Effect of Acetazolamide on the Chloride Shift and the Sodium Pump in Secretory Cells

Activated L-Arginine/Nitric Oxide Pathway in Pediatric Cystic Fibrosis and Its Association with Pancreatic Insufficiency, Liver Involvement and Nourishment: An Overview and New Results

“…This observation may suggest that both the plasma and the urinary concentrations of nitrate and nitrite are primarily dependent upon the reabsorption of nitrite by carbonic anhydrase (CA) in the proximal tubule of the nephron [ 47 ] ( Figure 1 ). By using the drug acetazolamide, a strong inhibitor of CA activity, early work found that CA is involved in CF [ 60 , 61 , 62 ]. Acetazolamide administration to cats was found to reduce bicarbonate concentration and to increase the concentration of chloride in the sweat [ 62 ].…”

“…By using the drug acetazolamide, a strong inhibitor of CA activity, early work found that CA is involved in CF [ 60 , 61 , 62 ]. Acetazolamide administration to cats was found to reduce bicarbonate concentration and to increase the concentration of chloride in the sweat [ 62 ]. In the meantime, there is strong evidence that CFTR and CA are co-expressed in various cells and organs, such as pancreatic and bile duct epithelial cells, including several nephron segments of mammalian kidney, mediate the bicarbonate transport, and are involved in the transport of other anions and cations, including Na + and K + by other transporters [ 63 , 64 , 65 , 66 , 67 , 68 ].…”

The origin and secretion of pancreatic juice bicarbonate

“…In the perfused cat pancreas, acetazolamide inhibits pancreatic secretion, just as it does in the intact gland (Case et al 1969a). Although acetazolamide is rather a specific carbonic anhydrase inhibitor, it can affect sodium secretion indirectly, perhaps by changing the intracellular pH (Slegers & Moons, 1968). Furthermore, there have been suggestions (Parsons, 1956;Kinney & Code, 1964;Kitahara, & Imamura, 1966) that acetazolamide may inhibit chloride transport by mechanisms other than interference with carbonic anhydrase.…”

Cation Transport and Cochlear Function

“…During the subsequent 17 minutes only a very slight decrease to 90 ( с was observed. The resemblance between the effects of COj inhalation and of acetazola mide perfusion may be due to an increase of intracellular CO¡, resulting in cellular acidosis in both cases (Siegers and Moons, 1968). The resulting decrease in intracellular pH could inhibit the cation pump, since the Na + -K + -ATPase system in the stria vascularis has a pH optimum of 7.3 and its activity falls considerably at lower pH (Fig.…”