Effect of Additional Amino Group to Improve the Performance of Immobilized Lipase From Aspergillus niger by Adsorption-Crosslinking Method

Abstract

Adsorption-crosslinking is one of the immobilization methods to improve the reusability of lipase. It requires amino groups to reduce cross-link immobilization risk so that lipase–support interaction increases and the immobilization is attainable. Also, the amino group on the support is expected to increase lipase performance. This study aimed to analyze the effect of amino group addition on immobilized Aspergillus niger lipase by the adsorption-crosslinking using MP-64 macroporous anion resin and XAD-7HP macroporous nonionic resin that has been treated with chitosan. The chitosan-coated resin was characterized by Fourier transform infrared spectrometry (FTIR) and scanning electron microscope (SEM). Lipase immobilization was carried out by adding 10 ml lipase solution containing 0.75 g resins and shaken at 25°C for 150 rpm. Adsorption was achieved for 4 h, followed by cross-linking separately (adding 0.5% (v/v) glutaraldehyde and re-reacting for 20 min). Lipase activity was measured with the titrimetric of olive oil emulsion; mixed with Aspergillus niger lipase, emulsion, and a buffer solution (pH 6.5, ionic strength of 0.7); and incubated for 30 min at 37°C. The effect of amino-functional groups was investigated based on lipase loading and lipase activity. The best lipase loading and lipase activity of 83.79% and 29.41 U/g support were achieved in the adsorption-crosslinking using MP-64 resin coated with chitosan. After four cycles, biodiesel synthesis was maintained at 70.61% of the initial yield. These results indicated that chitosan as an affordable and readily available source of amino groups could be used to modify support for Aspergillus niger lipase immobilization.

A self-crosslinking nanogel scaffold for enhanced catalytic efficiency and stability

“…7). [68][69][70][71][72][73] This is possibly due to the conformational change of lipase that enhances the catalytic activity, consistent with the reported study. 63 Moreover, the hybrid nanogels with lipase at different concentrations show that the hydrolysis efficiency of pNPP increases obviously as the polymer concentration increases (Fig.…”

Review on sterilization techniques, and the application potential of phage lyase and lyase immobilization in fighting drug-resistant bacteria

“…Nowadays, there are various immobilization techniques, including traditional immobilization methods, such as adsorption, covalent bonding, entrapment, and cross-linking, 102,103 and new immobilization methods, such as adsorption-crosslinking, adsorption-embedding, crosslinking-embedding, etc. [104][105][106][107] These traditional immobilization techniques differ in interaction types and characteristics and supporting material forms and types (Table 1). Support binding is one of the most commonly used methods for immobilizing enzymes.…”

Research Progress on Lignocellulosic Biomass Degradation Catalyzed by Enzymatic Nanomaterials

“…K m and V max were 28.35 mmol/L and 147.06 μ/(g RC-CBD-GAD •min), respectively, and the half-life was 23 cycles. Hermansyah et al [109] immobilized Aspergillus Niger lipase on chitosan treated MP-64 macroporous anionic resin and XAD7HP macroporous Nonionic resin by an adsorption-crosslinking method. When the adsorption-crosslinking agent was adsorbed on chitosan-coated MP-64, the lipase loading (83.79%) and enzyme activity (29.41 U/g carrier) were the highest.…”

“…K m and V max were 28.35 mmol/L and 147.06 μ/(g RC‐CBD‐GAD ⋅min), respectively, and the half‐life was 23 cycles. Hermansyah et al [109] . immobilized Aspergillus Niger lipase on chitosan treated MP‐64 macroporous anionic resin and XAD7HP macroporous Nonionic resin by an adsorption‐crosslinking method.…”