Effect of adrenergic and Ca2+ antagonists on increased ornithine decarboxylase expression in regenerating rat liver

Desiderio, Maria Alfonsina; Lugaro, G; Galasso, Daniela; Colombo, Mario P.

doi:10.1016/0006-2952(90)90462-t

Cited by 12 publications

(5 citation statements)

References 44 publications

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“…The relative amount of gas-6 mRNA decreased below the level of control liver by 4 hours after PH, returned to basal values at 8 hours, and then increased again, reaching a maximum at 16 hours; 24 hours after PH gas-6 expression returned to the uninduced level. In agreement with previous results (Desiderio et al, 1990), ODC mRNA was induced by 4 hours with a peak at 8 hours of liver regeneration, whereas the level of GADPH mRNA, which is known to remain constant throughout the cell cycle (Manfioletti et al, 19901, was approximately the same in all samples. Hybridization of RNA from livers of sham-operated rats (Fig.…”

Section: Levels Of Gas-6 Mrna During Liver Regenerationsupporting

confidence: 92%

“…The rats of the first group were partially hepatectomized by the method of Higgins and Anderson (1931). Some of them, at the time of the surgical operation, were treated with prazosin (1 mgiKg body weight, intraperitoneally (i.p.1, Desiderio et al, 1990), Actinomycin D (0.4 mg/Kg body weight, i.p., Desiderio et al, 1982), or trifluoperazine (30 mg/Kg body weight, i.p., Desiderio et al, 1990); neomycin (100 mg/Kg body weight, subcutaneously (s.c.)) was injected daily for 6 days before the operation (Desiderio et al, 1990). The hepatectomized rats were killed at various times after the operation.…”

Section: Animals and Treatmentsmentioning

confidence: 99%

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Expression of a growth arrest specific gene (gas‐6) during liver regeneration: Molecular mechanisms and signalling pathways

Ferrero¹,

Desiderio²,

Martinotti³

et al. 1994

Journal Cellular Physiology

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A set of growth arrest-specific (gas) genes negatively regulated by serum has been identified. To define the role of gas genes in a model of cell proliferation in vivo we analyzed the expression of one of these genes (gas-6) during liver regeneration after partial hepatectomy (PH). We found that gas-6 mRNA was down-regulated 4 hours after PH, within the G0 to G1 transition. Later on, gas-6 mRNA increased over the level found in normal liver with a peak at 16 hours, before the onset of DNA synthesis. This surge was probably triggered by an inflammatory response caused by the surgical trauma, because an increase of similar extent occurring with the same time course was present in livers of sham-operated and turpentine-treated rats. Comparison of mRNA steady state levels with nuclear transcription rates indicated that gas-6 expression is post-transcriptionally regulated. As we found that down-regulation of gas-6 expression was prevented by treatment with Actinomycin D, a labile protein might be involved in the determination of gas-6 mRNA stability. To investigate the mitogenic signals controlling gas-6 expression during liver regeneration we treated hepatectomized rats with a specific alpha-1-adrenoceptor blocker (prazosin) as well as with drugs which modify intracellular calcium levels. The decrease of gas-6 mRNA 4 hours after PH was prevented by prazosin and by neomycin, an inhibitor of calcium release from endogenous stores. These findings suggest that down-regulation of gas-6 expression during hepatic regeneration is triggered by catecholamines interaction with alpha-1-adrenergic receptors and by subsequent calcium release. In addition we found that the rise of gas-6 gene expression occurring at 16 hours after PH was not affected by prazosin but was inhibited by trifluoperazine. Therefore, we suggest that up-regulation of gas-6 gene expression is mediated by the interaction of calcium with calmodulin, independently of catecholamines.

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Section: Levels Of Gas-6 Mrna During Liver Regenerationsupporting

confidence: 92%

Section: Animals and Treatmentsmentioning

confidence: 99%

Expression of a growth arrest specific gene (gas‐6) during liver regeneration: Molecular mechanisms and signalling pathways

Ferrero¹,

Desiderio²,

Martinotti³

et al. 1994

Journal Cellular Physiology

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“…In addition to the polyamines and their analogues are there other factors that influence antizyme synthesis? Many studies have reported an association between cellular calcium levels and polyamine synthesis and transport [16][17][18][19][20][21]. We hypothesized that the noted effects could be explained, at least partially, if alterations in calcium levels affected the activity of the regulator of polyamine transport and synthesis, antizyme.…”

Section: Antizyme Synthesis Requires Calciummentioning

confidence: 96%

Antizyme modifications affecting polyamine homoeostasis

Mitchell

Judd

1998

Biochemical Society Transactions

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“…Northern-blot analysis and molecular hybridization Preparation of total RNA and Northern-blot analysis were performed as previously described [14][15][16].…”

Section: Experimental Materialsmentioning

confidence: 99%

“…The reaction mixture for ODC (final volume 500,u) contained the cytosolic supernatant (about 400 ,g of protein), 50 mM Tris/HCl (pH 7.1) and 1 mM ['4C]ornithine (sp. radioactivity 1 mCi/mmol) [16,24]. The reaction mixture for AdoMetDC (final volume 125 ,1) contained the cytosolic supernatant (about 200,g of protein), 2.5 mM putrescine, 0.4 mM labelled Sadenosylmethionine (sp.…”

Section: Odc and Adometdc Activlty Assaysmentioning

confidence: 99%

Cytosolic and nuclear spermidine acetyltransferases in growing NIH 3T3 fibroblasts stimulated with serum or polyamines: relationship to polyamine-biosynthetic decarboxylases and histone acetyltransferase

Desiderio

Mattei²,

Biondi³

et al. 1993

Biochemical Journal

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The expression (mRNA level of enzymic activity) of cytosolic and nuclear spermidine acetyltransferases was studied in NIH 3T3 fibroblasts, either (1) serum-starved and stimulated to grow by serum refeeding, or (2) treated with inhibitors of ornithine decarboxylase (ODC) (MDL 72.175) and S-adenosylmethionine decarboxylase (AdoMetDC) (MDL 73.811) and stimulated to grow by spermidine. Expression of the known growth-regulated genes for ODC, AdoMetDC and histone acetyltransferase was also examined. The mRNA for spermidine/spermine N1-acetyltransferase (SAT) accumulated after serum refeeding (between 6 and 16 h) and even more after spermidine addition (16 h). Histone acetyltransferase activity increased after both growth stimuli, whereas spermidine N8-acetyltransferase activity remained unchanged. After serum stimulation, the ODC mRNA level and activity rose between 6 and 16 h, whereas AdoMetDC mRNA accumulation occurred later (16 h) than the increase in enzyme activity (6 h). Stimulation of ODC and AdoMetDC activities was suppressed by the inhibitors added alone or in combination with spermidine, whereas mRNA accumulation was down-regulated by spermidine. These results indicate that the expression of SAT was growth-controlled and that SAT mRNA level was regulated by polyamines.

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Effect of adrenergic and Ca2+ antagonists on increased ornithine decarboxylase expression in regenerating rat liver

Cited by 12 publications

References 44 publications

Expression of a growth arrest specific gene (gas‐6) during liver regeneration: Molecular mechanisms and signalling pathways

Expression of a growth arrest specific gene (gas‐6) during liver regeneration: Molecular mechanisms and signalling pathways

Antizyme modifications affecting polyamine homoeostasis

Cytosolic and nuclear spermidine acetyltransferases in growing NIH 3T3 fibroblasts stimulated with serum or polyamines: relationship to polyamine-biosynthetic decarboxylases and histone acetyltransferase

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