Effect of adiponectin on bovine granulosa cell steroidogenesis, oocyte maturation and embryo development

Abstract

BackgroundAdiponectin is an adipokine, mainly produced by adipose tissue. It regulates several reproductive processes. The protein expression of the adiponectin system (adiponectin, its receptors, AdipoR1 and AdipoR2 and the APPL1 adaptor) in bovine ovary and its role on ovarian cells and embryo, remain however to be determined.MethodsHere, we identified the adiponectin system in bovine ovarian cells and embryo using RT-PCR, immunoblotting and immunohistochemistry. Furthermore, we investigated in vitro the effects of recombinant human adiponectin (10 micro g/mL) on proliferation of granulosa cells (GC) measured by [3H] thymidine incorporation, progesterone and estradiol secretions measured by radioimmunoassay in the culture medium of GC, nuclear oocyte maturation and early embryo development.ResultsWe show that the mRNAs and proteins for the adiponectin system are present in bovine ovary (small and large follicles and corpus luteum) and embryo. Adiponectin, AdipoR1 and AdipoR2 were more precisely localized in oocyte, GC and theca cells. Adiponectin increased IGF-1 10(-8) M-induced GC proliferation (P < 0.01) but not basal or insulin 10(-8) M-induced proliferation. Additionally, adiponectin decreased insulin 10(-8) M-induced, but not basal or IGF-1 10(-8) M-induced secretions of progesterone (P < 0.01) and estradiol (P < 0.05) by GC. This decrease in insulin-induced steroidogenesis was associated with a decrease in ERK1/2 MAPK phosphorylation in GC pre-treated with adiponectin. Finally, addition of adiponectin during in vitro maturation affected neither the percentage of oocyte in metaphase-II nor 48-h cleavage and blastocyst day 8 rates.ConclusionsIn bovine species, adiponectin decreased insulin-induced steroidogenesis and increased IGF-1-induced proliferation of cultured GC through a potential involvement of ERK1/2 MAPK pathway, whereas it did not modify oocyte maturation and embryo development in vitro.

The effect of nutrition and metabolic status on the development of follicles, oocytes and embryos in ruminants

“…Indeed, the expression of adiponectin, AdipoR1 and AdipoR2 was higher in the theca and cumulus cells and the oocytes of dominant follicles compared with those of atretic follicles during both the follicular and luteal phases (Tabandeh et al, 2012). In our laboratory, we have shown that adiponectin decreased insulin-induced steroidogenesis and increased IGF-I-induced proliferation of the bovine granulosa cells in culture possibly by a mechanism that involved the ERK1/2 MAPK pathway (Maillard et al, 2010). Lagaly et al (2008), have also observed an inhibitory effect of adiponectin on bovine steroidogenesis but only in the theca cells.…”

“…In the bovine species, adiponectin and its receptors are present in different follicular cells (oocytes, theca, granulosa and cumulus cells) and luteal cells (Maillard et al, 2010) (Table 1). Moreover, it has been shown that the physiological status of the ovary is related to the pattern of expression of adiponectin and its receptors in follicular and luteal cells from the bovine ovary (Tabandeh et al, 2010).…”

The influence of adiponectin on the transcriptomic profile of porcine luteal cells

“…Adiponectin had an inhibitory effect on in vitro secretion of progesterone by porcine luteal cells on days 10 to 12 of the estrous cycle (Maleszka et al 2014a). The effect of adiponectin on ovarian basal progesterone secretion was also suggested in studies on cows, rats, and humans (Chabrolle et al 2009;Mailard et al 2010;Lagaly et al 2008). Moreover, the insulin-induced production of progesterone and estradiol by bovine granulosa cells was significantly decreased as a result of adiponectin treatment (Mailard et al 2010).…”

“…The effect of adiponectin on ovarian basal progesterone secretion was also suggested in studies on cows, rats, and humans (Chabrolle et al 2009;Mailard et al 2010;Lagaly et al 2008). Moreover, the insulin-induced production of progesterone and estradiol by bovine granulosa cells was significantly decreased as a result of adiponectin treatment (Mailard et al 2010). In the study with bovine theca interna cells, Lagaly et al (2008) observed that adiponectin decreased LH and insulin-induced production of progesterone.…”

“…Recent studies suggest that adiponectin may be a hormonal link between them. The presence of adiponectin and its receptors was found at all levels of the hypothalamicpituitary-gonadal (HPG) axis in the pig, human, rat, cow, and chicken (Ledoux et al 2006;Chabrolle et al 2007aChabrolle et al , 2007bChabrolle et al , 2009Mailard et al 2010).…”