Effect of additives on process stability of mesophilic anaerobic monodigestion of pig slaughterhouse waste

Bayr, Suvi; Pakarinen, Outi; Korppoo, Aki; Liuksia, Saku; Väisänen, Ari; Kaparaju, Prasad; Rintala, Jukka

doi:10.1016/j.biortech.2012.06.009

Cited by 55 publications

(17 citation statements)

References 25 publications

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“…The positive effect of micro nutrient supplementation in anaerobic digestion process is well known and has been described by many authors [53,54]. , who tested the impact of trace elements addition on mono-digestion of slaughterhouse waste, point out that such additives can facilitate achievement of higher OLRs [18]. This finding was confirmed in our lab investigations.…”

Section: Lab Scale Experiencesupporting

confidence: 78%

“…Even in lab-scale experiments on mono-digestion of slaughterhouse waste, the applied ammonia concentration ranges mostly between 3 and 5 g/kg [18,19,21,23,28] and severe inhibition is reported at levels of 7 g/kg [11,19,21].…”

Section: Lab Scale Experiencementioning

confidence: 98%

“…Some studies report on bio methane potential (BMP) of different waste fractions [10][11][12][13][14][15][16][17]. Besides that, continuous AD experiments at different process conditions (temperature, loading rate, influence of thermal pretreatment, NH 4 -removal) were conducted [11,[16][17][18][19][20][21][22].…”

Section: Generalmentioning

confidence: 99%

See 2 more Smart Citations

Energy self-supply of large abattoir by sustainable waste utilization based on anaerobic mono-digestion

et al. 2015

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Section: Lab Scale Experiencesupporting

confidence: 78%

Section: Lab Scale Experiencementioning

confidence: 98%

See 1 more Smart Citation

Energy self-supply of large abattoir by sustainable waste utilization based on anaerobic mono-digestion

et al. 2015

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“…These methanogens are, however, most vulnerable to different environmental and operational factors, including abrupt pH changes, organic overloading, and high salt and total ammonia nitrogen (TAN) concentrations, leading to the accumulation of volatile fatty acids (VFA) and subsequent process failure (Chen et al 2008;De Vrieze et al 2012). High TAN concentrations in AD often result from feeding the reactor with substrates with high protein content, such as slaughterhouse waste and manure (Bayr et al 2012;Hansen et al 1998). The toxic effect of TAN in AD can be attributed to the free ammonia (NH 3 ) fraction, which increases with increasing pH and temperature (Chen et al 2008;Hashimoto 1986;Schnurer and Nordberg 2008).…”

Section: Introductionmentioning

confidence: 99%

Inoculum selection is crucial to ensure operational stability in anaerobic digestion

Vrieze

Gildemyn

Vílchez-Vargas

et al. 2014

Appl Microbiol Biotechnol

116

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Anaerobic digestion is considered a key technology for the future bio-based economy. The microbial consortium carrying out the anaerobic digestion process is quite complex, and its exact role in terms of "elasticity", i.e., the ability to rapidly adapt to changing conditions, is still unknown. In this study, the role of the initial microbial community in terms of operational stability and stress tolerance was evaluated during a 175-day experiment. Five different inocula from stable industrial anaerobic digesters were fed a mixture of waste activated sludge and glycerol. Increasing ammonium pulses were applied to evaluate stability and stress tolerance. A different response in terms of start-up and ammonium tolerance was observed among the different inocula. Methanosaetaceae were the dominant acetoclastic methanogens, yet, Methanosarcinaceae increased in abundance at elevated ammonium concentrations. A shift from a Firmicutes to a Proteobacteria dominated bacterial community was observed in failing digesters. Methane production was strongly positively correlated with Methanosaetaceae, but also with Bacteria related to Anaerolinaceae, Clostridiales, and Alphaproteobacteria. Volatile fatty acids were strongly positively correlated with Betaproteobacteria and Bacteroidetes, yet ammonium concentration only with Bacteroidetes. Overall, these results indicate the importance of inoculum selection to ensure stable operation and stress tolerance in anaerobic digestion.

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“…When the OLR was increased further (to 6.5 kg· VS· m −3 ·d −1 ), there was still no VFA accumulation or signs of lower specific biogas production ( Figure 1A). Previous studies have shown that trace element addition has positive effects, especially on process stability, i.e., reduced VFA accumulation [9,10,18]. However, the effects of nickel and cobalt could not be separated from these experimental data and therefore nickel administration was terminated on day 89.…”

Section: Reactor Performancementioning

confidence: 99%

Determination of Methane and Carbon Dioxide Formation Rate Constants for Semi-Continuously Fed Anaerobic Digesters

2015

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Abstract:To optimize commercial-scale biogas production, it is important to evaluate the performance of each microbial step in the anaerobic process. Hydrolysis and methanogenesis are usually the rate-limiting steps during digestion of organic waste and by-products. By measuring biogas production and methane concentrations on-line in a semi-continuously fed reactor, gas kinetics can be evaluated. In this study, the rate constants of the fermentative hydrolysis step (kc) and the methanogenesis step (km) were determined and evaluated in a continuously stirred tank laboratory-scale reactor treating food and slaughterhouse waste and glycerin. A process additive containing Fe 2+ , Co 2+ and Ni 2+ was supplied until day 89, after which Ni 2+ was omitted. The omission resulted in a rapid decline in the methanogenesis rate constant (km) to 70% of the level observed when Ni 2+ was present, while kc remained unaffected. This suggests that Ni 2+ mainly affects the methanogenic rather than the hydrolytic microorganisms in the system. However, no effect was initially observed when using conventional process monitoring parameters such as biogas yield and volatile fatty acid concentration. Hence, formation rate constants can OPEN ACCESSEnergies 2015, 8 646 reveal additional information on process performance and km can be used as a complement to conventional process monitoring tools for semi-continuously fed anaerobic digesters.

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Effect of additives on process stability of mesophilic anaerobic monodigestion of pig slaughterhouse waste

Cited by 55 publications

References 25 publications

Energy self-supply of large abattoir by sustainable waste utilization based on anaerobic mono-digestion

Energy self-supply of large abattoir by sustainable waste utilization based on anaerobic mono-digestion

Inoculum selection is crucial to ensure operational stability in anaerobic digestion

Determination of Methane and Carbon Dioxide Formation Rate Constants for Semi-Continuously Fed Anaerobic Digesters

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