Effect of a Surfactant on the Antimicrobial Activity of Sodium Hypochlorite Solutions

Abstract

The objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (NaOCl) associated with a surfactant. Seventy single-rooted extracted human teeth were inoculated with Enterococcus faecalis, and incubated for 21 days (37 °C). The groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% NaOCl; 5%, 2.5% and 1% Hypoclean®, a solution containing a surfactant (cetrimide) associated with NaOCl. Three microbiological samples were collected from each tooth: S1 - before instrumentation; S2 - immediately after instrumentation; and S3 - after a seven-day period. Data were submitted to ANOVA and Tukey test with 5% significance level. The results showed that immediately after root canal preparation (S2), E. faecalis was eliminated in all the experimental groups. However, after 7 days (S3), only the groups in which Hypoclean was used, remained contamination-free, including Hypoclean associated with 1% NaOCl, while the root canals irrigated with 1% NaOCl only, presented the highest percentage of bacterial growth. In conclusion, the addition of surfactant increased the antimicrobial activity of 1% NaOCl to levels similar to 5% NaOCl.

Chemical Composition of Black Spruce (Picea mariana) Bark Extracts and Their Potential as Natural Disinfectant

“…48,49 In studies reported by Bolfoni et al and Perez-Conesa, Mclandsborough and Weiss, the addition of surfactants, such as surfynol, centrimide and polypropylene glycol, showed greater antimicrobial activity of the antimicrobial agent. 50,51 Overall, our results reveal interesting antimicrobial activity for OPF against E. coli. Effectiveness towards this bacterial strain demonstrated the potential of black spruce extracts, since this gram-negative bacteria is generally more resistant to antimicrobial agents than gram-positive bacteria.…”

Disinfection and surface changes of gutta‐percha cones after immersion in sodium hypochlorite solution containing surfactant

“…The authors tested different NaOCl solutions with and without cetrimide in the irrigation of 70 uniradicular human teeth contaminated by E. faecalis ; and observed that at the 7‐day period treatment, only the NaOCl associated with the surfactant‐treated specimens were bacterial contamination free. This result may be explained due to a possible residual effect of the surfactant molecule (Bolfoni et al, ). Cetrimide, a quaternary ammonium, has chemical activity similar to chlorhexidine, characterized by its substantivity (Bolfoni et al, ).…”

“…This result may be explained due to a possible residual effect of the surfactant molecule (Bolfoni et al, ). Cetrimide, a quaternary ammonium, has chemical activity similar to chlorhexidine, characterized by its substantivity (Bolfoni et al, ).…”

“…Cetrimide, a quaternary ammonium, has chemical activity similar to chlorhexidine, characterized by its substantivity (Bolfoni et al, 2014).…”

Antimicrobial effectiveness of combinations of oxidant and chelating agents in infected dentine: an ex vivo confocal laser scanning microscopy study

“…Previous studies, using microbiological culture (Bolfoni et al . ), turbidity of culture medium measured by a UV spectrophotometer (Giardino et al . ), viability staining and confocal microscopy (Wang et al .…”