Effect of ABCE1-silencing gene, transfected by electrotransfer, on the proliferation, invasion, and migration of human thyroid carcinoma SW579 cells

Qu, Xin-Ping; Zhang, L

doi:10.4238/2015.november.18.32

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…Based on the functions of the ABCE1 gene, a previous study suggested that the ABCE1 gene is associated with tumor formation, prognosis and metastasis (7). It has been demonstrated that when the ABCE1-silencing gene is transfected into tumor cells, there is a substantial change in their proliferation and apoptosis (33). A previous study revealed that the expression levels of the ABCE1 protein in lung carcinoma tissues and metastatic lymph nodes are higher compared with those in normal tissues (7).…”

Section: Discussionmentioning

confidence: 99%

Tip60‑siRNA regulates ABCE1 acetylation to suppress lung cancer growth via activation of the apoptotic signaling pathway

Liang

et al. 2019

Exp Ther Med

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Lung cancer is a leading cause of cancer-associated mortality and morbidity worldwide. Previous studies have suggested that ATP-binding cassette transporter E1 (ABCE1) acetylation is upregulated in the tissues and cells of lung cancer and is associated with the prognosis of patients with lung cancer. The aim of the present study was to investigate the association between Tat interactive protein 60 kDa (Tip60) expression and ABCE1 acetylation, and the effect of Tip60 on the biological functions of A549 lung carcinoma cells. The expression levels of Tip60 and ABCE1 acetylation were examined using western blot and co-immunoprecipitation (Co-IP) assays in normal bronchial epithelial (HBE) and human lung cancer (A549) cells. The expression of Tip60 then was downregulated in A549 cells using small interfering RNA. Wound healing and Transwell assays were used to assess cell invasion and migration. The biological effects of Tip60 in lung cancer cells were investigated using MTT and flow cytometric assays. Subsequently, tumor xenografts were established to observe the effect of Tip60 on lung cancer in vivo. Western blot and Co-IP assays were performed to investigate the mechanism of Tip60 in A549 cells. Tip60 expression and ABCE1 acetylation were upregulated in the lung cancer cells compared with the normal bronchial epithelial cells. Downregulation of Tip60 decreased the acetylation of ABCE1 and inhibited cell proliferation, invasion and migration. Furthermore, the downregulation of Tip60 activated the apoptotic pathway in order to achieve its suppressive function. In the xenografts, the tumor weight and volume were notably reduced due to the downregulation of Tip60 expression. The results of the present study strongly suggest that Tip60 is a novel target in the prevention and treatment of lung cancer.

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Section: Discussionmentioning

confidence: 99%

Tip60‑siRNA regulates ABCE1 acetylation to suppress lung cancer growth via activation of the apoptotic signaling pathway

Liang

et al. 2019

Exp Ther Med

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“…Huang et al [29] reported that ABCE1 deletion could induce apoptosis and inhibit proliferation and migration of esophageal cancer cells. In thyroid cancer, ABCE1 was demonstrated to enhance cell viability and invasion in vitro [17]. Notably, ABCE1 has also been found involved in the development of chemotherapeutic resistance in cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…These members are further divided into seven subfamilies designated as ABC A–G [16]. Recently, a growing body of evidence has indicated that ABC E1 (ABCE1), a member of the ABC family, is associated with some biological behaviors of cancer cells, such as proliferation, migration, and invasion [17,18]. Furthermore, ABCE1 has been reported to be abnormally expressed in a variety of drug-resistant cell types, suggesting its certain effect on chemoresistance [19].…”

Section: Introductionmentioning

confidence: 99%

Down-regulation of ABCE1 inhibits temozolomide resistance in glioma through the PI3K/Akt/NF-κB signaling pathway

et al. 2018

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The ATP binding cassette (ABC) E1 (ABCE1), a member of the ABC family, was originally described as the RNase L inhibitor. Through forming a heterodimer with RNase L, ABCE1 participates in the negative regulation of the 2-5A/RNase L system and thus mediates a wide range of biological functions. Recent evidence has shown the new roles of ABCE1 in tumorigenesis. However, there have been no investigations on the specific effect of ABCE1 on glioma. In the present study, we examined the expression pattern and possible role of ABCE1 in glioma. Our study demonstrated that ABCE1 was up-regulated in glioma tissues and cell lines. Down-regulation of ABCE1 inhibited temozolomide (TMZ) resistance of glioma cells in vitro and in vivo. In addition, we found that the PI3K/Akt/NF-κB pathway was involved in ABCE1-mediated chemoresistance of glioma cells. Taken together, our study suggested ABCE1 as a promising target for glioma chemotherapy.

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Diagnosis of thymic epithelial tumor subtypes by a quantitative proteomic approach

Zhao

Liu

et al. 2018

Analyst

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The histological typing of thymic epithelial tumours (TETs) still remains a challenge for surgical pathologists, especially when encountering borderline cases mainly focused on spindle cell types (including type A, atypical type A (aA), AB, and B3). A systematic proteomics analysis of TETs was performed using isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS). In total, 6479 and 6305 proteins were identified and quantified, respectively. After Gene Ontology (GO) annotation and Ingenuity Pathway Analysis (IPA), six differentially expressed proteins were validated by tissue microarray or multiple reaction monitoring (MRM) quantification. ABCE1 and CLIC2 are promising to be diagnostic candidate biomarkers in thymic carcinomas (TCs). CHD1L was up-regulated in type AB and type B thymomas compared with type A thymoma. Both CLIC2 and MAP7 were negatively detected in type B1 and B2 thymomas. SMAD4 was overexpressed in type aA thymomas and TCs. CDC42 was significantly down-regulated in type B2 thymomas compared with other subtypes. Six novel candidate biomarkers were found to be useful in differentiating subtypes of TETs. SMAD4 may play a specific role in tumorigenesis and the development of aA thymomas and thymic carcinomas.

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Effect of ABCE1-silencing gene, transfected by electrotransfer, on the proliferation, invasion, and migration of human thyroid carcinoma SW579 cells

Cited by 4 publications

References 12 publications

Tip60‑siRNA regulates ABCE1 acetylation to suppress lung cancer growth via activation of the apoptotic signaling pathway

Tip60‑siRNA regulates ABCE1 acetylation to suppress lung cancer growth via activation of the apoptotic signaling pathway

Down-regulation of ABCE1 inhibits temozolomide resistance in glioma through the PI3K/Akt/NF-κB signaling pathway

Diagnosis of thymic epithelial tumor subtypes by a quantitative proteomic approach

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