Effect of acetone feeding on alcohol dehydrogenase activity in the olive fruit fly, Bactrocera oleae

Abstract

The purpose of this study is to demonstrate a clear connection between the presence of acetone in larval diet and alcohol dehydrogenase (ADH) activity in laboratory raised populations of Bactrocera oleae. ADH activity of B. oleae is depressed in acetone-impregnated diets. At the same time the change of activity is accompanied by a change in the relative proportions of the multiple forms of ADH. The bulk of activity in the most cathodally migrating form is lost, and all the activity becomes localized in the less cathodally migrating forms of the enzyme. Moreover, ADH activity, expressed in vivo, appears to drop after exposure to ace-

Methoprene treatment reduces the pre‐copulatory period in Anastrepha fraterculus (Diptera: Tephritidae) sterile males

“…Contamination can be ruled out, because a different tip was used for pure acetone and methoprene solutions. We have no records of such effect in other Anastrepha ; however, in another tephritid fly, Bactrocera oleae (Gmelin), acetone exposure prolonged immature developmental time (egg to adult) (Cosmidis et al. 2002).…”

“…Contamination can be ruled out, because a different tip was used for pure acetone and methoprene solutions. We have no records of such effect in other Anastrepha; however, in another tephritid fly, Bactrocera oleae (Gmelin), acetone exposure prolonged immature developmental time (egg to adult) (Cosmidis et al 2002). The fact that acetone exposure was attained in Cosmidis et al (2002) by adding it to larval artificial medium makes any direct comparison difficult, but shows that acetone can affect development in a closerelated species.…”

“…2002). The fact that acetone exposure was attained in Cosmidis et al (2002) by adding it to larval artificial medium makes any direct comparison difficult, but shows that acetone can affect development in a close‐related species.…”

Adaptation Process of Wild Population of Olive Fruit Fly (Bactrocera Oleae (Rossi) (Diptera:Tepritidae)) into the Laboratory

“…Fruit Fly (Bactrocera Oleae (Rossi) (Diptera:Tepritidae)) into the Laboratory Emerged adults were formed F0 generation. They were placed in fine mesh screen adult rearing cages (30X30X30 cm in dimension) and provided with a solid diet containing hydrolyzed brewer's yeast, sugar, and dried egg yolk powder [19], [20], [21]. Water was also supplied by dental wicks in adult cages.…”

Possible Residual Effect of Pesticide onBactrocera oleaeRossı (Dıptera:Tephrıtıdae) Adults

“…Small scale rearing on artificial diets was reviewed by Silva (1970), Hagen (1966, Hagen et al (1963), Mittler & Tsitsipis (1973) and Genc (2008), and on host olive fruits by Tzanakakis (1971) and Genc & Nation (2008). Olive flies reared on an artificial diet differ from wild flies in certain aspects of their physiology, such as Allele frequencies (Economopolos 1980;Loukas et al 1985;Cosmidis et al 2002). The traditional control of this pest has been based on organophosphate insecticides for years in Turkey and many other countries.…”