Effect of advanced glycation end products on platelet activation and aggregation: a comparative study of the role of glyoxal and methylglyoxal

Arriagada-Petersen, Cristian; Fernandez, Paula; Gomez, Maira; Ravello, Natalia; Palomo, Iván; Fuentes, Eduardo; Ávila, Felipe

doi:10.1080/09537104.2020.1767770

Cited by 6 publications

(2 citation statements)

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“…Among many cell types that express RAGE receptors are platelets [17]. A large body of research has established that, under pathological circumstances, RAGE plays a significant role in platelet hyperactivation and thrombus formation [18,19]. Vascular problems in patients with coronary artery disease (CAD) and diabetes mellitus (DM) have been investigated extensively, and the results reveal a correlation between RAGE gene polymorphism and both conditions [20].…”

Section: Introductionmentioning

confidence: 99%

Untitled

2023

J. Med. Chem. Sci.

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Background: Transmembrane multiligand receptor RAGE is a protein found on the surface of cells that binds to the advanced glycation end products. Platelet cyclooxygenase activity is elevated in response to RAGE activation, contributing to the formation of a thrombus. Objectives: To examine the connection between RAGE gene rs80096349(C>T), rs1035798(C>T), and rs184003(G>T) polymorphisms and resistance to aspirin in CAD patients from Iraq. Patients and methods: Patients with coronary artery disease (CAD) (161 males and 64 females) were enrolled in the trial between February 2021 and October 2021. All participants were taking prophylactic Aspirin (100 mg). The control group consisted of 130 individuals, including 97 males and 33 females, who appeared to be in good health and were not taking aspirin. Serum thromboxane B2 levels were used to assess the effectiveness of aspirin (TBX2). Patients were classified as either aspirin sensitive or resistant. The polymorphism of RAGE's most closely related single nucleotide polymorphisms (SNPs) was identified using polymerase chain reaction for amplification of the extracted deoxyribonucleic acid and Sanger's method of sequencing. Results: A total of 225 cardiovascular patients compatible with inclusion criteria were enrolled. There were 161(71.6%) male and 64(28.4%) female patients with a mean age of 56.85±8.11years old. The statistical analysis revealed 17.8% (n = 40) aspirin-resistant cases in the study population. Genotypic analysis of our data showed that participants with the T allele of rs184003(G/T) had an increased prevalence in the aspirin-resistant group compared with the aspirin-sensitive group (p<0.05). In contrast, for rs10835798, the frequency was dramatically higher for T alleles in aspirin sensitive group contrasted with the resistant group (p<0.05). The aspirinresistant group was found to have significantly greater RAGE levels than the sensitive group. Conclusion: Our results provide evidence that RAGE expression and gene rs1035798(C>T), and rs184003(G>T) polymorphisms could be predictors for CAD patients' resistance to aspirin.

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Section: Introductionmentioning

confidence: 99%

Untitled

2023

J. Med. Chem. Sci.

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“…Thus, an increase in MG production via glycolysis coupled with impaired degradation from reduced expression of Glo1 will lead to accumulation of MG in blood and tissues of COVID-19 patients. At supraphysiologic levels, MG will disrupt the function of vascular endothelial cells resulting in microvascular leakage, clot and tissue brosis [38][39][40][41] . Supraphysiological levels of MG will also potentiate in ammation and oxidative stress by activating NF-κB and the NLR family pyrin domain containing 3 (NRLP3) in ammasome, and the activities of complex I and III of the electron transport chain in mitochondria 42,43 .…”

Section: Introductionmentioning

confidence: 99%

Elevated Plasma Level of the Glycolysis Byproduct Methylglyoxal on Admission Is an Independent Biomarker of Mortality in ICU COVID-19 Patients

Alomar

Alshakhs

Abohelaika

et al. 2021

Preprint

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Biomarkers to identify ICU COVID-19 patients at high risk for mortality are urgently needed for therapeutic care and management. In this study we found that plasma levels of the glycolysis byproduct methylglyoxal (MG) were 4.4-fold and 1.7-fold higher (P<0.0001) upon ICU admission in patients that later died (n=34) compared to uninfected controls (n=30) and those survived (n=31), respectively. The increase in MG was inversely correlated with glutathione (r2=-0.63) and the MG-glutathione degrading glyoxalase-1 (r2=-0.50), and positively correlated with the inflammation markers, SSAO (r2 =0.52), TNF-a (r2 =0.41), IL-1b (r2 =0.25), CRP (r2 =0.26) and age (r2 =0.20). Logistic regression analysis provides evidence of a significant relationship between the elevated MG upon admission into ICU and death (P<0.0001), with 42% of the death variability explained. From these data we conclude that elevated plasma MG on admission is a novel independent biomarker that predicts ICU COVID-19 patients at high risk of mortality.

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