Effect of adding crude glycerine to diets with feed additives on the feed intake, ruminal degradability, volatile fatty acid concentrations and in vitro gas production of feedlot Nellore cattle

Almeida, Márco Túlio Costa; Paschoaloto, Josimari Regina; Perez, Henrique Leal; Carvalho, Vanessa Barbosa de; Homem, Antônio Carlos; Fávaro, Vanessa Ruiz; Blair, Hugh T.

doi:10.1111/jpn.13105

Cited by 6 publications

(2 citation statements)

References 57 publications

(82 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glycerol is widely used as a feed supplement in small ruminant nutrition, both as replacer of corn grain in the diet [1][2][3][4][5] and to increase productive performance [6][7][8][9][10][11][12]. Glycerol is a hyperosmotic agent, lipid soluble, and it can pass cell membrane by simple diffusion, following a concentration gradient [13,14].…”

Section: Introductionmentioning

confidence: 99%

Effect of Media with Different Glycerol Concentrations on Sheep Red Blood Cells’ Viability In Vitro

Pasciu

Sotgiu

Porcu

et al. 2021

Animals

View full text Add to dashboard Cite

The use of high doses of glycerol as a livestock feed supplement is followed by a rapid increase in plasma concentrations and consequently in plasma osmolality. Moreover, glycerol is a highly diffusible molecule that can readily permeate the red blood cell (RBC) membrane following a concentration gradient. A rise in glycerol plasma concentrations can thus alter RBC homeostasis. The present study aimed at investigating both glycerol osmotic effects on sheep RBCs and their oxidative response under in vitro conditions. Sheep blood samples were suspended in media supplemented with increasing glycerol concentrations (0, 25, 50, 100, 150, 200, 250, 300, 350, 400 mg/dL), which reflected those found in vivo in previous studies, and incubated at 37 °C for 4h. Thereafter, osmolality and hemolysis were determined in spent media, while cell extracts were used to assay intracellular concentration of glycerol, ATP, Ca2+ ions, oxidative stress markers and reactive oxygen species (ROS).The study confirmed that glycerol intracellular concentrations are directly related with its concentration in the incubation media, as well as hemolysis (p < 0.001) which increased significantly at glycerol concentrations higher form 200 mg/dL. ROS intracellular level increased at all glycerol concentration tested (p < 0.01) and total thiols decreased at the highest concentrations. However, RBCs proved to be able to cope by activating their antioxidant defense system. Superoxide dismutase activity indeed increased at the highest glycerol concentrations (p < 0.001), while total antioxidant capacity and malonyldialdehyde, a typical product of lipid peroxidation by ROS, did not show significant changes. Moreover, no alterations in intracellular Ca2+ ions and ATP concentrations were found. In conclusion, glycerol-induced hemolysis can be related to the induced osmotic stress. In sheep, nutritional treatments should be designed to avoid reaching glycerol circulating concentrations higher than 200 mg/dL.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of Media with Different Glycerol Concentrations on Sheep Red Blood Cells’ Viability In Vitro

Pasciu

Sotgiu

Porcu

et al. 2021

Animals

View full text Add to dashboard Cite

show abstract

“…In small ruminants, glycerol, alone or in association with propylene glycol, has been used both as replacer of corn grain in the diet [5][6][7][8][9] and as a feed supplement to increase productive performance [10][11][12][13][14][15][16][17]. In these studies, glycerol was supplemented at a percentage of inclusion on dry matter (DM) basis ranging from 3% up to 45%.…”

Section: Introductionmentioning

confidence: 99%

Administration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affected

et al. 2020

View full text Add to dashboard Cite

Background The objective of this study was to investigate the metabolic and osmotic effects of different doses of glycerol or a glycerol – propylene glycol mixture in Sarda sheep with the aim to identify those able to beneficially modify ewe’s metabolic status without harmful changes in red blood cell (RBC) indices. Thereafter, the selected doses were tested for their effects on ewe’s ovarian activity during an induced follicular phase and compared to the effects of a hormonal treatment with equine chorionic gonadotrophin (eCG). Results Glycerol was administered alone (G groups: 90% glycerol and 10% water; % v/v) or in combination with propylene glycol (M groups: 70% glycerol, 20% propylene glycol, 10% water; % v/v). Treatments were formulated to provide 100, 75, 50 and 25% of the amount of energy supplied in previous experiments. Obtained results showed that the formulations G75 and M75 (22.5 and 18.2% on DM basis, respectively) induce metabolic changes comparable to those induced by M100. The latter dose has been already evaluated for its effects on sheep metabolism and reproductive performance. However, with these high doses, plasma osmolality increased significantly, and RBC indices showed significant alterations. The low dose groups (G25 and M25, 8.6 and 6.9% on DM basis, respectively) did not show any alterations in plasma osmolality and RBC indices, but the metabolic milieu differed markedly from that of M100. Between the medium dose groups, M50 (12.9% on DM basis) showed a more comparable milieu to M100 than G50 (15.9% on DM basis) and no RBC alterations. Therefore, M75, G75 and M50 doses were tested for their effect on ovarian functions and proved to be equally effective as eCG. Conclusion The results of the present study evidenced an alteration of RBC indices, and possibly of their functions, as a side effect of glycerol administration at high doses in the diet of ewes. Therefore, protocols foreseeing the administration of glycerol should be tested for their effects on RBC indices and functions. In general terms, the medium dose of the glucogenic mixture (12.9% of dietary DM on offer) should be preferred.

show abstract

Meta-analysis of the effects of essential oils on consumption, performance, and ruminal fermentation of beef cattle

Ferreira,

Fernandes,

Lobo Júnior

et al. 2024

Animal Feed Science and Technology

View full text Add to dashboard Cite

Effect of adding crude glycerine to diets with feed additives on the feed intake, ruminal degradability, volatile fatty acid concentrations and in vitro gas production of feedlot Nellore cattle

Cited by 6 publications

References 57 publications

Effect of Media with Different Glycerol Concentrations on Sheep Red Blood Cells’ Viability In Vitro

Effect of Media with Different Glycerol Concentrations on Sheep Red Blood Cells’ Viability In Vitro

Administration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affected

Meta-analysis of the effects of essential oils on consumption, performance, and ruminal fermentation of beef cattle

Contact Info

Product

Resources

About