Effect of additives on the viability of bifidobacteria loaded in alginate poly-l-lysine microparticles during the freeze-drying process

Cui, Jing; Qing, Cao; Choi, Yun Jaie; Lee, Kyung Hoon; Lee, Beom Jin

doi:10.1007/bf02968256

Cited by 12 publications

(13 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Removal of water during 2 steps of freeze drying (that is, primary drying and secondary drying) reduced the stability of protein and caused liposome fusion of bacterial membrane (Crowe and others 1993). Freeze drying for 24 h decreased the viability of bifidobacteria from initially >10 log CFU/g to 6.7 log CFU/g (Cui and others 2006). …”

Section: Resultsmentioning

confidence: 99%

Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature

Dianawati

Shah

2011

Journal of Food Science

View full text Add to dashboard Cite

Survival, acid and bile tolerance, and surface hydrophobicity of microencapsulated Bifidobacterium animalis ssp. lactis Bb12 were studied during storage at room temperature (25 °C) at low water activity (0.07, 0.1, and 0.2). Two types of alginate-based systems were prepared with and without mannitol as microencapsulant of B. animalis ssp. lactis Bb12. Formation of gel beads containing cells was achieved by dropping each emulsion into CaCl(2) solution; then, the beads were freeze dried. Survival, acid tolerance during 2-h exposure in de Man, Rogosa, Sharpe (MRS) broth at pH 2.0, bile tolerance during 8-h exposure in MRS broth containing taurocholic acid at pH 5.8, and retention of surface hydrophobicity were determined after freeze drying and during storage. The result showed that neither alginate nor alginate-mannitol formulation was effective in protecting B. animalis ssp. lactis Bb12 during freezing and freeze drying. The viability in alginate-mannitol and alginate formulations after freeze drying was 6.61 and 6.34 log CFU/g, respectively. Storage at low a(w) improved survival, acid tolerance, bile tolerance, and surface hydrophobicity retention of microencapsulated B. animalis ssp. lactis Bb12 when compared with controlled storage in an aluminum foil (with a(w) of 0.38 and 0.40 for alginate-mannitol and alginate formulations, respectively). Alginate mannitol was more effective than the alginate system during a short period of storage, but its effectiveness decreased during a long period of storage (80% survival at 10 wk). Nevertheless, storage of microencapsulated B. animalis ssp. lactis Bb12 in an aluminum foil without a(w) adjustment during 10 wk at room temperature was not effective (survival was 64% to 65%).

show abstract

Section: Resultsmentioning

confidence: 99%

Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature

Dianawati

Shah

2011

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…The literature reports microparticles both smaller and larger than those in this study. Smaller microparticles of average sizes between 150 nm and 84.8 lm [9,20,23] and larger microparticles of average sizes between 345.43 lm and 2 mm [8,18] were reported.…”

Section: Discussionmentioning

confidence: 97%

Characterisation of the Poly-(Vinylpyrrolidone)-Poly-(Vinylacetate-Co-Crotonic Acid) (PVP:PVAc-CA) Interpolymer Complex Matrix Microparticles Encapsulating a Bifidobacterium lactis Bb12 Probiotic Strain

Mamvura

Moolman

Kalombo³

et al. 2011

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

The method of producing poly-(vinylpyrrolidone)-poly-(vinylacetate-co-crotonic acid) (PVP:PVAc-CA) interpolymer complex matrix microparticles in supercritical carbon dioxide (scCO2), encapsulating bacteria, has recently been developed. This study was aimed at probing the external and internal structure of these microparticles, which can be used in food. The encapsulation efficiency and distribution of encapsulated Bifidobacterium lactis Bb12 within these microparticles were also investigated. Scanning electron microscopy (SEM) revealed irregular, mostly small, smooth microparticles with no visible bacterial cells on the surface. However, some of the microparticles appeared to have porous surfaces. The results of a Microtrac S3500 particle size analyzer showed that the PVP:PVAc-CA interpolymer complex matrix microparticles encapsulating B. lactis Bb12 had an average particle size of 166.1 μm (<350 μm designated standard size for microparticles). The D 10, D 50 and D 90 values for these microparticles were 48.16, 166.06 and 382.55 μm, respectively. Both SEM and confocal laser scanning microscopy showed a high density of bacterial cells within the microparticles. An average encapsulation efficiency of 96% was achieved. Consequently, the microparticles have the potential to be evenly distributed in foods, deliver adequate amounts of probiotics and produce minimal adverse effects on the texture and mouth feel of the foods into which they are incorporated.

show abstract

“…Moving away from laboratory scales, for larger alginate microgel production rates, a commercially available or custom‐made nozzle such as a coaxial air nozzle 2, 4, 10, 20, 28, 29, 35, 38, 39, 47–50, an effervescent (Turbotak) nozzle 17, 37, 51–54, a fat jet nozzle 5, 7, or a prefilming nozzle 26, 27 (Fig. 3) is preferred.…”

Section: Air Extrusion Methodsmentioning

confidence: 99%

Air Extrusion System for Ionotropic Alginate Microgel Particle Formation: A Review

2016

View full text Add to dashboard Cite

Ionotropic alginate microgels are commonly used for bioencapsulation applications and the air extrusion system is one of the common methods employed to produce these microgels. Although there have been many individual studies, to date no single report has comprehensively summarized the findings and results of the usage of this system. This review gives insight into the air extrusion methods, covering the setup, type, and characteristics of the system, focusing on their innovative aspects. Characterization techniques for the resulting microgels are presented, along with the influence of process variables on the product size and the development of empirical models for size prediction.

show abstract

Effect of additives on the viability of bifidobacteria loaded in alginate poly-l-lysine microparticles during the freeze-drying process

Cited by 12 publications

References 13 publications

Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature

Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature

Characterisation of the Poly-(Vinylpyrrolidone)-Poly-(Vinylacetate-Co-Crotonic Acid) (PVP:PVAc-CA) Interpolymer Complex Matrix Microparticles Encapsulating a Bifidobacterium lactis Bb12 Probiotic Strain

Air Extrusion System for Ionotropic Alginate Microgel Particle Formation: A Review

Contact Info

Product

Resources

About