Effect of adenosine deaminase inhibition upon human lymphocyte blastogenesis.

Carson, Dennis A.; Seegmiller, J. Edwin

doi:10.1172/jci108278

Cited by 176 publications

(40 citation statements)

References 23 publications

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“…Where indicated, the medium was supplemented with 5 μM deoxynucleosides along with a medium change. To prevent degradation of individual deoxynucleosides, 0.5 μM immucillin (37) was added together with GdR and 10 μM erythro-9-2-hydroxy-3-nonyladenine (38) with AdR.…”

Section: Methodsmentioning

confidence: 99%

Mammalian ribonucleotide reductase subunit p53R2 is required for mitochondrial DNA replication and DNA repair in quiescent cells

Pontarin

Ferraro

Bee

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

114

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In postmitotic mammalian cells, protein p53R2 substitutes for protein R2 as a subunit of ribonucleotide reductase. In human patients with mutations in RRM2B, the gene for p53R2, mitochondrial (mt) DNA synthesis is defective, and skeletal muscle presents severe mtDNA depletion. Skin fibroblasts isolated from a patient with a lethal homozygous missense mutation of p53R2 grow normally in culture with an unchanged complement of mtDNA. During active growth, the four dNTP pools do not differ in size from normal controls, whereas during quiescence, the dCTP and dGTP pools decrease to 50% of the control. We investigate the ability of these mutated fibroblasts to synthesize mtDNA and repair DNA after exposure to UV irradiation. Ethidium bromide depleted both mutant and normal cells of mtDNA. On withdrawal of the drug, mtDNA recovered equally well in cycling mutant and control cells, whereas during quiescence, the mutant fibroblasts remained deficient. Addition of deoxynucleosides to the medium increased intracellular dNTP pools and normalized mtDNA synthesis. Quiescent mutant fibroblasts were also deficient in the repair of UV-induced DNA damage, as indicated by delayed recovery of dsDNA analyzed by fluorometric analysis of DNA unwinding and the more extensive and prolonged phosphorylation of histone H2AX after irradiation. Supplementation by deoxynucleosides improved DNA repair. Our results show that in nontransformed cells only during quiescence, protein p53R2 is required for maintenance of mtDNA and for optimal DNA repair after UV damage.DNA precursors | dNTP de novo synthesis | cell cycle | mitochondrial disease D NA replication and repair require the continued synthesis of the four dNTPs. They are synthesized by evolutionary-related ribonucleotide reductases operating with slightly different mechanisms in aerobic and anaerobic organisms (1). Each ribonucleotide reductase provides the required amounts of all four dNTPs. A similar allosteric mechanism, maintained throughout evolution, regulates both the enzyme's activity and its substrate specificity. Cells contain small dNTP pools of similar sizes, approximately 10-fold larger during DNA replication than during quiescence. Regulation of pool sizes by ribonucleotide reductases is of great importance for correct DNA replication, and changes in the actual sizes or in their balance lead to increased mutation rates (2). For mammalian cells, the induction of mutations by pool imbalances has been described in detail, along with possible mechanisms (3). In yeast, a recent elegant study (4) linked specific amino acid substitutions in the catalytic subunit of ribonucleotide reductase to defined pool imbalances, which result in increased mutation rates.In mammalian cells, the canonical ribonucleotide reductase is a complex between two proteins: the large catalytic protein R1 that contains the allosteric sites and the smaller protein R2 that contributes a stable tyrosyl free radical during the reaction (1). Both proteins are transcriptionally activated during early S-phase (5) a...

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Section: Methodsmentioning

confidence: 99%

Mammalian ribonucleotide reductase subunit p53R2 is required for mitochondrial DNA replication and DNA repair in quiescent cells

Pontarin

Ferraro

Bee

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

114

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“…This enzyme activity is accelerated in response to mitogenic and immunogenic inducers . Also being an enzyme its effect can be blocked by specific inhibitors [8,9]. The ADA activity lags in disorders of cell mediated immunity as in AIDS.…”

Section: Observations and Resultsmentioning

confidence: 99%

A Prospective Study to comparatively assess the role of adenosine deaminase levels in contents of cold and pyogenic abscesses

Rampal¹,

Prajapati²,

Sharma³

2016

IJIMS

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“…Cell culture models and animal experiments indicated that lymphocytes and lymphoid cells could grow normally when their ADA activity was almost completely inhibited by EHNA or coformycin (Carson and Seegmiller 1976;Ullman et al . 1976;Burridge et al 1977).…”

Section: Discussionmentioning

confidence: 99%

A lymphoblastoid cell line from an adenosine deaminase deficient patient established by epstein-barr virus.

Tsuchiya

Narisawa

Konno

et al. 1979

Tohoku J. Exp. Med.

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Effect of adenosine deaminase inhibition upon human lymphocyte blastogenesis.

Cited by 176 publications

References 23 publications

Mammalian ribonucleotide reductase subunit p53R2 is required for mitochondrial DNA replication and DNA repair in quiescent cells

Mammalian ribonucleotide reductase subunit p53R2 is required for mitochondrial DNA replication and DNA repair in quiescent cells

A Prospective Study to comparatively assess the role of adenosine deaminase levels in contents of cold and pyogenic abscesses

A lymphoblastoid cell line from an adenosine deaminase deficient patient established by epstein-barr virus.

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