Effect of additives on the esterification activity of immobilized Candida antarctica lipase

Abstract

In this work, the stabilizing effect of bovine serum albumin (BSA), peptone (PEP), and polyethylene glycol (PEG) during immobilization of Candida antarctica lipase on activated carbon was investigated. The influence of enzyme concentration and type of additive, added during the immobilization procedure, was studied using a 2 2 factorial central composite design. The goal was to maximize the synthetic activity of butyl butyrate, using butyric acid and butanol as substrate in n-heptane. An increase of 31-58% in the esterification activity was obtained when enzyme concentration on the supernatant was enhanced from 86.50 U m L -1 to 226.80 U mL -1 . An enhancement in esterification activity of 38-68.95% was observed, depending on the initial enzyme concentration, when PEP was used instead of BSA. No significant increase in the esterification activity was observed when PEP was replaced by PEG. However, thermal stability tests at 50°C showed that PEG had a higher stabilizing effect.

Formulation and characterization of an immobilized laccase biocatalyst and its application to eliminate organic micropollutants in wastewater

“…Previous studies have reported that the biocatalyst activity can be significantly enhanced if immobilization is performed in the presence of macromolecular additives such as proteins (albumin, gelatin, casein) and polymers (polyethylene glycol, polyvinyl alcohol). It was suggested that the additive can prevent the loss of enzyme activity by enhancing the cross-linking of the enzyme and by providing a stable molecular conformation to the enzyme [25,26]. Our results however showed that the addition of BSA had a detrimental effect on the resulting biocatalyst activity.…”

Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles

“…[28] Therefore, those interactions should not be affected by changes in the pH adsorption. On the other hand, if electrostatic forces are important, changes over the isoelectric point of lipase will have a large impact on the binding constants.…”

Application of home-made lipase in the production of geranyl propionate by esterification of geraniol and propionic acid in solvent-free system

“…The synthesis of citronellol laurate in organic media catalyzed by immobilized lipases was investigated by Yadav and Lathi (2004)observed a decrease between 62% and 55% in Novozym 435 activity after 3 cycles of reuse and conversions lower than 20% at this moment. Rodrigues et al (2008), investigating the synthesis of butyl butyrate using CALB lipase immobilized in soluble activated carbon, found that after 6 cycles, only 10-15% of the initial activity was retained. Guncheva et al (2011) immobilized Candida rugosa lipase and the biocatalyst kept 80% of its initial activity after 15 cycles, being applied in the esterification of palmitic acid with ethyl alcohol.…”