Effect of Addition of Tiger Grouper (Epinephelus fuscoguttatus) Skin Collagen to Cork Fish (Channa striata) Albumin Ointment on Closure of Cuts

Abstract

Ointments are semisolid preparations intended for topical application to the skin or mucous membranes. The ointment formulation requires the presence of an ointment base, the ointment base itself is an inactive carrier substance from topical preparations that can be in the form of liquid or solid which brings the active ingredient to contact with the skin. The ointment base used in an ointment formulation must be inert in other words not to damage or reduce the therapeutic effect of the drug it contains. The results of the first study showed that differences in collagen concentrations affected the quality of albumin ointment, namely pH, viscosity, protein content, fat content, and water content. The best albumin ointment was obtained at 0.8% collagen concentration with the following results pH 6.8, viscosity 27980 cP, protein content 1.34%, fat content 88.29%, and water content 2.24%. The results of the amino acid profile test showed that the highest result was Glycine 5618.18 mg/kg and the lowest was L-Tyrosine <66.93 mg/kg. Then continued in the second study, namely amino acid profile testing, zinc levels and experimental mice, the results of observations on day 3, day 5, and day 7 showed that the best concentration of albumin ointment treatment underwent a wound closure process. 75% on the 7th day.