Effect of adipose-derived mesenchymal stromal cells on tendon healing in aging and estrogen deficiency: an in vitro co-culture model

Veronesi, Francesca; Bella, Elena Della; Torricelli, Paola; Pagani, Stefania; Fini, Milena

doi:10.1016/j.jcyt.2015.07.007

Cited by 19 publications

(13 citation statements)

References 40 publications

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“…In this trial, we used autologous AD MSCs with proven safety . Besides easiness of harvesting tissues in a large quantity with minimal invasiveness, one of the major strengths of AD MSCs is that they are less influenced by age or morbidity of patients in contrast to bone marrow MSCs or tendon stem/progenitor cells . In addition, several experimental studies showed that AD MSCs prevented progression of tendon degeneration , increased the tensile strength and type I collagen in tendon repair , decreased fatty infiltration of muscle after repair , decreased tendon defects in human lateral epicondylosis , and reduced the re‐injury rate in race horses .…”

Section: Discussionmentioning

confidence: 99%

Intratendinous Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Rotator Cuff Disease: A First-In-Human Trial

et al. 2018

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Despite relatively good results of current symptomatic treatments for rotator cuff disease, there has been an unmet need for fundamental treatments to halt or reverse the progress of disease. The purpose of this study was to assess the safety and efficacy of intratendinous injection of autologous adipose tissue-derived mesenchymal stem cells (AD MSCs) in patients with rotator cuff disease. The first part of the study consists of three dose-escalation cohorts; the low- (1.0 × 10 cells), mid- (5.0 × 10 ), and high-dose (1.0 × 10 ) groups with three patients each for the evaluation of the safety and tolerability. The second part included nine patients receiving the high-dose for the evaluation of the exploratory efficacy. The primary outcomes were the safety and the shoulder pain and disability index (SPADI). Secondary outcomes included clinical, radiological, and arthroscopic evaluations. Twenty patients were enrolled in the study, and two patients were excluded. Intratendinous injection of AD MSCs was not associated with adverse events. It significantly decreased the SPADI scores by 80% and 77% in the mid- and high-dose groups, respectively. Shoulder pain was significantly alleviated by 71% in the high-dose group. Magnetic resonance imaging examination showed that volume of the bursal-side defect significantly decreased by 90% in the high-dose group. Arthroscopic examination demonstrated that volume of the articular- and bursal-side defects decreased by 83% and 90% in the mid- and high-dose groups, respectively. Intratendinous injection of autologous AD MSCs in patient with a partial-thickness rotator cuff tear did not cause adverse events, but improved shoulder function, and relieved pain through regeneration of rotator cuff tendon. Stem Cells 2018;36:1441-1450.

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Section: Discussionmentioning

confidence: 99%

Intratendinous Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Rotator Cuff Disease: A First-In-Human Trial

et al. 2018

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“…40, 31 Previous studies have suggested that local mesenchymal stromal cell populations may exhibit a varying predilection for proteoglycan expression, and this variation has been thought to explain differences in glycosaminoglycan staining post-injury. 22, 41 It is also possible that a combination of mechanical loading environment 42 and time post-injury may drive GAG accumulation, but this remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Temporal Healing of Achilles Tendons After Injury in Rodents Depends on Surgical Treatment and Activity

Freedman

Salka²,

Morris³

et al. 2017

J Am Acad Orthop Surg

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Introduction Achilles tendon ruptures affect 15 (women) to 55 (men) per 100,000 people each year, and controversy continues to exist regarding optimal treatment and rehabilitation protocols. The objective of this study was to investigate the temporal effects of surgical repair and immobilization/activity (IM/ACT) on Achilles healing and limb function following complete transection in rodents. Methods Injured tendons (n=128) were repaired or left non-repaired, and animals groups immobilized in plantarflexion for 1, 3, or 6 weeks that later resumed cage and treadmill activity for 5, 3, or 0 weeks (IM1/ACT5, IM3/ACT3, IM6/ACT0). Animals were euthanized after 1- or 6-weeks post-injury. Results At 6-weeks post injury, IM1/ACT5 groups had increased range of motion and decreased ankle joint toe stiffness compared to IM3/ACT3 groups. IM6/ACT0 had decreased tendon cross sectional area, but increased tendon echogenicity and collagen alignment. Surgical treatment dramatically decreased fatigue cycles to failure in repaired tendons from earlier IM1/ACT5 groups. Normalized comparisons between 6- and 1-week post-injury data demonstrated that changes in healing tendon properties (area, alignment, and echogenicity) were maximized by IM1/ACT5 compared to IM6/ACT0. Discussion/Conclusion This study demonstrates how the temporal post-injury healing response of rodent Achilles tendons depends on both surgical treatment and the timing of IM/ACT.

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“…However, the fact that subcutaneous adipose tissue is a relatively accessible reservoir for adult stem cell harvest and that ADSCs are less influenced by age or morbidity of patients in contrast of BMCs, thus working in favor of the clinical use of ADSCs. 36 Moreover, bone marrow aspirate concentrate has shown to have less stem cells per unit volume than ADSCs. 37 In addition, ADSCs do not provoke a strong lymphocytic reaction but they instead release immunosuppressive factors that enable the allogeneic and autologous ADSCs to engraft successfully for musculoskeletal tissue regeneration purposes.…”

Section: Adscs: Harvesting Preparation and Cellular Characteristicsmentioning

confidence: 99%

The role of adipose derived stem cells in the treatment of rotator cuff tears: from basic science to clinical application

Mocini¹,

Monteleone²,

Piazza³

et al. 2020

Orthop Rev (Pavia)

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Over the last decade, regenerative medicine has become increasingly popular throughout the scientific community. The poor healing capacity at the tendon-bone interface makes the rotator cuff an appealing target for biologic agents. Adipose derived stem cells are mesenchymal cells with the capacity for self-renewal and multipotential differentiation. They have been recently proposed, both in isolation and as adjuvants to existing surgical therapies, for the treatment of rotator cuff tears. Several studies have been carried out in this research field, starting from the biological characteristics of adipose derived stem cells, their preparation and culture, up to the application in the experimental field on animal models and on humans. The purpose of this study was to provide a state of the art about the current basic science and clinical literature for the effectiveness of adipose derived stem cells in the treatment of rotator cuff tears.

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Effect of adipose-derived mesenchymal stromal cells on tendon healing in aging and estrogen deficiency: an in vitro co-culture model

Cited by 19 publications

References 40 publications

Intratendinous Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Rotator Cuff Disease: A First-In-Human Trial

Intratendinous Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Rotator Cuff Disease: A First-In-Human Trial

Temporal Healing of Achilles Tendons After Injury in Rodents Depends on Surgical Treatment and Activity

The role of adipose derived stem cells in the treatment of rotator cuff tears: from basic science to clinical application

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