Effect of addition of hyaluronan to embryo culture medium on survival of bovine embryos in vitro following vitrification and establishment of pregnancy after transfer to recipients

Block, J.; Bonilla, L.; Hansen, Peter J.

doi:10.1016/j.theriogenology.2008.11.007

Cited by 53 publications

(35 citation statements)

References 39 publications

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“…Stojkovic et al [35] reported that a high concentration (6 mg/mL) increased the viscosity of the culture medium and improved development rates and cell numbers in bovine embryos produced in vitro. Block et al [19] found that the addition of 1 mg/mL of HA to the culture medium increased blastocyst rates at 7 dpf compared to a culture medium without HA, but no effect was reported at lower concentrations (0.1 and 0.5 mg/mL). In our study, HA was added at a concentration of 0.5 mg/mL.…”

Section: Discussionmentioning

confidence: 99%

“…It plays an important role in embryonic development; HA receptors (CD44) are expressed on the surface of bovine embryos at different stages of development [18]. The addition of HA to embryo culture media has been shown to increase the number of bovine embryos that develop to the blastocyst stage [18,19] and improve survival rates of in vitro bovine embryos after vitrification [19].…”

Section: Introductionmentioning

confidence: 99%

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In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy

et al. 2015

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a b s t r a c tThe aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-b1, and PDGF-BB), and other molecules with surfactant and embryotrophic properties, such as recombinant albumin (RA) and hyaluronan (HA). The first part of the study was dedicated to define the best combination of GFs-CYKs þ RA þ HA for optimal embryonic development. Next, we compared development rates and embryo quality (inner cell mass [ICM]-to-total cell number [TCN] ratio), and postthaw survival and hatching rates using this synthetic medium (T1) and a control medium: synthetic oviduct fluid þ BSA þ ITS (insulin, transferrin, and selenium). The blastocyst rates were significantly higher with T1 than those with the control at 7 and 8 days after fertilization. There was no significant difference in TCN or the ICM/TCN ratio between the two treatments. Survival and hatching rates 48 hours after thawing were similar for both treatments. Finally, nine embryo transfers were conducted using fresh and previously frozen Day-7 blastocysts to evaluate the in vivo viability of embryos produced in this synthetic medium; four gestations were obtained from six fresh embryos and one gestation from three frozen embryos. In conclusion, the fetal calf serum and BSA-free medium, supplemented with GFs-CYKs þ RA þ HA, improved embryo development and gave comparable ICM/TCN ratios and postthaw survival rates to the control with BSA. Fresh and frozen embryos produced in this medium are viable for embryo transfer. This fully synthetic method of embryo culture is a useful means of reducing the risk of disease transmission via embryo transfer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy

et al. 2015

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“…Short-term effects such as higher or lower cell number (Fischer-Brown et al 2002), altered sex ratio (Kimura et al 2005(Kimura et al , 2008, changes in developmental kinetics (Holm et al 2002), and lower tolerance to cryopreservation and long-term effects such as large offspring syndrome (Young et al 1998) and increased frequencies of abnormalities and spontaneous abortion (Taverne et al 2002) are well documented. The mechanistic causes of (Menck et al 1997, de Moraes & Hansen 1997, Guyader-Joly et al 1999, Luciano et al 1999, Stojkovic et al 2002, Alm et al 2005, Fujita et al 2006, Block et al 2009, Pereira et al 2010. However, blastocyst yield might not be an appropriate measurement of IVP efficiency if embryo quality is defined as the potential for healthy gestation and successful coping with stresses such as cryopreservation and transfer protocols.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive cross production system assessment of the impact of in vitro microenvironment on the expression of messengers and long non-coding RNAs in the bovine blastocyst

Côté

Vigneault²,

Laflamme³

et al. 2011

Reproduction

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In vitro production (IVP) of cattle embryos over the past two decades has revealed several negative impacts that have been attributed to the artificial microenvironment. Studies on embryos produced in vitro clearly point to aberrant gene expression levels. So far, the causal association between phenotype and measured gene expression has not led to substantial improvement of IVP systems. The aim of this study was to generate a unique dataset composed of microarray-derived relative transcript abundance values for blastocysts produced in ten in vitro systems differing primarily in culture medium formulation. Between-group comparisons determine the level of overall similarity among systems relative to in vivo reference embryos. The use of the dataset to contrast all in vitro treatments with the in vivo blastocysts pointed to a single common gene network. The 'boutique' array contained a panel of novel uncharacterized transcripts that were variably expressed depending on the medium in which the blastocysts were produced. These novel transcripts were differentially expressed in blastocysts even as carryover from conditions encountered 7 days earlier during oocyte maturation. All of the selected novel candidates thus expressed were from intergenic regions. The function of this long non-coding RNA remains unknown but clearly points to an additional level of complexity in early embryo development.

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“…On the other hand, in vitro development of zona-free 8-cell embryos in the presence of HA or HS significantly decreased the incidence of apoptotic cells and consequently increased the mean cell number in the blastocyst, but could not improve the incidences of embryos implanted and developed to term after embryo transfer. In mice and cattle, culture of intact embryos in the presence of HA (0.5-1.0 mg/ml) increased implantation rates and fetal development following transfer [18,52]. In the present study, although the presence of HA or HS improved the cell number of embryos, due to the absence of the zona pellucida, the number may be still insufficient for successful implantation after transfer of the embryos.…”

mentioning

confidence: 47%

Glycosaminoglycans Improves Early Development of Zona-free 8-cell Rat Embryos to Blastocysts in a Chemically Defined Medium, but Not the Pregnancy Rate Following Transfer of the Blastocysts

Okuyama

Funahashi

2012

Journal of Reproduction and Development

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Abstract. The objective of the present study was to clarify the possible role of the zona pellucida (ZP) in early development of rat embryos and to determine the effect of glycosaminoglycans on the development of ZP-free 8-cell embryos before or after embryo transfer at the blastocyst stage. Eight-cell embryos were divided into three groups comprised of, 1) intact controls, 2) embryos with the ZP was removed with acidic solution and 3) pairs of ZP-free 8-cell embryos aggregated in a small hollow. These embryos were cultured in a chemically defined mR1ECM for 24 h. Developmental ability to the blastocyst stage and mean cell number in the blastocyst was lower in ZP-free embryos than in intact controls. When these blastocysts were transferred, the farrowing rate and efficiency of embryos developed to term were also lower in ZP-free embryos, but not in the aggregated ones. Supplementation with hyaluronan (HA; 63-250 μg/ml) or heparan sulfate proteoglycan (HS; 15 μg/ml) significantly improved blastocyst formation of ZP-free embryos and the cell number in the blastocyst by reducing the incidence of apoptosis. However, there were no beneficial effects of HA or HS on farrowing and newborn rates after transfer of the blastocysts. In conclusion, the ZP plays roles in maintaining successful development of early rat embryos at least from the 8-cell stage not only to the blastocyst stage but also to posttransfer stages. Glycosaminoglycans, such as HA or HS, appear to contribute to successful cleavage during early development to the blastocyst stage but may be insufficient to maintain the posttransfer survival of ZP-free embryos. Key words: Blastocyst, Embryo culture, Glycosaminoglycans, Rat, Zona pellucida (J. Reprod. Dev. 58: [295][296][297][298][299][300][301] 2012) M ammalian oocytes, zygotes and early-to blastocyst-stage embryos are enclosed by a thick layer of glycoproteins, called the zona pellucida, which is produced in the early stage of oogenesis. The zona is known to regulate sperm binding to the ovulated oocytes and the monospermic penetration, to prevent separation of blastomeres or aggregation of different embryos during early development, and to protect against viral, bacterial and fungous pathogens or immune cells in the female reproductive tract [1,2].Recent developments in embryo culture technology have made it possible, in many species, for cleavage embryos to develop efficiently to the blastocyst stage in a chemically defined medium with successful results to term following transfer of the blastocysts [3][4][5]. In rats, a chemically defined culture medium, rat 1-cell embryo culture medium (R1ECM), has been developed and succeeded in achieving early development of in vivo and in vitro fertilized rat zona-intact zygotes to the blastocyst stage [6,7]. Gametes and embryos of the rat, one of the standard experimental rodents, have been utilized not only for biomedical research but also for biotechnological attempts including in vitro fertilization [7][8][9], embryo culture [6,10] and manipulation of gametes...

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Effect of addition of hyaluronan to embryo culture medium on survival of bovine embryos in vitro following vitrification and establishment of pregnancy after transfer to recipients

Cited by 53 publications

References 39 publications

In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy

In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy

Comprehensive cross production system assessment of the impact of in vitro microenvironment on the expression of messengers and long non-coding RNAs in the bovine blastocyst

Glycosaminoglycans Improves Early Development of Zona-free 8-cell Rat Embryos to Blastocysts in a Chemically Defined Medium, but Not the Pregnancy Rate Following Transfer of the Blastocysts

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