Effect of active immunization using a novel GnRH vaccine on reproductive function in rats

Yao, Zhiqiu; Si, Wenyu; Tian, Weiguo; Ye, Jing; Zhu, Rongfei; Li, Xiumei; Ji, Shichun; Qin, Zheng; Liu, Ya; Fang, Fugui

doi:10.1016/j.theriogenology.2018.01.013

Cited by 12 publications

(15 citation statements)

References 38 publications

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“…Non-responders are frequently identified in several animal species, including rats, after treatment with several types of GnRH-I-based immunocontraceptive vaccines [21,[24][25][26]. Approximately 20-40% of rats and 33% of cats vaccinated with immunocontraceptive vaccines have been shown to be non-responders [21,24,25]. Similar results were found in this study.…”

Section: Discussionsupporting

confidence: 87%

“…Although the testis weights of these non-responders were slightly reduced, they were not statistically different from the testis weights of the negative control rats. Non-responders are frequently identified in several animal species, including rats, after treatment with several types of GnRH-I-based immunocontraceptive vaccines [21,[24][25][26]. Approximately 20-40% of rats and 33% of cats vaccinated with immunocontraceptive vaccines have been shown to be non-responders [21,24,25].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Immunocontraceptive Effects in Male Rats Vaccinated with Gonadotropin-Releasing Hormone-I and -II Protein Complex

Kim

Park

Ahn

et al. 2019

Journal of Microbiology and Biotechnology

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Immunocontraceptive Effects in Male Rats Vaccinated with Gonadotropin-Releasing Hormone-I and -II Protein Complex

Kim

Park

Ahn

et al. 2019

Journal of Microbiology and Biotechnology

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“…Immunocastration creates specific antibodies that recognize and bond to the hormone GnRH-I, preventing bonding with pituitary receptors, thus inhibiting FSH and LH secretion, causing infertility [10,12]. Thus, immunocastration has proven inhibition of reproductive activity in pigs [13][14][15], cattle [16,17], horses [18], sheep [19], mice [20][21][22][23], camelids [24], cats [25], chickens [26], and dogs [9,27,28]. Bansal and collaborators (2019) have recently developed and proven that immunogenicity of a vaccine able to develop immunity against GnRH and rabies virus simultaneously in mice [29].…”

Section: Introductionmentioning

confidence: 99%

Reproductive and Behavioral Evaluation of a New Immunocastration Dog Vaccine

Siel

Ubilla

Vidal

et al. 2020

Animals

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Canine immunocastration development has been of interest for many years as a complementary strategy to surgical castration. The purpose of this paper was to verify the effect of a recombinant vaccine for dog immunocastration. Two tests were done, one under controlled conditions and a second under field conditions. Animals were injected with 1 mL of 500 µg GnRXG/Q recombinant protein; 500 µg of low molecular weight chitosan as adjuvant; 1 mL NaCl 0.9% q.s. In the first trial, eight Beagle male dogs between the ages of 1 and 3 comprised the sample, randomly divided into two groups: vaccinated group (n = 7) and control group (n = 2). The second trial had 32 dogs with owners. In the first controlled conditions trial, the vaccine produced specific antibodies that remained until the end of the trial (day 270), inducing reduced testosterone and spermiogram changes in the immunized animals. In a second trial, on the field, specific immunity was induced, which remained high up to day 150. The vaccine also reduced sexual agonistic and marking behaviors. This new vaccine proved to be safe, immunogenic, capable of reducing gonadal functionality, and had a positive effect on inducing reduced sexual, agonistic, and marking behavior of the animals.

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“…Moreover, modified Freund's complete adjuvant was less efficient than CpG ODN 2006 in Freund's incomplete adjuvant, which was administered to female rats with two booster injections and higher antibody concentration (Conforti et al, 2007). Moreover, there was a recent study proved that GnRH2-MAP vaccine can affect the reproductive function of Sprague-Dawley (SD) male rats by influencing the endogenous GnRH (Yao et al, 2018).…”

Section: Research Articlementioning

confidence: 99%

“…All experimental rats were randomly divided into ISA 201 adjuvant group (n=36), Gel 01 adjuvant group (n=36) and control group (n=36). The ISA 201 adjuvant group was inoculated with a mixture of ISA 201 adjuvant and GnRH2-MAP; Gel 01 adjuvant group was inoculated with a mixture of Gel 01 auxiliary and GnRH2-MAP; control group was inoculated with a mixture of physiological saline and GnRH2-MAP ,per rat was injected with mixture at a dose of 1 ml of which GnRH-MAP was 100 μg (Han et al, 2015;Yao et al, 2018). The first immunization was administered via subcutaneous injection in many sites of the back of 20 days rats, followed by two booster doses at 2-week intervals, with a total immunization of 3 times per rat.…”

Section: Immunization Products and Proceduresmentioning

confidence: 99%

Effect of Active Immunization Using Gonadotropin-Releasing Hormone 2-Multiple Antigen Peptide with Different Adjuvants on the Reproductive Functions of Male Rats

Si¹

2019

PVJ

Self Cite

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This study was aimed to compare the effects of different adjuvants on male reproductive function in rats immunized using GnRH2-MAP. Male Sprague-Dawley rats were assigned to control, ISA 201 adjuvant and Gel 01 adjuvant groups along with GnRH2-MAP. The serum testosterone and antibody titers were measured every 2-week by ELISA, the testes were weighed and histologically analyzed. mRNA expression levels in the hypothalamus, pituitary, and testes were determined by RT-qPCR. Among the three groups, the ISA 201 adjuvant group exhibited the lowest testosterone concentration (P<0.05), the highest antibody titer (P<0.05), and testis atrophy. GnRH mRNA expression in the hypothalamus; GnRH-R, FSH-β, and LH-β expression in the pituitary; and FSH-R, LH-R, and 3β-HSD expression in the testes were significantly lower in this group (P<0.01). Additionally, several other genes showed significant differential expression in the ISA 201 adjuvant group than in the other two groups. It is concluded that the GnRH2-MAP vaccine emulsified with ISA 201 adjuvant is more effective than that administered with Gel 01 adjuvant. , 2019. Effect of active immunization using gonadotropin-releasing hormone 2-multiple antigen peptide with different adjuvants on the reproductive functions of male rats. Pak Vet J, 39(2): 205-210. http://dx.

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Effect of active immunization using a novel GnRH vaccine on reproductive function in rats

Cited by 12 publications

References 38 publications

Immunocontraceptive Effects in Male Rats Vaccinated with Gonadotropin-Releasing Hormone-I and -II Protein Complex

Immunocontraceptive Effects in Male Rats Vaccinated with Gonadotropin-Releasing Hormone-I and -II Protein Complex

Reproductive and Behavioral Evaluation of a New Immunocastration Dog Vaccine

Effect of Active Immunization Using Gonadotropin-Releasing Hormone 2-Multiple Antigen Peptide with Different Adjuvants on the Reproductive Functions of Male Rats

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