Effect of acute heat shock on stress gene expression and DNA methylation in zebu (Bos indicus) and crossbred (Bos indicus × Bos taurus) dairy cattle

Masroor, Sana; Aalam, Mohd Tanzeel; Khan, Owais; Tanuj, Gunturu Narasimha; Gandham, Ravi Kumar; Dhara, Sujoy K.; Gupta, Praveen Kumar; Mishra, Bishnu Prasad; Dutt, Triveni; Singh, G. R.; Sajjanar, Basavaraj

doi:10.1007/s00484-022-02320-3

Cited by 5 publications

(3 citation statements)

References 96 publications

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“…Heat stress may activate the modulatory effects of RHEB on the mTOR pathway, thereby registering proliferation and autophagy in dairy mammary cells. As for NR3C1 (glucocorticoid receptor), it was found to reduce the promoter methylation under heat stress in dairy cattle [ 48 ]. Another interesting finding is that the expression of circRNA22544 (EZH2) significantly differed between Sum1 and Spr1 [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of CircRNA Expression in Peripheral Blood of Holstein Cows in Response to Heat Stress

Zhang

Wang

et al. 2023

IJMS

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The present study aimed to identify key circRNAs and pathways associated with heat stress in blood samples of Holstein cows, which will provide new insights into the molecular mechanisms driving heat stress in cows. Hence, we evaluated changes in milk yield, rectal temperature, and respiratory rate of experimental cows between heat stress (summer) and non-heat stress (spring) conditions with two comparisons, including Sum1 vs. Spr1 (same lactation stage, different individuals, 15 cows per group) and Sum1 vs. Spr2 (same individual, different lactation stages, 15 cows per group). Compared to both Spr1 and Spr2, cows in the Sum1 group had a significantly lower milk yield, while rectal temperature and respiratory rate were significantly higher (p < 0.05), indicating that cows in the Sum1 group were experiencing heat stress. In each group, five animals were chosen randomly to undergo RNA-seq. The results reveal that 140 and 205 differentially expressed (DE) circRNAs were screened in the first and second comparisons, respectively. According to the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, these DE circRNAs were mainly enriched in five signaling pathways, including choline metabolism, the PI3K/AKT signaling pathway, the HIF-1 signaling pathway, the longevity-regulating pathway, and autophagy. Then, we obtained the top 10 hub source genes of circRNAs according to the protein–protein interaction networks. Among them, ciRNA1282 (HIF1A), circRNA4205 (NR3C1), and circRNA12923 (ROCK1) were enriched in multiple pathways and identified as binding multiple miRNAs. These key circRNAs may play an important role in the heat stress responses of dairy cows. These results provide valuable information on the involvement of key circRNAs and their expression pattern in the heat stress response of cows.

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Section: Discussionmentioning

confidence: 99%

Analysis of CircRNA Expression in Peripheral Blood of Holstein Cows in Response to Heat Stress

Zhang

Wang

et al. 2023

IJMS

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“…Isolation of PBMC from blood samples of experimental animals and heat shock treatment were done as per the procedures explained in the earlier studies [ 30 ]. Briefly, the blood samples were collected from experimental animals in the morning hours (10.00 am) using sodium heparin coated vacutainers.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide expression analysis reveals different heat shock responses in indigenous (Bos indicus) and crossbred (Bos indicus X Bos taurus) cattle

et al. 2023

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Environmental heat stress in dairy cattle leads to poor health, reduced milk production and decreased reproductive efficiency. Multiple genes interact and coordinate the response to overcome the impact of heat stress. The present study identified heat shock regulated genes in the peripheral blood mononuclear cells (PBMC). Genome-wide expression patterns for cellular stress response were compared between two genetically distinct groups of cattle viz., Hariana (B. indicus) and Vrindavani (B. indicus X B. taurus). In addition to major heat shock response genes, oxidative stress and immune response genes were also found to be affected by heat stress. Heat shock proteins such as HSPH1, HSPB8, FKB4, DNAJ4 and SERPINH1 were up-regulated at higher fold change in Vrindavani compared to Hariana cattle. The oxidative stress response genes (HMOX1, BNIP3, RHOB and VEGFA) and immune response genes (FSOB, GADD45B and JUN) were up-regulated in Vrindavani whereas the same were down-regulated in Hariana cattle. The enrichment analysis of dysregulated genes revealed the biological functions and signaling pathways that were affected by heat stress. Overall, these results show distinct cellular responses to heat stress in two different genetic groups of cattle. This also highlight the long-term adaptation of B. indicus (Hariana) to tropical climate as compared to the crossbred (Vrindavani) with mixed genetic makeup (B. indicus X B. taurus).

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“…In a study comparing DNA methylation patterns in Indian cattle (Bos indicus) and crossbred cattle, there were differences in the expression patterns of DNA methyltransferases (DNMT1, DNMT3A) and demethylases (TET1, TET2 and TET3). Low methylation levels promoted the expression of the stress genes HSP70 and STP1, suggesting a role for DNA methylation in the regulation of the heat stress response pathway [133]. DNA methylation is also involved in the occurrence of dairy cow mastitis.…”

Section: Studies On Dna Methylation Associated With Resistance Perfor...mentioning

confidence: 98%

Effects of DNA Methylation on Gene Expression and Phenotypic Traits in Cattle: A Review

Zhang

Sheng

et al. 2023

IJMS

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Gene expression in cells is determined by the epigenetic state of chromatin. Therefore, the study of epigenetic changes is very important to understand the regulatory mechanism of genes at the molecular, cellular, tissue and organ levels. DNA methylation is one of the most studied epigenetic modifications, which plays an important role in maintaining genome stability and ensuring normal growth and development. Studies have shown that methylation levels in bovine primordial germ cells, the rearrangement of methylation during embryonic development and abnormal methylation during placental development are all closely related to their reproductive processes. In addition, the application of bovine male sterility and assisted reproductive technology is also related to DNA methylation. This review introduces the principle, development of detection methods and application conditions of DNA methylation, with emphasis on the relationship between DNA methylation dynamics and bovine spermatogenesis, embryonic development, disease resistance and muscle and fat development, in order to provide theoretical basis for the application of DNA methylation in cattle breeding in the future.

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Effect of acute heat shock on stress gene expression and DNA methylation in zebu (Bos indicus) and crossbred (Bos indicus × Bos taurus) dairy cattle

Cited by 5 publications

References 96 publications

Analysis of CircRNA Expression in Peripheral Blood of Holstein Cows in Response to Heat Stress

Analysis of CircRNA Expression in Peripheral Blood of Holstein Cows in Response to Heat Stress

Genome-wide expression analysis reveals different heat shock responses in indigenous (Bos indicus) and crossbred (Bos indicus X Bos taurus) cattle

Effects of DNA Methylation on Gene Expression and Phenotypic Traits in Cattle: A Review

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