Effect of acetochlor on transcription of genes associated with oxidative stress, apoptosis, immunotoxicity and endocrine disruption in the early life stage of zebrafish

Jiang, Jinhua; Wu, Shenggan; Liu, Xinju; Wang, Yanhua; An, Xingwei; Cai, Leiming; Zhao, Xueping

doi:10.1016/j.etap.2015.08.005

Cited by 69 publications

(30 citation statements)

References 62 publications

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“…These results imply that the pretilachlor can simultaneously induce endocrine disruption, oxidative stress increase and immunotoxicity during zebrafish embryo development. A number of studies have shown that bi-directional interactions connect oxidative stress with apoptosis and immunity [ 27 , 28 ], and apoptosis and oxidative stress affect cytokine responses of lymphocytes as well as apoptosis of immune cells [ 29 , 30 ]. However, no previous investigations to date have examined potential bi-directional interactions of oxidative stress, apoptosis and immune systems in response to rhein.…”

Section: Introductionmentioning

confidence: 99%

Rhein Induces Oxidative Stress and Apoptosis in Mouse Blastocysts and Has Immunotoxic Effects during Embryonic Development

Huang

Chan

2017

IJMS

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Rhein, a glucoside chemical compound found in a traditional Chinese medicine derived from the roots of rhubarb, induces cell apoptosis and is considered to have high potential as an antitumor drug. Several previous studies showed that rhein can inhibit cell proliferation and trigger mitochondria-related or endoplasmic reticulum (ER) stress-dependent apoptotic processes. However, the side effects of rhein on pre- and post-implantation embryonic development remain unclear. Here, we show that rhein has cytotoxic effects on blastocyst-stage mouse embryos and induces oxidative stress and immunotoxicity in mouse fetuses. Blastocysts incubated with 5–20 μM rhein showed significant cell apoptosis, as well as decreases in their inner cell mass cell numbers and total cell numbers. An in vitro development assay showed that rhein affected the developmental potentials of both pre- and post-implantation embryos. Incubation of blastocysts with 5–20 μM rhein was associated with increased resorption of post-implantation embryos and decreased fetal weight in an embryo transfer assay. Importantly, in an in vivo model, intravenous injection of dams with rhein (1, 3, and 5 mg/kg body weight/day) for four days resulted in apoptosis of blastocyst-stage embryos, early embryonic developmental injury, and decreased fetal weight. Intravenous injection of dams with 5 mg/kg body weight/day rhein significantly increased the total reactive oxygen species (ROS) content of fetuses and the transcription levels of antioxidant proteins in fetal livers. Additional work showed that rhein induced apoptosis through ROS generation, and that prevention of apoptotic processes effectively rescued the rhein-induced injury effects on embryonic development. Finally, the transcription levels of the innate-immunity related genes, CXCL1, IL-1 β and IL-8, were down-regulated in the fetuses of dams that received intravenous injections of rhein. These results collectively show that rhein has the potential to induce embryonic cytotoxicity and induce oxidative stress and immunotoxicity during the development of mouse embryos.

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Section: Introductionmentioning

confidence: 99%

Rhein Induces Oxidative Stress and Apoptosis in Mouse Blastocysts and Has Immunotoxic Effects during Embryonic Development

Huang

Chan

2017

IJMS

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“…These results collectively indicate that pretilachlor and rhein have the potential to cause embryonic cytotoxicity and induce oxidative stress and immunotoxicity during zebrafish or mouse embryo development. Several earlier studies have highlighted bi‐directional interactions and associations of oxidative stress with apoptosis and immunity and demonstrated that apoptotic processes and oxidative stress affect cytokine responses and production of lymphocytes as well as apoptosis of immune cells . However, until now, no related investigations have focused on potential bi‐directional interactions among oxidative stress, apoptosis and immune systems in response to ENN B1.…”

Section: Discussionmentioning

confidence: 99%

“…Accumulating evidence has highlighted the existence of bidirectional interactions involving oxidative stress, apoptosis and the immune system . Oxidative stress and cellular apoptosis clearly affect the cytokine response of lymphocytes and immune cells .…”

Section: Introductionmentioning

confidence: 99%

Enniatin B1 exerts embryotoxic effects on mouse blastocysts and induces oxidative stress and immunotoxicity during embryo development

Huang

Wang

Chan

2018

Environmental Toxicology

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“…This effect was assumed to be a feedback mechanism compensating for excess estrogenic signaling, consequently resulting in reduced VTG levels. Exposure to acetochlor resulted in an up‐regulation of aromatase and vtg gene expression in zebrafish larvae, whereas a down‐regulation of the estrogen receptor ß1 ( esr1b ) was observed (Jiang et al ). These authors thus claimed estrogenic activity for acetochlor, as similar effects of other estrogenic substances were described earlier, suggesting a feedback mechanism in the decreased estrogen receptor expression, compensating for the increased estrogenic activity.…”

Section: Cross‐talk Among 3 Endocrine Axes In Nonmammalian Vertebratesmentioning

confidence: 99%

“…Estrogen receptor activation leads directly to an increase in aromatase (i.e., cyp19a1b ) and vtg gene transcription, likely induced by increased estrogen receptor activation through an estrogen‐receptor–responsive element in their promoter regions (Pellegrini et al ). In consequence, a down‐regulation of esr1ß gene expression as a compensatory mechanism (Jiang et al ) is described, which finally results in down‐regulation of aromatase activity. Decreased aromatase activity results in masculinization, which has been described by Muth‐Kohne et al ().…”

Section: Cross‐talk Among 3 Endocrine Axes In Nonmammalian Vertebratesmentioning

confidence: 99%

Knotting nets: Molecular junctions of interconnecting endocrine axes identified by application of the adverse outcome pathway concept

Brüggemann

Licht

Fetter

et al. 2017

Enviro Toxic and Chemistry

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To be defined as an endocrine disruptor, a substance has to meet several criteria, including the induction of specific adverse effects, a specific endocrine mode of action, and a plausible link between both. The latter criterion in particular might not always be unequivocally determined, especially because the endocrine system consists of diverse endocrine axes. The axes closely interact with each other, and manipulation of one triggers effects on the other. The present review aimed to identify some of the many interconnections between these axes. The focus was on fish, but data obtained in studies on amphibians and mammals were considered if they assisted in closing data gaps, because most of the endocrine mechanisms are evolutionarily conserved. The review includes data both from ecotoxicological studies and on physiological processes and gives information on hormone/hormone receptor interactions or gene transcription regulation. The key events and key event relationships identified provide explanations for unexpected effects on one axis, exerted by substances suspected to act specifically on another axis. Based on these data, several adverse outcome pathway (AOP) segments are identified, describing connections between the hypothalamic-pituitary-gonadal (HPG) and hypothalamic-pituitary-thyroid (HPT) axes, the HPG and hypothalamic-pituitary-adrenal/interrenal (HPA/I) axes, and the HPT and HPA/I axes. Central key events identified across axes were altered aromatase activity as well as altered expression and function of the proteins 11b-hydroxysteroid dehydrogenase (11b-HSD) and steroidogenic acute regulatory (StAR) protein. Substance classes that act on more than one endocrine axis were, for example, goitrogens or aromatase inhibitors. Despite the wealth of information gathered, the present review only provides a few insights into the molecular nets of endocrine axes, demonstrating the complexity of their interconnections.

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Effect of acetochlor on transcription of genes associated with oxidative stress, apoptosis, immunotoxicity and endocrine disruption in the early life stage of zebrafish

Cited by 69 publications

References 62 publications

Rhein Induces Oxidative Stress and Apoptosis in Mouse Blastocysts and Has Immunotoxic Effects during Embryonic Development

Rhein Induces Oxidative Stress and Apoptosis in Mouse Blastocysts and Has Immunotoxic Effects during Embryonic Development

Enniatin B1 exerts embryotoxic effects on mouse blastocysts and induces oxidative stress and immunotoxicity during embryo development

Knotting nets: Molecular junctions of interconnecting endocrine axes identified by application of the adverse outcome pathway concept

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