Effect of Actinomycin D on Cellular Nucleic Acid Synthesis and Virus Production

Reich, E.; Franklin, Richard M.; Shatkin, Aaron J.; Tatum, E. L.

doi:10.1126/science.134.3478.556

Cited by 573 publications

(103 citation statements)

References 7 publications

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“…The results presented here demonstrate that APF induces not only a strong cell aggregation but also a distinct cell adhesiveness, characterized by development of well-defined binding structures (Reich et al, 1961;Anservin, 1965).…”

Section: Discussionmentioning

confidence: 58%

The induction of tumour cell adhesiveness and intercellular junctions by a glycoprotein of rat ascites hepatoma cell surface

Ishimaru¹,

Kudo²,

Ishihara³

et al. 1976

Br J Cancer

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Section: Discussionmentioning

confidence: 58%

The induction of tumour cell adhesiveness and intercellular junctions by a glycoprotein of rat ascites hepatoma cell surface

Ishimaru¹,

Kudo²,

Ishihara³

et al. 1976

Br J Cancer

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“…Transcription activity of HB4C5 cells was suppressed by Act D, which irreversibly inhibits transcription activity (Reich et al 1961). HB4C5 cells were treated with 4.0 g/ml of Act D for 2 h. Following Act D treatment, cells were washed twice by ERDF medium and inoculated at 1 Â 10 5 cells/ml to examine the IPSF effect of LDH on transcription-suppressed HB4C5 cells.…”

Section: Inhibition Of Protein Synthesismentioning

confidence: 99%

Lactate dehydrogenase enhances immunoglobulin production by human hybridoma and human peripheral blood lymphocytes

Takenouchi

Sugahara

2003

Cytotechnology

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Lactate dehydrogenase (LDH) derived from rabbit muscle enhanced IgM production by human-human hybridoma HB4C5 cells 12.4-fold at 320 g/ml under serum-free conditions. LDHs from pig muscle and pig heart also accelerated IgM production 8.4-and 6.4-fold, respectively. The immunoglobulin production stimulating activity of LDH was not accompanied by activation of cell proliferation. LDH from rabbit muscle facilitated IgM and IgG production by human peripheral blood lymphocytes. This means LDH stimulates immunoglobulin production not only by the specified hybridoma cell line, but also by unspecified immunoglobulin producers. LDH from rabbit muscle enhanced IgM production of transcription-suppressed HB4C5 cells treated with actinomycin D. The immunoglobulin production-stimulating factors (IPSFs) effect of LDH was slightly weakened by sodium fluoride (translation inhibitor) treatment of HB4C5. Moreover, the amount of intracellular IgM of monensin-treated HB4C5 cells was obviously enhanced by LDH. This result means that the IPSF effect of LDH is irrelevant to the post-translation activity of target cells. It is expected from these findings that LDH from rabbit muscle accelerates the translation step to enhance immunoglobulin productivity. The immunoglobulin production-stimulating activity of LDH was inhibited by colchicine, endocytosis inhibitor. This fact suggests that it is necessary for LDH to be taken by target cells to act as an IPSF.

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“…DNA synthesis is not affected materially by low concentrations of AMD (4). Actinomycin apparently binds to DNA (5), probably specifically with guanine (6,7), in bringing about its biological effects.…”

mentioning

confidence: 95%

The Effects of Actinomycin D on the Ultrastructure of the Nucleus of the Amphibian Embryonic Cell

Jones¹,

Elsdale²

1964

The Journal of Cell Biology

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Observations have been made of ultrastructural modifications induced in the nuclei of differentiating amphibian embryonic cells cultured in the presence of Actinomycin D. Of particular interest are regions within the nucleus (regions otherwise rather empty) containing loose groupings of uniform threads having a diameter of around 200 A. These threads have been observed in continuous lengths up to 0.5 #, and appear to be composed of subfilaments.

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Effect of Actinomycin D on Cellular Nucleic Acid Synthesis and Virus Production

Cited by 573 publications

References 7 publications

The induction of tumour cell adhesiveness and intercellular junctions by a glycoprotein of rat ascites hepatoma cell surface

The induction of tumour cell adhesiveness and intercellular junctions by a glycoprotein of rat ascites hepatoma cell surface

Lactate dehydrogenase enhances immunoglobulin production by human hybridoma and human peripheral blood lymphocytes

The Effects of Actinomycin D on the Ultrastructure of the Nucleus of the Amphibian Embryonic Cell

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