Effect of abiotic stresses on glutathione peroxidase and glutathione S-transferase activity in barley root tips

Halušková, Ľubica; Valentovičová, Katarína; Huttová, Jana; Mistrı́k, Igor; Tamás, Ladislav

doi:10.1016/j.plaphy.2009.08.003

Cited by 105 publications

(42 citation statements)

References 43 publications

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“…3e & f). Increased GPX and GST activity due to Cd stress were also reported in tomato (Gratao et al 2008), in rice seedlings (Hu et al 2009), in Arabidopsis (Semane et al 2007;Smeets et al 2008), in barley root tips (Haluskova et al 2009) and in Brassica napus (Nouairi et al 2009). In the present investigation, the increases of GST and GPX were not high enough to protect cells from Cd-induced oxidative damage.…”

Section: Discussionmentioning

confidence: 73%

“…For Cd stress, seedlings were treated with Hyponex solution that contained 1 mM CdCl 2 in Petri plates lined with 4 layers of filter paper under the above conditions for 48 h. For Cd treatment in the presence of proline or betaine, seedlings were treated with 5 mM proline or 5 mM betaine with the above levels of CdCl 2 in Hyponex solution for 48 h. Control plants were grown in Hyponex solution only. In case of heavy metals, due to high binding capacity of filter paper to cations (Haluskova et al 2009;Tamás et al 2008;Kuriakose and Prasad 2008), application of 1 mM CdCl 2 concentration was required to obtain meaningful differences among the metabolites and the enzymatic activities within short period of time (48 h). Since the primary objectives of this investigation was to study the response of seedlings to Cd toxicity as well as biochemical mechanisms of proline-and betaine-induced Cd stress tolerance, these high concentrations were used throughout the experiment.…”

Section: Plant Materials and Stress Treatmentsmentioning

confidence: 99%

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Up-regulation of antioxidant and glyoxalase systems by exogenous glycinebetaine and proline in mung bean confer tolerance to cadmium stress

Hossain

Hasanuzzaman

Fujita

2010

Physiol Mol Biol Plants

365

162

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Section: Discussionmentioning

confidence: 73%

Section: Plant Materials and Stress Treatmentsmentioning

confidence: 99%

Up-regulation of antioxidant and glyoxalase systems by exogenous glycinebetaine and proline in mung bean confer tolerance to cadmium stress

Hossain

Hasanuzzaman

Fujita

2010

Physiol Mol Biol Plants

365

162

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“…Increased GR activity in the heat pretreated salt and drought-stressed seedlings contributes to the maintenance of higher GSH/GSSG ratio and GSH level which is used by DHAR and other GSH-dependent enzymes involved in the ROS and MG detoxification systems [7,[26][27][28]52]. The potential role of GST isozymes has been extensively studied in plants due to its well-defined role on plant detoxification reactions [94][95]. Various abiotic stress effects are powerful inducers of GST activity in plants [96][97][98][99][100][101].…”

Section: Discussionmentioning

confidence: 99%

Heat-shock positively modulates oxidative protection of salt and drought-stressed mustard (Brassica campestris L.) seedlings

Hossain¹,

Mostofa²,

Fujita³

2013

J Plant Sci Mol Breed

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ResearchOpen AccessHeat-shock positively modulates oxidative protection of salt and drought-stressed mustard (Brassica campestris L.) seedlings AbstractA large number of studies have shown the existence of cross-tolerance in plants, but the exact physiological and biochemical mechanism(s) is poorly understood. In this study, heat-shock (42 °C, 5 h) induced salinity and drought tolerance and possible involvement of antioxidative and glyoxalase systems were investigated in mustard (Brassica campestris L.) seedlings. Seven-day-old seedlings were subjected to salt (150 mM NaCl, 48 h) and drought stress (induced by 20% PEG, 48 h) with or without heat pretreatment. Both salt and drought stresses led to a severe oxidative stress as indicated by profound increases in hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) levels. A significant increase in ascorbate (AsA) content was observed in response to drought stress but the glutathione (GSH) and glutathione disulfide (GSSG) contents increased in response to both salt and drought stress. The GSH/GSSG ratio decreased significantly in response to drought stress. Salt stress led to a significant increases of ascorbate peroxidase (APX), glutathione reductase (GR), glutathione S-transferases (GST) activities; whereas, catalase (CAT) and glyoxalase II (Gly II) activities decreased. Drought stress resulted in a significant increase in monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione peroxidase (GPX) and glyoxalase I (Gly I) activities; whereas, CAT and Gly II activities decreased. Seedlings primed with heat-shock positively modulates the activities of APX, DHAR, GR, GST, GPX, CAT, Gly I and Gly II, and maintained lower levels of GSSG, H 2 O 2 and MDA as compared to the control mostly also salt and drought-stressed seedlings. Our results showed that a retention of the imprint of previous stress exposure (heat-shock) protects the plants from salt and drought-induced oxidative stress by stimulation of antioxidative and glyoxalase defense systems.

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“…Peroxidases are not only constitutive but also inducible enzymes, which are synthesized under the influence of various physical, chemical, and biotic agents (Haluskova et al 2009). Their molecular and functional heterogeneity makes it possible to change the ratio between isoforms in favor of these more adapted to adverse environmental conditions, and this increase in peroxidase activity may be considered as defense function of the plant organism (Morkunas & Gmerek 2007).…”

Section: Discussionmentioning

confidence: 99%

Salicylic acid increases the defense reaction against bunt and smut pathogens in wheat calli

Максимов

Troshina

Surina

et al. 2013

Journal of Plant Interactions

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The role of salicylic acid (SA) in regulating wheat calli growth and peroxidase activity in the co-culture with bunt (Tilletia caries (D.C.) Tul.) and smut (Ustilago tritici Pers.) pathogens was studied in this work. We found that the influence of SA increased the number of globular high-density sites with meristema-like cells and reduced the number of low-structured sites with parenchyma-like cells in wheat calli. SA inhibited fungi spores germination, mycelium growth, and formation of its new spores during the co-cultivation of wheat calli with bunt or smut agent. Interaction of fungi mycelium with wheat calli treated by SA led to formation of zones with hypersensitive reaction in calli high-structured sites with meristema-like cells. The obtained data about the influence of SA on activation of wheat calli growth and the peroxidases with pI Â3.5 and Â9.8, which can bind to the mycelium of T. caries or U. tritici are discussed in this paper.

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Effect of abiotic stresses on glutathione peroxidase and glutathione S-transferase activity in barley root tips

Cited by 105 publications

References 43 publications

Up-regulation of antioxidant and glyoxalase systems by exogenous glycinebetaine and proline in mung bean confer tolerance to cadmium stress

Up-regulation of antioxidant and glyoxalase systems by exogenous glycinebetaine and proline in mung bean confer tolerance to cadmium stress

Heat-shock positively modulates oxidative protection of salt and drought-stressed mustard (Brassica campestris L.) seedlings

Salicylic acid increases the defense reaction against bunt and smut pathogens in wheat calli

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