Effect of acetate-free biofiltration and bicarbonate hemodialysis on neutrophil activation

Todeschini, Marta; Macconi, Daniela; García-Fernández, Núria; Ghilardi, Marina; Anabaya, Agostina; Binda, Elena; Morigi, Marina; Cattaneo, Dario; Perticucci, Elena; Remuzzi, Giuseppe; Noris, Marina

doi:10.1053/ajkd.2002.35690

Cited by 65 publications

(37 citation statements)

References 43 publications

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“…Production of CXCL12 and CXCL12 in the blood filtrate is most likely dependent on the activation of neutrophils, which are induced on the semipermeable hemofilter membranes used for hemofiltration of patients with chronic renal failure [26]. In stem cell mobilization, granulocytes are activated by G-CSF application, chemotherapy, or irradiation, which convert the BM in a highly proteolytic environment [3,27,28].…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Circulating Variants of CXCL12 from Human Plasma: Effects on Chemotaxis and Mobilization of Hematopoietic Stem and Progenitor Cells

Richter

Jochheim-Richter

Ciuculescu

et al. 2014

Stem Cells and Development

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Mobilization of hematopoietic stem and progenitor cells (HPCs) is induced by treatment with granulocytecolony stimulating factor, chemotherapy, or irradiation. We observed that these treatments are accompanied by a release of chemotactic activity into the blood. This plasma activity is derived from the bone marrow, liver, and spleen and acts on HPCs via the chemokine receptor CXCR4. A human blood peptide library was used to characterize CXCR4-activating compounds. We identified CXCL12 or CXCL12 induced a significant mobilization of HPCs in mice. Our findings indicate that plasma-derived CXCL12 variants may contribute to the regulation of HPC mobilization by modulating the binding of CXCL12 to GAGs rather than blocking the CXCR4 receptor and, therefore, may have a contributing role in HPC mobilization.

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Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Circulating Variants of CXCL12 from Human Plasma: Effects on Chemotaxis and Mobilization of Hematopoietic Stem and Progenitor Cells

Richter

Jochheim-Richter

Ciuculescu

et al. 2014

Stem Cells and Development

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“…Furthermore, acetate in concentrations present in regular bicarbonate dialysate is also reported to be proinflammatory, increasing cellular activation (7). It is possible that the reduced clotting with CD may be the result of the significantly lower concentration of acetate in CD compared with regular bicarbonate dialysate (0.3 mEq versus 4.0 to 8.0 mEq), thus promoting less clotting stimulation.…”

Section: Discussionmentioning

confidence: 99%

Increased Efficiency of Hemodialysis with Citrate Dialysate

Kossmann¹,

Gonzales

Callan

et al. 2009

Clinical Journal of the American Society of Nephrology

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Background and objectives: A bicarbonate dialysate acidified with citrate (CD) has been reported to have local anticoagulant effect. This study examines the effect of CD on dialysis efficiency, measured as eKt/Vurea, and predialysis concentrations of BUN, creatinine, phosphate, and ␤-2 microglobulin in chronic dialysis units.Design, settings, participants, & measurements: Three outpatient chronic hemodialysis units with 142 patients were switched to CD for 6 mo. Using each patient's prior 6 mo on regular bicarbonate dialysate acidified by acetate (AD) as control, eKt/Vurea was compared with that of CD. Follow-up data for 7 mo after the study were collected from about one-half of the participants remaining on CD and the others returned to AD.Results: eKt/Vurea, increased (P < 0.0001) from pre-CD value of 1.51 ؎ 0.01 to 1.57 ؎ 0.01 with CD. During CD use ␤-2 microglobulin levels declined (P ‫؍‬ 0.0001) from 28.1 ؎ 10.0 to 25.9 ؎ 10.0. Similarly, the concentrations of BUN, creatinine, and phosphate also decreased on CD (P < 0.008). In the poststudy period, eKt/Vurea for the patients staying on CD remained unchanged at 1.60 ؎ 0.17 versus 1.59 ؎ 0.18 (P ‫؍‬ NS), whereas in those returning to AD the eKt/Vurea decreased from 1.55 ؎ 0.20 to 1.52 ؎ 0.17 (P < 0.0001).Conclusions: Data suggest that CD use is associated with increased solute removal.

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“…In the 1990s, evidence accumulated that in bicarbonate dialysis, the small quantity of acetate present in the dialysis fluid as a pH stabilizing factor can lead to a significant transfer of that anion to the patient [1], inducing cytokine activation with production of nitric oxide, with potentially negative effects on uraemic cardiovascular disease [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

On-line haemodiafiltration with and without acetate

Pizzarelli

Cerrai

Dattolo

et al. 2006

Nephrology Dialysis Transplantation

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Background. In patients on on-line convective treatments, given the considerable quantity of dialysis fluid re-infused, the small amount of acetate present in bicarbonate dialysis fluid as a pH stabilizing factor may allow a significant transfer of that anion to the patient, possibly inducing cytokine activation. Methods. To verify this hypothesis, we performed on-line haemodiafiltration (OL-HDF) with (3 mmol/l) and without acetate in dialysis fluid in a cross-over randomized order on 12 prevalent patients. Results. In comparison with the pre-treatment values, plasma acetate levels were unchanged during and after acetate-free OL-HDF, while they were 5-6 times higher in the course of OL-HDF containing acetate in dialysis fluid; plasma acetate levels returned to basal values 2 h after the end of the procedure. The total increase of bases in the patient attributable to acetate was 36%.Plasma bicarbonate values at the end of treatment were significantly lower in treatments without acetate, as compared to those with acetate. Interleukin-6 plasma levels were super-imposable at the beginning and in the course of the two methods compared, but there was a tendency towards a greater increase at an interval of 2 h following OL-HDF with acetate. Conclusions. Our preliminary results confirm the assumption that body gain of acetate is particularly high in convective treatments, while acetate-free OL-HDF slows down acetate burden. Clinical advantages due to these effects should be evaluated in properly designed prospective studies.

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Effect of acetate-free biofiltration and bicarbonate hemodialysis on neutrophil activation

Cited by 65 publications

References 43 publications

Identification and Characterization of Circulating Variants of CXCL12 from Human Plasma: Effects on Chemotaxis and Mobilization of Hematopoietic Stem and Progenitor Cells

Identification and Characterization of Circulating Variants of CXCL12 from Human Plasma: Effects on Chemotaxis and Mobilization of Hematopoietic Stem and Progenitor Cells

Increased Efficiency of Hemodialysis with Citrate Dialysate

On-line haemodiafiltration with and without acetate

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