Effect of a Peracetic Acid-Based Disinfectant on Growth, Hematology and Histology of Juvenile Rainbow Trout (Oncorhynchus mykiss)

Hushangi, Ramtin; Shekarabi, Seyed Pezhman Hosseini

doi:10.3390/fishes3010010

Cited by 16 publications

(11 citation statements)

References 20 publications

(25 reference statements)

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“…Evidence of global molecular responses is lacking in our current understanding of the physiological consequences of PAA exposure in fish (Gesto, Liu, Pedersen, Meinelt, Straus, Jokumsen, 2018;Hushangi, Hosseini Shekarabi, 2018;Liu, Lazado, Pedersen, Straus, Meinelt, 2020). Here, we show that the transcriptome of the two mucosal tissues that directly interacted with PAA during treatment responded differently to PAA, with the gills exhibiting a stronger response than the skin (Figure 4).…”

Section: The Dynamics Of Mucosal Molecular Responses To Paa Are Differentially Affected By Crowding Historymentioning

confidence: 71%

Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid

et al. 2021

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Knowledge of the impact of aquaculture chemotherapeutants on fish physiology is scarce. This is particularly relevant for peracetic acid (PAA), a widely used oxidative disinfectant in aquaculture. The chemical behaviour in water is well studied but knowledge about the physiological consequences for fish is limited. The present study investigated the transcriptomics, morphology, and physiology of Atlantic salmon (Salmo salar) responses to PAA and explored how crowding prior to exposure influenced these responses. Post-smolts were subjected to crowding by reducing the water volume thereby increasing the density for 1 h before they were exposed to 4.8 ppm PAA for 30 minutes. The exposed fish were allowed to recover for 2 weeks (w), with samplings carried out at 4 h and 2 w postexposure (p.e.). There were four treatment groups in total: no crowding/control; no crowding/PAA; crowding/control; and crowding/PAA. The physiological changes were documented at the mucosal (i.e., skin and gills) and systemic (i.e., plasma) levels. The overall external welfare score was in good status in all experimental groups. The treatments did not dramatically affect the number of mucous cells in both the skin and the gills. Branchial histomorphology was in a fairly good condition, despite the increased occurrence of epithelial lifting in the crowded groups at 2 w p.e. The gill transcriptome was affected by crowding, PAA, and their combinations more than the skin, as manifested by the number of differentially expressed genes (DEG) in the former. In general, individual stimuli and their combinations elicited strong transcriptional responses in the gills at 4 h p.e. and a marked recovery was observed 2 w thereafter. Crowding altered the dynamics of transcriptional response to PAA especially at 4 h p.e. and the two mucosal tissues demonstrated a contrasting profilea higher number of DEGs in the gills without crowding history, while higher skin DEGs were observed in the group subjected to crowding prior to exposure. Plasma metabolomics identified 639 compounds, and the metabolomic changes were affected mainly by crowding and sampling time, and not by PAA exposure. The results revealed the ability of salmon to mobilise physiological countermeasures to PAA exposure that were differentially influenced by crowding, and that such an effect was remarkably exhibited at the mucosa rather than in the circulating metabolome.

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Section: The Dynamics Of Mucosal Molecular Responses To Paa Are Differentially Affected By Crowding Historymentioning

confidence: 71%

Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid

et al. 2021

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“…At the end of the trial, five fish were randomly selected from each aquarium and after anaesthetization by clove powder (150 ppm), blood samples were collected from the caudal vein. Haematological parameters such as white blood cells (WBCs), red blood cells (RBCs), haematocrit or packed cell volume (PCV), and haemoglobin (Hb) were measured (Hushangi & Hosseini Shekarabi, ). For serum biochemical analysis, the blood samples were centrifuged at 2,600 × g (at 4°C, for 10 min), and then cholesterol (CHOL), glucose, superoxide dismutase (SOD), alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) contents were measured using a biochemical autoanalyzer (Prestige 24i, Tokyo Boeki Medical System) by commercial test kits according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

Determination of grapefruit (Citrus paradisi) peel extract bio‐active substances and its application in Caspian white fish (Rutilus frisii kutum) diet: Growth, haemato‐biochemical parameters and intestinal morphology

et al. 2019

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In this study, total phenolic and flavonoid contents of grapefruit peel extract (GPE) were equal to 117.3 ± 0.3 µg of gallic acid/mg and 39.30 ± 0.1 µg of quercetin/mg respectively. Caspian white fish (n = 180, 4 ± 0.9 g body weight) was fed with supplemented diets, including 0, 6.25, 12.5 and 25 mg of GPE/kg for 60 days at 25 ± 1°C. The growth performance was markedly improved in fish fed with 25 mg/kg of GPE compared to others (p < 0.05). Moreover, fish fed with 25 mg/kg of GPE showed a significant increase in red blood cell (2.65·106 cell/mm3), white blood cell (17.75·103 cell/mm3), packed cell volume (48%) and haemoglobin concentrations (8.75 g/dl) compared to the control (p < 0.05). However, the highest alanine aminotransferase (140 U/L), alanine transaminase (14.5 U/L), and alkaline phosphatase (18.5 U/L) were observed in control group. Morphological analysis of intestine revealed the highest amount of villus width (8.4 µM), height (32.86 µM) and surface area (342.7 µM2) in fish fed with 25 mg/kg of GPE (p < 0.05). In conclusion, supplementing feed with GPE at 25 g/kg can improve growth performance and haemato‐biochemical parameters of Caspian white fish fry.

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“…It has neither lethal effect on fish nor impairs nitrification in biofilters at low dosages (1 mg L -1 ) (Pedersen et al, 2009). In Europe, PAA has been approved for treating pathogens in aquaculture (Straus et al, 2012;Hushangi and Shekarabi, 2018). However, PAA has shown contradictory results: in some cases it prevents some troublesome parasites, while in others it has remained ineffective (Pedersen and Henriksen, 2011;Pedersen et al, 2013).…”

Section: A C C E P T E D Mmentioning

confidence: 99%

Effect of peracetic acid on levels of geosmin, 2-methylisoborneol, and their potential producers in a recirculating aquaculture system for rearing rainbow trout (Oncorhynchus mykiss)

Lindholm‐Lehto

Suurnäkki

Pulkkinen

et al. 2019

Aquacultural Engineering

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In recirculating aquaculture systems (RAS)s, off-flavors and odors, mainly caused by geosmin (GSM) and 2-methylisoborneol (MIB), can accumulate in the flesh of fish from RAS water, reducing the profitability of production. In this study, peracetic acid (PAA) was applied in three application intervals to pump sumps of rainbow trout (Oncorhynchus mykiss) reared in RAS. Using a real-time polymerase chain reaction (qPCR), the potential off-flavor producers were quantified using geoA and MIB synthase genes. Streptomyces was identified as the major GSM producer, and biofilters showed the highest number of potential off-flavor producers. Concentrations of GSM and MIB were analyzed in the circulating water and in the lateral part of the fish fillet. In water, concentrations up to 51 ng L -1 (GSM) and 60.3 ng L -1 (MIB) were found, while in the fillet, these were up to 9.8 ng g -1 (GSM) and 10.2 ng g -1 (MIB), decreasing with increasing number of PAA applications. PAA applications reduced the levels of off-flavor compounds, although this was insufficient to fully prevent the accumulation of GSM and MIB.

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Effect of a Peracetic Acid-Based Disinfectant on Growth, Hematology and Histology of Juvenile Rainbow Trout (Oncorhynchus mykiss)

Cited by 16 publications

References 20 publications

Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid

Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid

Determination of grapefruit (Citrus paradisi) peel extract bio‐active substances and its application in Caspian white fish (Rutilus frisii kutum) diet: Growth, haemato‐biochemical parameters and intestinal morphology

Effect of peracetic acid on levels of geosmin, 2-methylisoborneol, and their potential producers in a recirculating aquaculture system for rearing rainbow trout (Oncorhynchus mykiss)

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