Effect of a Velogenic Newcastle Disease Virus on Testosterone Concentration and Gonadal Sperm Reserves of Vaccinated Shikabrown Cocks

Ruwaan, J.S.; Rekwot, P.I.; Abdu, PA; Omontese, B. O.; Obidi, J.A.; Chiezey, N. P.

doi:10.3923/rjpscience.2010.48.53

Cited by 1 publication

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“…Vitamin D3, E 2 , and testosterone levels were dosed in the epididymal region and plasma by ELISA assays (Akhlaghi & Zamiri 2007, Ruwaan et al 2010. For tissue analysis, epididymal region of affected and non-affected animals (nZ5) were frozen in liquid nitrogen and macerated in dry ice.…”

Section: Hormone Measurementsmentioning

confidence: 99%

Roosters affected by epididymal lithiasis present local alteration in vitamin D3, testosterone and estradiol levels as well as estrogen receptor 2 (β) expression

Oliveira

Dornas²,

Praes³

et al. 2011

Reproduction

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Epididymal lithiasis is a reproductive dysfunction of roosters that is associated with loss of fertility and is characterized by the formation of calcium stones in the lumen of the efferent ductules of the epididymal region. The efferent ductules of birds are responsible for the reabsorption of the fluid coming from the testis as well as luminal calcium. It has been hypothesized that the epididymal stone formation may be related to the impairment of local fluid or calcium homeostasis, which depends on hormones such as estradiol (E 2 ). Therefore, this study aimed to investigate possible alterations in the expression of ERa (ESR1) and ERb (ESR2) in the epididymal region of roosters affected by epididymal lithiasis. The study was performed by immunohistochemistry and western blotting assays. In addition, the concentrations of E 2 , vitamin D3, and testosterone, which are also key hormones in maintenance of calcium homeostasis, were determined in the plasma and epididymal region, by ELISA. It was observed that ESR2 expression is increased in all segments of the epididymal region of affected roosters, whereas ESR1 levels are not altered. Moreover, the hormone concentration profiles were changed, as in the epididymal region of roosters with lithiasis the E 2 levels were increased and vitamin D3 as well as testosterone concentrations were significantly decreased. These results suggest that a hormonal imbalance may be involved with the origin and progression of the epididymal lithiasis, possibly by affecting the local fluid or calcium homeostasis.

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Section: Hormone Measurementsmentioning

confidence: 99%