content, while yellow‐, red‐ and far red light in combination with low temperature were ineffective.

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Effect of Age on Adenosine Triphosphatase Activity from Tobacco Chloroplasts

Cited by 4 publications

References 8 publications

Changes in the Ability of Photophosphorylation and Activities of Surface‐Bound Adenosine Triphosphatase and Ribulose Diphosphate Carboxylase of Chloroplasts Isolated from the Barley Leaves Senescing in Darkness

Changes in the Ability of Photophosphorylation and Activities of Surface‐Bound Adenosine Triphosphatase and Ribulose Diphosphate Carboxylase of Chloroplasts Isolated from the Barley Leaves Senescing in Darkness

Stimulation of Carbon Dioxide Fixation in Isolated Pea Chloroplasts by Catalytic Amounts of Adenine Nucleotides

Glycolipid Degradation in Leaves of the Thermophilic Cucumis sativus as Affected by Light and Low‐Temperature Treatment

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