Effect of accelerated ageing on the viability and germination of European beech (Fagus sylvatica L.) seeds

Procházková, Z.; Bezděčková, L.

doi:10.15258/sst.2009.37.3.17

Cited by 4 publications

(5 citation statements)

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“…Such a result confirms that seedling emergence is a better measure of seed viability since it represents the actual number of regenerated plants. Seeds showing high germination but poor emergence are considered low vigor seeds having poor storage potential [ 13 , 53 ]. Significantly, TTC assay revealing desiccation induced a decline in respiratory activity at 10.5% of MC, which might be considered as a first sign representative of biochemical lesions [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, reliable up-to-date information about the quality of stored seeds is needed to indicate the time when seeds need to be delivered from warehouse to nurseries. Additionally, regarding the fact of inevitable aging of seeds, the preserved seed lots may vary considerably [ 9 , 13 ], and even if the optimal beech seed storage protocol is applied, there are seed lots that lose their high initial viability and deteriorate after few year of storage, whereas others retain their high viability. Measurement of the global DNA methylation level could be successfully used to predict potential viability of seeds of orthodox, recalcitrant, and intermediate categories regardless their composition (e.g., lipid-rich beech seeds vs. poplar seeds with active chloroplasts).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, often unpredictable weather events may affect seed production stronger than climatic, genetic, or environmental factors [ 10 , 12 ]. Thus, is seems clear that to ensure a sufficient number of seedlings for reforestation, seeds must be stored for a long time; hence, optimal protocols for their effective storage are needed [ 9 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…Importantly, the true values of germinability can only be assessed after a full stratification and germination test that lasts longer than 20 weeks [ 9 ]. However, it is important to notice that a germination test conducted in the laboratory under optimal conditions might not provide real information about the seed deterioration stage; thus, seeds showing few signs of aging might give poor germination results in the more stressful field conditions [ 13 ]. Moreover, because of the time consuming stratification-germination procedure, the data on seed quality can be obtained too late to be able to pretreat the seeds for sowing in spring, while cold storage until the following year is associated with a gradual decline in seed quality [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification of DNA Methylation Changes in European Beech Seeds during Desiccation and Storage

Michalak

Plitta-Michalak

Suszka

et al. 2023

IJMS

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Ageing and deterioration of seeds is a major problem for the maintenance of seed quality and viability during long-term storage. Prediction of early stages of seed deterioration in order to point out the plantlets’ regeneration time is a major challenge of successful storage. In preserved seeds, damages accumulate within cells at the rate mainly related to their moisture content and temperature of storage. Current research reveals global alterations in DNA methylation in lipid-rich intermediate seeds during desiccation and storage at various regimes covering nonoptimal and optimal conditions. We show for the first time that monitoring of 5-methylcytosine (m5C) level in seeds can be used as a truly universal viability marker regardless of postharvest category of seeds and their composition. For seeds stored up to three years, in varied conditions, moisture content, temperature, and time of storage had significant influence on seedling emergence and DNA methylation (p < 0.05). Similarities among lipid-rich intermediate and orthodox seeds regarding different reactions of embryonic axes and cotyledons to desiccation are newly revealed. Along with previous studies on seeds dramatically different in desiccation tolerance (recalcitrant vs. orthodox), results regarding lipid-rich seeds positioned in-between (intermediate) prove that maintaining global DNA methylation status is crucial for maintaining seed viability.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of DNA Methylation Changes in European Beech Seeds during Desiccation and Storage

Michalak

Plitta-Michalak

Suszka

et al. 2023

IJMS

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“…Seed aging is most often analyzed by applying various accelerated aging tests (Al‐Maskri et al, 2003; Prochazkova and Bezdeckova, 2009; Matthews et al, 2010; Ohlson et al, 2010; Eksi and Demir, 2011). The studies on a more natural long‐term aging of seed are rather scarce.…”

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confidence: 99%

Physiological and Biochemical Parameters of Lupin Seed Subjected to 29 Years of Storage

2017

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Physiological and biochemical changes resulting from long‐term storage of lupin (Lupinus luteus L.) seed were studied in a precisely controlled experiment to gain a better knowledge of the seed deterioration process. The seed was stored at different temperatures (0, −14, and +20°C) during a 29‐yr period. It was found that after long‐term storage at −14 and 0°C, the seed germinated well, whereas seed stored at +20°C did not germinate at all. The contents of albumin, biogenic amines, and soluble carbohydrates were determined. The content of albumin decreased in seed stored at +20°C. In two‐dimensional electrophoresis of seed proteins, 343 spots were obtained with seed stored at −14°C, whereas the extract of seed stored at +20°C gave 290 spots. Out of all detected spots, 27 displayed significant differences in intensity related to seed storage temperature (−14, 0, +20°C). The identified 27 proteins could be assigned to four major functional categories: storage proteins, proteins connected with defense mechanisms, proteins responsible for energy reactions, and proteins involved in growth and development. Spermine, spermidine, and putrescine were also analyzed in stored seed. The content of biogenic amines was higher in seed stored at +20°C. Significant changes occurred in the content of soluble carbohydrates during seed storage. In seed stored at +20°C, verbascose did not occur and the total content of raffinose family oligosaccharides decreased. The content of reducing monosaccharides and sucrose increased. The results show that lupin seed storage at −14 and 0°C resulted in very similar levels of physiological and biochemical changes, affecting the germination rate, proteome, biogenic amines, and soluble carbohydrates, whereas storage at +20°C is incomparably more detrimental.

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Effect of accelerated ageing on the viability and germination of European beech (Fagus sylvatica L.) seeds

Cited by 4 publications

References 0 publications

Identification of DNA Methylation Changes in European Beech Seeds during Desiccation and Storage

Identification of DNA Methylation Changes in European Beech Seeds during Desiccation and Storage

Physiological and Biochemical Parameters of Lupin Seed Subjected to 29 Years of Storage

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