Effect of acyclic polyisoprenoids on the biosynthesis of mannose-labeled glycolipids in rat liver microsomes

Murakami, Manabu; Muto, Yasutoshi; Moriwaki, Hisataka; Ohgo, Toshiharu

doi:10.1016/0006-291x(81)90507-6

Cited by 21 publications

(7 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are compatible with the pre vious report that GGA prevented the gastric hexosamine content from its reduction by the cold restraint stress in rat [1] and that GGA is involved in glycoprotein synthesis through glycosyltransferasc in the gastric mucosa [9], Furthermore, we tested whether this ac tion of GGA was mediated by endogenous prostaglandins of gastric mucosa, since pros taglandins were reported to stimulate mucus production [5,8]. Hiraislii et al [10] demon strated that the cultured cells employed in this study were able to produce prostaglandin E2 as well as K In this study, therefore, we tried to investigate whether GGA could in crease prostaglandin synthesis by the cul tured cells.…”

Section: Discussionsupporting

confidence: 92%

Geranylgeranylacetone, a Novel Anti-Ulcer Drug, Stimulates Mucus Synthesis and Secretion in Rat Gastric Cultured Cells

Terano¹,

Hiraishi²,

Ota³

et al. 1986

Digestion

View full text Add to dashboard Cite

Aim of this study was to investigate the effect of geranylgeranylacetone (GGA) on mucus synthesis and secretion in rat gastric cultured cells, and their relationship to prostaglandin (PG) synthesis. Rate of mucus synthesis was estimated by incorporation of ³H-glucosamine into the cultured cells. Release of ³H-glucosamine from the cells, which were preincubated in the medium containing the radioactive isotope, into the culture media was measured for the evaluation of mucus secretion. PG production by the cultured cells was measured by radioimmunoassay. GGA increased glycoprotein synthesis in a dose-dependent manner (p < 0.01). Secretion of mucus from cultured cells was also significantly enhanced by GGA. GGA did not significantly increase PG (E₂ and I₂production. These results indicate that GGA has the ability to stimulate mucus production by the gastric epithelial cells, and this action may play an important role in protective effect of GGA. It is, however, unlikely that this effect of GGA is mediated by endogenous PGs.

show abstract

Section: Discussionsupporting

confidence: 92%

Geranylgeranylacetone, a Novel Anti-Ulcer Drug, Stimulates Mucus Synthesis and Secretion in Rat Gastric Cultured Cells

Terano¹,

Hiraishi²,

Ota³

et al. 1986

Digestion

View full text Add to dashboard Cite

show abstract

“…By the treatment with aspirin, hexosamine contents in both tissues of the corpus and antrum were markedly reduced, and also the hexosamine concentrations in both tissues decreased from a normal value of 15 In the antrum, GGA also prevented the reduction of hexosamine .01 when compared with the control. content, but not significantly.…”

Section: Resultsmentioning

confidence: 87%

Effects of the antiulcer drug geranylgeranylacetone on aspirin-induced gastric ulcers in rats.

et al. 1982

Self Cite

View full text Add to dashboard Cite

Abstract-Antiulcer effects of geranylgeranylacetone (GGA) on aspirin induced gastric ulcers in rats were studied, comparing them with those of gefarnate. The oral administration of GGA prevented the development of gastric ulcer induced by a single or repeated oral administration (5 consecutive days) of aspirin. The effects of GGA were more potent and more definite than those of gefarnate. The intraduodenal administration of GGA, but not the intragastrical administration, also inhibited the ulceration induced by aspirin in pylorus-ligated rats, while the intraduodenal adminis tration of gefarnate did not. GGA prevented the reduction of the H+ concentration and the increment of Na+ concentration in the gastric juice induced by aspirin. In addition, the decrease of hexosamine content in the gastric mucosa induced by aspirin was restored to a normal level by GGA, but not by gefarnate. From these results, it was concluded that the protective actions of GGA on aspirin-induced gastric ulcers might be due to its pro tection from the weakening of gastric mucosal resistances.

show abstract

“…The preventive effect of GGA on the decreases in gastric glycoproteins may be attributed to action on the glycoprotein biosynthesis in the gastric mucosa since it was suggested that GGA might be involved in the glycoprotein synthesis through the enhancement of glycolipid biosynthesis in rat liver microsomes (13).…”

Section: Discussionmentioning

confidence: 99%

Effect of geranylgeranylacetone on aspirin-induced changes in gastric glycoproteins.

Oketani¹,

Murakami²,

Fujisaki³

et al. 1983

Jpn.J.Pharmacol

Self Cite

View full text Add to dashboard Cite

Abstract-The effects of aspirin and treatment with geranylgeranylacetone (GGA), an antiulcer drug, on the content of gastric glycoproteins were investigated in pylorus ligated rats. In normal rats, the amount of gastric glycoproteins in the mucous layer was about 1.5 times higher than that in the gastric mucosa, indicating that the glycoproteins were distributed in the mucous layer as a highly concentrated state. Aspirin (100 mg/kg, p.o.) decreased the content of gastric glycoproteins both in the mucous layer and in the gastric mucosa. The amount of the macromolecular fraction in the gastric juice, which corresponded to the gastric glycoproteins on the basis of molecular size, was not affected by aspirin. GGA (300 mg/kg, i.d.) could prevent the decreases of the total amount of gastric glycoproteins in the mucous layer plus gastric mucosa. These results indicated that the glycoproteins coating the surface of the gastric mucosa may play a role as a defensive mechanism and that GGA exerted an antiulcer effect on aspirin induced mucosal damage through preventing the decreases in gastric glycoproteins.

show abstract

Effect of acyclic polyisoprenoids on the biosynthesis of mannose-labeled glycolipids in rat liver microsomes

Cited by 21 publications

References 5 publications

Geranylgeranylacetone, a Novel Anti-Ulcer Drug, Stimulates Mucus Synthesis and Secretion in Rat Gastric Cultured Cells

Geranylgeranylacetone, a Novel Anti-Ulcer Drug, Stimulates Mucus Synthesis and Secretion in Rat Gastric Cultured Cells

Effects of the antiulcer drug geranylgeranylacetone on aspirin-induced gastric ulcers in rats.

Effect of geranylgeranylacetone on aspirin-induced changes in gastric glycoproteins.

Contact Info

Product

Resources

About