Effect of acetylcholine on the secretion of gut glucagon immunoreactivity and gut glucagon-like immunoreactivity in pancreatectomized dogs.

Yoshida, Toshihide; Kondo, Motoharu

doi:10.1507/endocrj1954.27.33

Cited by 11 publications

(1 citation statement)

References 6 publications

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“…GLP secretion was slightly augmented by nerve stimulation during ␣-adrenergic blockade. In addition, acetylcholine weakly but significantly stimulated secretion, in agreement with previous investigations performed in vivo and in other species, suggesting that acetylcholine may stimulate L cell secretion (16,34,44). Thus it appears that cholinergic mechanisms may lead to a weak stimulation of GLP-1 secretion.…”

Section: E1015supporting

confidence: 90%

Somatostatin restrains the secretion of glucagon-like peptide-1 and -2 from isolated perfused porcine ileum

Hansen

Hartmann

Bisgaard

et al. 2000

American Journal of Physiology-Endocrinology and Metabolism

132

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Suspecting that paracrine inhibition might influence neuronal regulation of the endocrine L cells, we studied the role of somatostatin (SS) in the regulation of the secretion of the proglucagon-derived hormones glucagon-like peptide-1 and -2 (GLP-1 and GLP-2). This was examined using the isolated perfused porcine ileum stimulated with acetylcholine (ACh, 10(-6) M), neuromedin C (NC, 10(-8) M), and electrical nerve stimulation (NS) with or without alpha-adrenergic blockade (phentolamine 10(-5) M), and perfusion with a high-affinity monoclonal antibody against SS. ACh and NC significantly increased GLP secretion, whereas NS had little effect. SS immunoneutralization increased GLP secretion eight- to ninefold but had little influence on the GLP responses to ACh, NC, and NS. Basal SS secretion (mainly SS28) was unaffected by NS alone. Phentolamine + NS and NC abstract strongly stimulated release mainly of SS14, whereas ACh had little effect. Infused intravascularly, SS14 weakly and SS28 strongly inhibited GLP secretion. We conclude that GLP secretion is tonically inhibited by a local release of SS28 from epithelial paracrine cells, whereas SS14, supposedly derived from enteric neurons, only weakly influences GLP secretion.

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Section: E1015supporting

confidence: 90%