Effect of Additives on the Activity of Tannase from Aspergillus awamori MTCC9299

Chhokar, Vinod; Sangwan, M. S.; Beniwal, Vikas; Nehra, Kiran; Nehra, Kaur Singh

doi:10.1007/s12010-009-8813-7

Cited by 43 publications

(30 citation statements)

References 18 publications

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“…tannases with an optimum activity at a pH of 5.5. Tannases from Aspergillus awamori and Aspergillus heteromorphus also are optimally active at pH 5.5 [32,33]. A. awamori nakazawa exhibited optimum activity at pH 5.0 [34], and tannase from Penicillium variable was found to be stable at pH range of 3.0-8.0 [35].…”

Section: Discussionmentioning

confidence: 98%

Catalytic and Thermodynamic Properties of a Tannase Produced by Aspergillus niger GH1 Grown on Polyurethane Foam

Ramos

Mata-Gómez

Rodríguez‐Durán

et al. 2011

Appl Biochem Biotechnol

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Tannase is an inducible enzyme with important applications in the food and pharmaceutical industries. This enzyme was produced by the fungus Aspergillus niger GH1 under solid-state fermentation using polyurethane foam as solid support and tannic acid as sole carbon source and tannase inducer. Physicochemical properties of A. niger tannase were characterized, and the kinetic and thermodynamics parameters on methyl gallate hydrolysis were evaluated. The enzyme was stable in a pH range of 2-8 and a functional temperature range of 25-65 °C. The highest k(cat) value was 2,611.10 s(-1) at 65 °C. Tannase had more affinity for methyl gallate at 45 °C with a K(M) value of 1.82 mM and an efficiency of hydrolysis (k(cat)/K(M)) of 330.01 s(-1) mM(-1). The lowest E(a) value was found to be 21.38 kJ/mol at 4.4 mM of methyl gallate. The lowest free energy of Gibbs (ΔG) and enthalpy (ΔH) were found to be 64.86 and 18.56 kJ/mol, respectively. Entropy (ΔS) was -0.22 kJ/mol K. Results suggest that the A. niger GH1 tannase is an attractive enzyme for industrial applications due its catalytic and thermodynamical properties.

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Section: Discussionmentioning

confidence: 98%

Catalytic and Thermodynamic Properties of a Tannase Produced by Aspergillus niger GH1 Grown on Polyurethane Foam

Ramos

Mata-Gómez

Rodríguez‐Durán

et al. 2011

Appl Biochem Biotechnol

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“…The synthesis of the enzyme was reduced in SR medium maintained under agitation (16.31 U/mL). Czapeck medium is one of the most cited for the production of tannase by fungi, such as A. awamori MTCC9299 [29], A. niger GH1 [30] and A. oryzae [25]. Adams medium was more favorable for the enzyme production by A. japonicus 246A (19.8 U/mg of protein) and Aspergillus sp.…”

Section: Effect Of Culture Mediummentioning

confidence: 99%

Screening, Selection and Optimization of the Culture Conditions for Tannase Production by Endophytic Fungi Isolated from Caatinga

Cavalcanti¹,

Ornela²,

Jorge³

et al. 2017

J App Biol Biotech

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Tannin acyl hydrolase (EC 3.1.1.20), called tannase, is an enzyme of great biotechnological interest for applications in food, chemical, beverage and pharmaceutical industries. Therefore, the objective of this study was to isolate, select and identify strains of endophytic fungi from rich tannin plants collected in the Caatinga, as well as, the optimization of culture conditions for the best producers. Sixteen endophyte fungi were isolated from the barks of mastic (Myracrodruon urundeuva Allemão), angico (Anadenanthera colubrina Vell.), barauna (Schinopsis brasiliensis Engl.), cajueiro (Anacardium occidentale L.) and catingueira (Caesalpinia pyramidalis Tul). All strains showed ability of using tannic acid as carbon source. The species A. niger and A. fumigatus isolated from angico and cajueiro, respectively, presented the highest enzyme production. The optimum conditions for the production of tannase by A. niger were 24 h cultivation in Khanna medium containing 2% tannic acid, in the absence of nitrogen source, at 37 °C. A. fumigatus showed increased production of tannase when cultured in mineral medium for 24 h using 2% tannic acid as carbon source and peptone as additional nitrogen source, at 37 ºC. The optimum apparent temperature and pH of activity for the enzymes produced by both fungal species were 30 ºC and 4.0, respectively.

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“…Chhokar et al (2010) reported that maximum production of tannase was achieved after 48 h of fermentation using Aspergillus heteromorphus MTCC 8818. However, observed maximum production only after 98 h using Penicillium atramentosum.…”

Section: Resultsmentioning

confidence: 99%

“…Chhokar et al (2010) reports a yield of 19.29% when purified derived from 1% concentration of tannic acid while using A. heteromorphus MTCC 8818. Raaman et al (2010) used a tannic acid concentration of 1.5% (89 U/mL) along with Paecilomyces variotii.…”

Section: Resultsmentioning

confidence: 99%

Tannase production by Aspergillus spp. UCP1284 using cashew bagasse under solid state fermentation

Liu¹,

Moreira²,

Campos-Takaki³

et al. 2016

Afr. J. Microbiol. Res.

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Production of tannase by Aspergillus species UCP1284 was studied using solid state fermentation and cashew bagasse as substrate. Amount of 35 strains of fungi species isolated from soil of Caatinga were used for qualitative selection of strains with potential for production of tannase. Through the selected fungi, a complex study was achieved about the influence of the variables: substrate amount, initial moisture content and tannic acid concentration on the production of tannase by solid state fermentation, using a factorial design (2 3 ). The maximum activity was 12.26 U/g of dry substrate obtained at the time of 48 hours using 10.0 g of substrate with initial moisture content of 40% and using 2.0% of tannic acid. This first design for enzyme production demonstrated the influence of the studied parameters, mainly, substrate amount on the enzyme yield, and its relevance in the process for the subsequent optimization protocol.

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Effect of Additives on the Activity of Tannase from Aspergillus awamori MTCC9299

Cited by 43 publications

References 18 publications

Catalytic and Thermodynamic Properties of a Tannase Produced by Aspergillus niger GH1 Grown on Polyurethane Foam

Catalytic and Thermodynamic Properties of a Tannase Produced by Aspergillus niger GH1 Grown on Polyurethane Foam

Screening, Selection and Optimization of the Culture Conditions for Tannase Production by Endophytic Fungi Isolated from Caatinga

Tannase production by Aspergillus spp. UCP1284 using cashew bagasse under solid state fermentation

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