Effect of Age of Boars on Testicular Lipids and Fatty Acids

La, Johnson; Vg, Pursel

doi:10.2527/jas1975.401108x

Cited by 6 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sperm-rich fraction of the ejaculates was collected using the gloved hand manual method. Pooled semen was extended in Beltsville Thawing solution (BTS; [31]) to 30×10 6 spermatozoa/ml (Inseminate doses). After collection, seminal characteristics (total sperm numbers per ejaculate, subjective sperm motility, acrosome integrity and normal morphology) were evaluated using standard laboratory techniques [32].…”

Section: Methodsmentioning

confidence: 99%

Early Developing Pig Embryos Mediate Their Own Environment in the Maternal Tract

et al. 2012

View full text Add to dashboard Cite

The maternal tract plays a critical role in the success of early embryonic development providing an optimal environment for establishment and maintenance of pregnancy. Preparation of this environment requires an intimate dialogue between the embryo and her mother. However, many intriguing aspects remain unknown in this unique communication system. To advance our understanding of the process by which a blastocyst is accepted by the endometrium and better address the clinical challenges of infertility and pregnancy failure, it is imperative to decipher this complex molecular dialogue. The objective of the present work is to define the local response of the maternal tract towards the embryo during the earliest stages of pregnancy. We used a novel in vivo experimental model that eliminated genetic variability and individual differences, followed by Affymetrix microarray to identify the signals involved in this embryo-maternal dialogue. Using laparoscopic insemination one oviduct of a sow was inseminated with spermatozoa and the contralateral oviduct was injected with diluent. This model allowed us to obtain samples from the oviduct and the tip of the uterine horn containing either embryos or oocytes from the same sow. Microarray analysis showed that most of the transcripts differentially expressed were down-regulated in the uterine horn in response to blastocysts when compared to oocytes. Many of the transcripts altered in response to the embryo in the uterine horn were related to the immune system. We used an in silico mathematical model to demonstrate the role of the embryo as a modulator of the immune system. This model revealed that relatively modest changes induced by the presence of the embryo could modulate the maternal immune response. These findings suggested that the presence of the embryo might regulate the immune system in the maternal tract to allow the refractory uterus to tolerate the embryo and support its development.

show abstract

Section: Methodsmentioning

confidence: 99%

Early Developing Pig Embryos Mediate Their Own Environment in the Maternal Tract

et al. 2012

View full text Add to dashboard Cite

show abstract

“…M aturation of testis during mammalian puberty increases the proportion of long‐chain highly polyunsaturated fatty acids (hPUFAs) within testicular phospholipids (PLs), as shown for rats (1), boars (2), bulls (3), monkeys (4), and humans (5). hPUFAs accumulate in phosphatidylcholine (PC) and phosphatidylethanolamine (PE) of developing monkey testis (4) and differentiating rat spermatocytes (6) and are strongly enriched in PC and PE of adult testis as compared with other tissues (2, 4, 7‐10) forming 8 to 24% of the fatty acids in human testis (11) and up to 58% in human semen (12). The highest levels of hPUFAs among testicular cell types (Leydig, Sertoli, and germ cells) were found for germ cells (13), which lack the enzymatic requirements for an efficient hPUFA biosynthesis and thus are dependent on their uptake (14–17).…”

mentioning

confidence: 98%

Polyunsaturated fatty acids are incorporated into maturating male mouse germ cells by lysophosphatidic acid acyltransferase 3

et al. 2011

View full text Add to dashboard Cite

Long-chain polyunsaturated fatty acids (PUFAs) accumulate in mammalian testis during puberty and are essential for fertility. To investigate whether lysophospholipid acyltransferases determine the PUFA composition of testicular phospholipids during pubertal development, we compared their mRNA expression, in vitro activity, and specificity with the lipidomic profile of major phospholipids. The accumulation of PUFAs in phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine correlated with an induced lysophosphatidic acid acyltransferase (LPAAT)3 mRNA expression, increased microsomal LPAAT3 activity, and shift of LPAAT specificity to PUFA-coenzyme A. LPAAT3 was induced during germ cell maturation, as shown by immunofluorescence microscopy. Accordingly, differentiation of mouse GC-2spd(ts) spermatocytes into spermatides up-regulated LPAAT3 mRNA, increased the amount of polyunsaturated phospholipids, and shifted the specificity for the incorporation of deuterium-labeled docosahexaenoic acid toward phosphatidylcholine and phosphatidylethanolamine. Stable knockdown of LPAAT3 in GC-2spd(ts) cells significantly decreased microsomal LPAAT3 activity, reduced levels of polyunsaturated phosphatidylethanolamine species, and impaired cell proliferation/survival during geneticin selection. We conclude that the induction of LPAAT3 during germ cell development critically contributes to the accumulation of PUFAs in testicular phospholipids, thereby possibly affecting sperm cell production.

show abstract

Linolenic acid deficiency

Tinoco

Babcock

Hincenbergs

et al. 1979

Lipids

126

View full text Add to dashboard Cite

Linolenic acid deficiency has not been demonstrated clearly in warm blooded animals, yet circumstantial evidence suggests that n-3 fatty acids may have functions in these animals. The fact that several species of fish definitely require dietary n-3 fatty acids indicates that n-3 fatty acids have important and specific functions in these animals and suggests that such functions may also be present in warm blooded animals. It is also true that n-3 fatty acid distribution in tissues of birds and mammals appears to be under strict metabolic control, and that this complex metabolic control mechanism apparently has survived evolutionary pressure for a very long time. So far, attempts to produce linolenic acid deficiency in mammals have not revealed an absolute requirement for n-3 fatty acids. If functions for n-3 fatty acids do exist in warm blooded animals, it seems probable that they may be located in the cerebral cortex or in the retina, because these tissues normally contain high concentrations of n-3 fatty acids.

show abstract

Effect of Age of Boars on Testicular Lipids and Fatty Acids

Cited by 6 publications

References 8 publications

Early Developing Pig Embryos Mediate Their Own Environment in the Maternal Tract

Early Developing Pig Embryos Mediate Their Own Environment in the Maternal Tract

Polyunsaturated fatty acids are incorporated into maturating male mouse germ cells by lysophosphatidic acid acyltransferase 3

Linolenic acid deficiency

Contact Info

Product

Resources

About