“…Sublethal effects may play a more important role than acute mortality, but there is a lack of studies to identify and quantify sublethal responses to pesticides in splittail. In addition, although several studies have demonstrated the acute and chronic toxicity of two common dormant spray insecticides, diazinon and esfenvalerate, in other fish species (Barry, Logan, Van Dam, Ahokas, & Holdway, 1995;Goodman, Hansen, Coppage, Moore, & Matthews, 1979;Holdway et al, 1994;Scholz et al, 2000;Tanner & Knuth, 1996), little work has been done integrating acute toxicity with biomarkers of exposure. Sublethal exposure to insecticides is expected to cause a wide range of responses (biomarkers) ranging from genetic to reproductive anomalies.…”