Effect of aerobic exercise on acquired gefitinib resistance in lung adenocarcinoma

Yang, Hong; Liu, Yiwen; Kong, Jinyu

doi:10.1016/j.tranon.2021.101204

Cited by 9 publications

(6 citation statements)

References 27 publications

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“…ESCC patients with Pg-positive infection have shorter survival and poor chemotherapy effect . In 2023, it was found that Pg could colonize the cytoplasm of ESCC cells, induce cancer cells to overexpress GSK3β, enhance their mitochondrial function, reduce their sensitivity to chemotherapy drugs, and promote their malignant evolution. , Therefore, it is essential to explore the molecular mechanism of Pg pathogenesis in ESCC for improving treatment and patient prognosis.…”

Section: Discussionmentioning

confidence: 99%

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Clinical Significance of Porphyromonas gingivalis Enriching Cancer Stem Cells by Inhibiting Programmed Cell Death Factor 4 in Esophageal Squamous Cell Carcinoma

Li,

Liu,

Zhou

et al. 2023

ACS Infect. Dis.

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Studies have confirmed that the colonization of Porphyromonas gingivalis (Pg) could promote the malignant evolution of esophageal squamous cell carcinoma (ESCC). Since pathogenic microorganisms can promote malignant tumor proliferation by inhibiting programmed cell death factor 4 (PDCD4) and the decrease of PDCD4 activity can enhance the stemness of cancer cells, we here investigated the functional mechanism by which Pg promoted ESCC chemoresistance and malignancy through inhibiting PDCD4 and enriching cancer stem cells (CSCs). The effects of Pg and PDCD4 on CSCs, chemoresistance and malignancy of ESCC cells were evaluated by in vitro studies. The expression of Pg, PDCD4, and ALDH1 in ESCC tissues were detected by IHC, and the correlations between each index and postoperative survival of ESCC patients were analyzed. The results showed that Pg could inhibit PDCD4 expression and lead to CSCs enrichment in ESCC cells. After eliminating Pg, the expression of PDCD4 was upregulated, the percentage of CSCs, chemoresistance and malignancy were decreased. ESCC patients with Pg-positive, PDCD4negative, and ALDH1-positive have a significant shorter survival. This study proved that eliminating Pg and blocking CSCs enrichment caused by decreasing PDCD4 activity may provide a new strategy for ESCC treatment.

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Section: Discussionmentioning

confidence: 99%

“…The percentage of ALDH1 + CSCs in each group was determined by Flow Cytometry (Beckman, USA). The difference in the percentage of ALDH1 + CSCs between the above two tubes was the final percentage of CSCs. , …”

Section: Methodsmentioning

confidence: 99%

Clinical Significance of Porphyromonas gingivalis Enriching Cancer Stem Cells by Inhibiting Programmed Cell Death Factor 4 in Esophageal Squamous Cell Carcinoma

Li,

Liu,

Zhou

et al. 2023

ACS Infect. Dis.

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“…After rewarming, secondary antibody (China Kangwei Biological Technology Co., 1:2000 TBST dilution) was added and incubated for 2 h. An ECL luminescent developer (Invitrogen Co. of America) was used to detect the target protein bands, a gel imaging system (BIO-RAD Co. of America) was used to collect images, and Image Lab software was used to measure the grey value of the protein bands. The grey value ratio between the target protein and the reference protein bands was used as the final relative protein expression [ 22–23 ]. The experiment was repeated three times.…”

Section: Methodsmentioning

confidence: 99%

Fusobacterium nucleatum induces MDSCs enrichment via activation the NLRP3 inflammosome in ESCC cells, leading to cisplatin resistance

et al. 2022

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Background To analyse the regulatory effect of Fusobacterium nucleatum (Fn) on NOD-like receptor protein 3 (NLRP3) and myeloid-derived suppressor cells (MDSCs) in oesophageal squamous cell carcinoma (ESCC) as well as its effect on cisplatin (CDDP) therapy and to explore its clinical significance. Methods Fn infection, NLRP3 expression and MDSCs infiltration in ESCC tissues were detected by RNAscope and immunohistochemistry (IHC). The correlation between these three factors and the clinicopathological features and survival of ESCC patients was analysed. A coculture system of human peripheral blood monocytes (PBMCs) and ESCC cells was established to simulate the tumour microenvironment. In vitro and in vivo models were used to analyse the effects of Fn on the percentage of MDSCs in the coculture system and the NLRP3 expression level and CDDP sensitivity of ESCC cells. Results Fn infection was consistent with high NLRP3 expression and MDSCs enrichment in ESCC tissues. Moreover, the survival time of ESCC patients was significantly shortened under Fn infection, high NLRP3 expression and MDSCs enrichment. In the in vitro and in vivo models, Fn induced abundant enrichment of MDSCs by inducing high expression of NLRP3 in ESCC cells and reducing the sensitivity of ESCC cells to CDDP. Conclusions Fn infection can induce high expression of NLRP3 in ESCC, lead to MDSCs enrichment, weaken the body's antitumour immunity, and lead to CDDP treatment resistance. The effective elimination of Fn and the inhibition of MDSCs enrichment may provide new strategies and treatments for ESCC. Highlights The survival of ESCC patients with Fn infection, high NLRP3 expression and MDSCs enrichment was significantly shortened. Fn infection could cause CDDP resistance in ESCC. Fn could induce the enrichment of MDSCs in the tumour microenvironment by activating NLRP3 in ESCC cells.

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“…Standard drugs for lung cancer treatment, cisplatin/gemcitabine,and menadione, reduced ALDH1 expression ( 68 ), while the elimination of ALDH1A1 significantly increased apoptosis and decreased resistance to cisplatin ( 69 ). Fat1 overexpression ( 70 ), aerobic exercise ( 71 ), nilotinib, erlotinib ( 72 ), all-trans retinoic acid ( 73 ), itraconazole ( 74 ), cryptotanshinone ( 55 ), CFTR31 ( 75 ), fluorescent interleukin ( 76 ), pomegranate ( 77 ), ginsenoside Rg3 ( 78 ), puerarin 6″-O-xyloside ( 79 ), glycolysis inhibitor PFK158 ( 67 ), globulin ( 80 ) can reduce expression of ALDH1 and drug resistance in lung cancer, mainly involved NF-κB pathway, Wnt pathway, Src-STAT3 signaling axis, Wnt/β-catenin/STAT3 axis, Akt/c-Myc signaling pathway. Therefore, in the treatment of lung cancer, targeting ALDH1 has broad prospects and should be further studied and explored.…”

Section: Aldh1 and Various Solid Tumorsmentioning

confidence: 99%

ALDH1: A potential therapeutic target for cancer stem cells in solid tumors

Wei

Chen

et al. 2022

Front. Oncol.

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Solid tumors can be divided into benign solid tumors and solid malignant tumors in the academic community, among which malignant solid tumors are called cancers. Cancer is the second leading cause of death in the world, and the global incidence of cancer is increasing yearly New cancer patients in China are always the first. After the concept of stem cells was introduced in the tumor community, the CSC markers represented by ALDH1 have been widely studied due to their strong CSC cell characteristics and potential to be the driving force of tumor metastasis. In the research results in the past five years, it has been found that ALDH1 is highly expressed in various solid cancers such as breast cancer, lung cancer, colorectal cancer, liver cancer, gastric cancer, cervical cancer, esophageal cancer, ovarian cancer, head,and neck cancer. ALDH1 can activate and transform various pathways (such as the USP28/MYC signaling pathway, ALDH1A1/HIF-1α/VEGF axis, wnt/β-catenin signaling pathway), as well as change the intracellular pH value to promote formation and maintenance, resulting in drug resistance in tumors. By targeting and inhibiting ALDH1 in tumor stem cells, it can enhance the sensitivity of drugs and inhibit the proliferation, differentiation, and metastasis of solid tumor stem cells to some extent. This review discusses the relationship and pathway of ALDH1 with various solid tumors. It proposes that ALDH1 may serve as a diagnosis and therapeutic target for CSC, providing new insights and new strategies for reliable tumor treatment.

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Effect of aerobic exercise on acquired gefitinib resistance in lung adenocarcinoma

Cited by 9 publications

References 27 publications

Clinical Significance of Porphyromonas gingivalis Enriching Cancer Stem Cells by Inhibiting Programmed Cell Death Factor 4 in Esophageal Squamous Cell Carcinoma

Clinical Significance of Porphyromonas gingivalis Enriching Cancer Stem Cells by Inhibiting Programmed Cell Death Factor 4 in Esophageal Squamous Cell Carcinoma

Fusobacterium nucleatum induces MDSCs enrichment via activation the NLRP3 inflammosome in ESCC cells, leading to cisplatin resistance

ALDH1: A potential therapeutic target for cancer stem cells in solid tumors

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