Effect of adipose-derived nucleated cell fractions on tendon repair in horses with collagenase-induced tendinitis

Abstract

ADNC injection improved tendon organization in treated tendons. Although biochemical and molecular differences were less profound, tendons appeared architecturally improved after ADNC injection, which was corroborated by improved tendon COMP expression. Use of ADNC in horses with tendinitis appears warranted.

Mesenchymal stem cells and insulin‐like growth factor‐I gene‐enhanced mesenchymal stem cells improve structural aspects of healing in equine flexor digitorum superficialis tendons

“…6,7,40 The collagenase-induced model of tendinitis is well documented and has been used extensively in equine and lab animal studies as a model for human tendinitis. 20,22,25,[41][42][43][44][45] The use of this technique to create a suitable model for the study of naturally occurring tendinitis has been supported by the evaluation of gross and histopathological changes, clinical signs, and ultrasonographic findings following the induction of injury. 44,[46][47][48] In addition, our laboratory has successfully investigated the effects of recombinant IGF-I on equine tendon healing using this collagenase induced model of tendinitis.…”

“…A total of 2000 units of collagenase were delivered to two sites centered in the tensile region of the SDFT tendon under ultrasonographic guidance, as described by this group previously. 20,22 The horses were confined to box stalls and their forelimbs bandaged. Each horse served as its own control, with one forelimb randomly assigned as the treated limb and the other as the control.…”

“…RNA was assessed by spectrophotometry at 260:280 nm and by 1% agarose gel electrophoresis. RNA was used for quantitative RT/PCR of collagen types I and III, 22,24 IGF-I, 25 and cartilage oligomeric matrix protein (COMP), [26][27][28] as indicators of tendon matrix synthesis, and matrix metalloproteinase-3 (MMP-3), [29][30][31] matrix metalloproteinase-13 (MMP-13), 24,29,32 and aggrecanase (ADAMTS-4) 30,33 as indicators of tendon catabolism.…”

“…All tendon parameters were scored from l (normal) to 4 (severe changes) for: tenocyte shape, tenocyte density, free hemorrhage, neovascularization, perivascular cuffing, collagen fiber linearity, collagen fiber uniformity, polarized light crimping, and collagen type I deposition as previously described. 22 Scores from both observers were averaged. This grading scheme expands on previously described systems which utilize a four-parameter score.…”

Expression of scleraxis and tenascin C in equine adipose and umbilical cord blood derived stem cells is dependent upon substrata and FGF supplementation

“…AdMSC proliferate more rapidly than UCB regardless of the surface substrate (Reed and Johnson 2012), suggesting a difference in regenerative capabilities as more plastic stem cells often have longer population doubling times than more differentiated cells (Stojkovic et al 2004). Adipose derived mononuclear cells were capable of improving tendon architecture but not biomechanical properties in a collagenase induced lesion of the SDFT (Nixon et al 2008). Transplantation of bone marrow derived stem cells into tendon lesions results in decreased lesion size and greater tendon density (Crovace et al 2007;Pacini et al 2007).…”