Effect of a probiotic beverage consumption (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) in rats with chemically induced colitis

Abstract

BackgroundSome probiotic strains have the potential to assist in relieving the symptoms of inflammatory bowel disease. The impact of daily ingestion of a soy-based product fermented by Enterococcus faecium CRL 183 and Lactobacillus helveticus 416 with the addition of Bifidobacterium longum ATCC 15707 on chemically induced colitis has been investigated thereof within a period of 30 days.MethodsColitis was induced by dextran sulfate sodium. The animals were randomly assigned into five groups: Group C: negative control; Group CL: positive control; Group CLF: DSS with the fermented product; Group CLP: DSS with the non-fermented product (placebo); Group CLS: DSS with sulfasalazine. The following parameters were monitored: disease activity index, fecal microbial analyses, gastrointestinal survival of probiotic microorganisms and short-chain fatty acids concentration in the feces. At the end of the protocol the animals’ colons were removed so as to conduct a macroscopical and histopathological analysis, cytokines and nitrite quantification.ResultsAnimals belonging to the CLF group showed fewer symptoms of colitis during the induction period and a lower degree of inflammation and ulceration in their colon compared to the CL, CLS and CLP groups (p<0.05). The colon of the animals in groups CL and CLS presented severe crypt damage, which was absent in CLF and CLP groups. A significant increase in the population of Lactobacillus spp. and Bifidobacterium spp. at the end of the protocol was verified only in the CLF animals (p<0.05). This group also showed an increase in short-chain fatty acids (propionate and acetate). Furthermore, the intestinal survival of E. faecium CRL 183 and B. longum ATCC 15707 in the CLF group has been confirmed by biochemical and molecular analyzes.ConclusionsThe obtained results suggest that a regular intake of the probiotic product, and placebo to a lesser extent, can reduce the severity of DSS-induced colitis on rats.

A soy-based probiotic drink modulates the microbiota and reduces body weight gain in diet-induced obese mice

“…seems to be involved in obesity-associated dysbiosis (Ignacio et al, 2015;Koliada et al, 2017;Krajmalnik-Brown et al, 2012;Million et al, 2012;Sweeney & Morton, 2013). A previous study that evaluated the effect of the same probiotic drink on the development of DSS-induced colitis, found an increase in the fecal population of Bifidobacterium spp., and elevation in acetate and propionate levels in the colon of C57BL6 mice (Celiberto et al, 2017). The genus Bifidobacterium spp.…”

“…Probiotic and respective placebo products were obtained from UniverSojaa unit that develops and produces soy products at the Faculty of Pharmaceutical Sciences, UNESP-Araraquara, Brazil. The fermented product was obtained according to the methodology proposed by Rossi, Vendramini, Carlos, Pei, and de Valdez (1999), with modifications (Celiberto et al, 2017), using the starting cultures E. faecium CRL 183 (probiotic -1.5% v/v) and L. helveticus 416 (technological purposes -1.5% v/v). The fermentation was carried out at 37°C and when the product reached a pH of 4.5, the strain of B. longum ATCC 15707 (probiotic) was added in sufficient amount to achieve 8 logCFU ml −1 in the final product.…”

A Galacto-Oligosaccharides Preparation Derived From Lactulose Protects Against Colorectal Cancer Development in an Animal Model

“…At the level of the Actinobacteria phylum, GOS-Lu cohort shows an increase in this bacteria, mainly due to more abundant populations of Bifidobacteriaceae family, and specially of Bifidobacterium genus, being associated to health benefits including protection against pro-inflammatory gut conditions but also against CRC ( Table 1 and Figure 9 ). This protection against CRC has been associated to NF-kB repression by these probiotic bacteria ( Roller et al, 2004a ; Kim et al, 2010 ; Celiberto et al, 2017 ; Paveljšek et al, 2018 ).…”

A Network of Physiological Interactions Modulating GI Homeostasis: Probiotics, Inflammasome, mTOR

“…A fermented soy probiotic mixture was shown to provide multiple gastrointestinal health benefits to rats with induced colitis. Rats fed the probiotic mixture of Bifidobacterium longum and Lactobacillus helveticus 416 had no colon damage, ulcers, or swelling, compared to rats who did not receive the probiotic supplement [25]. The rats receiving the probiotic also showed increased intestinal Lactobacillus and Bifidobacterium populations [25].…”

“…Rats fed the probiotic mixture of Bifidobacterium longum and Lactobacillus helveticus 416 had no colon damage, ulcers, or swelling, compared to rats who did not receive the probiotic supplement [25]. The rats receiving the probiotic also showed increased intestinal Lactobacillus and Bifidobacterium populations [25]. Supplementation with probiotics can help adjust the gut microbiota, and this likely plays a role in the effects of diseases of the gut.…”