Effect of acidic solutions on the microhardness of dentin and set OrthoMTA and their cytotoxicity on murine macrophage

Abstract

ObjectivesTo evaluate the effects of three acids on the microhardness of set mineral trioxide aggregate (MTA) and root dentin, and cytotoxicity on murine macrophage.Materials and MethodsOrthoMTA (BioMTA) was mixed and packed into the human root dentin blocks of 1.5 mm diameter and 5 mm height. Four groups, each of ten roots, were exposed to 10% citric acid (CA), 5% glycolic acid (GA), 17% ethylenediaminetetraacetic acid (EDTA), and saline for five minutes after setting of the OrthoMTA. Vickers surface microhardness of set MTA and dentin was measured before and after exposure to solutions, and compared between groups using one-way ANOVA with Tukey test. The microhardness value of each group was analyzed using student t test. Acid-treated OrthoMTA and dentin was examined by scanning electron microscope (SEM). Cell viability of tested solutions was assessed using WST-8 assay and murine macrophage.ResultsThree test solutions reduced microhardness of dentin. 17% EDTA demonstrated severe dentinal erosion, significantly reduced the dentinal microhardness compared to 10% CA (p = 0.034) or 5% GA (p = 0.006). 10% CA or 5% GA significantly reduced the surface microhardness of set MTA compared to 17% EDTA and saline (p < 0.001). Acid-treated OrthoMTA demonstrated microporous structure with destruction of globular crystal. EDTA exhibited significantly more cellular toxicity than the other acidic solutions at diluted concentrations (0.2, 0.5, 1.0%).ConclusionsTested acidic solutions reduced microhardness of root dentin. Five minute's application of 10% CA and 5% GA significantly reduced the microhardness of set OrthoMTA with lower cellular cytotoxicity compared to 17% EDTA.

The effects of sodium hypochlorite and ethylenediaminetetraacetic acid on the microhardness of Mineral Trioxide Aggregate and TotalFill Bioceramic Putty

“…Depending on the ions that are available from the environment, fluids could penetrate into MTA, replacing any lost hydrates or calcium hydroxide . The calcium‐depleting and acidic nature of EDTA would produce a poor‐to‐non‐existent crystalline structure and a low calcium‐to‐silicate molar ratio . As EDTA affect the setting reactions of MTA, it should be flushed from the canals and chamber before this material is placed into position.…”

“…The microhardness of MTA is reduced by both NaOCl and EDTA, with EDTA being the most detrimental irrigant . Shoejee et al .…”

Push-Out Bond Strength and Surface Microhardness of Calcium Silicate-Based Biomaterials: An in vitro Study

“…Surface microhardness was measured using an automatic Vickers microhardness tester (FM-ARS 9000; Future-Tech Corp, Kawasaki, Japan). Five indents were made on the surface of each sample with a 25-g load applied for 5 s [18] . Values were recorded as Vickers hardness (HV) in an MS Excel spreadsheet.…”

Carbohydrate-electrolyte drinks exhibit risks for human enamel surface loss

“…TA and the ability of the drink to resist a change of pH, represented by buffering effect, are considered a better indicator of erosive potential than pH 31. Special attentions for β at 4.5 - 5.5, critical range pH, where enamel apatite dissolves without any mutual formation of surface fluorohydroxyapatite 2932. The higher the buffering effect of the drink, the more apatite will be dissolved before neutral pH can be reached.…”