Effect of a novel antimicrobial peptide chrysophsin-1 on oral pathogens and Streptococcus mutans biofilms

Wang, Wei; Tao, Rui; Tong, Zhongchun; Ding, Yue; Kuang, Rongze; Zhai, Shafei; Li, Jun; Ni, Longxing

doi:10.1016/j.peptides.2012.01.006

Cited by 79 publications

(60 citation statements)

References 32 publications

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“…In the present study SM and LB were tested as many studies have confirmed SM and LB as the sole causative microbiological agents and their presences in the dental structure cariogenic biofilm are indicator of dental caries (19) .…”

Section: Discussionmentioning

confidence: 93%

Evaluation of the Effect of Dairy Products on Streptococci and Lactobacilli in Saliva on Group of Egyptian Children

2016

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Objective: to evaluate the effect of dairy products on streptococcus mutans (SM) and lactobacilli (LB) in saliva on group of Egyptian children. Material and Methods: One hundred and twenty Egyptian children from both sexes were included in this study, their age ranged from 4-12 years old. These children were equally divided into 3 groups, each group consisted of 40 children. First group (group A) was instructed to drink plain cow's milk, second group (group B) was instructed to drink milk plus sugar and third group (group C) was instructed to drink milk plus honey. Three saliva samples were collected from each child, one before drinking the beverage (S 1 ) then immediately after drinking (S 2 ) and finally one hour after drinking the beverage (S 3 ) by spitting in a sterile plastic container, then submitted to the Culture and Sensitivity Unit at The Regional center for Mycology and Biotechnology at Al-Azhar University to evaluate the salivary concentration on SM and LB for each subject. Results: The use of plain milk resulted in decrease in the mean value of SM immediately after drinking then subsequently showed a slight increase after one hour. And LB mean value increased immediately after beverage, then subsequently showed a significant increase after one hour. The use of milk plus sugar resulted in significant increase in mean value of SM immediately and one hour after beverage. And the mean value of LB significantly increased immediately and one hour after beverage. The use of milk plus honey resulted in significant decrease in the mean value of SM one hour after beverage .And the mean value of LB significantly decreased immediately and one hour after beverage. Conclusion: the results revealed that, Milk and dairy products have a low cariogenic potential. Adding sugar makes whole milk as cariogenic as a sucrose solution. Natural honey had an antibacterial activity on SM and LB bacteria.Codex : 05/1601 dentaljournal.forgirls@yahoo.com

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Section: Discussionmentioning

confidence: 93%

Evaluation of the Effect of Dairy Products on Streptococci and Lactobacilli in Saliva on Group of Egyptian Children

2016

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“…Other periodontal pathogens were included in the study to evaluate the specificity of the antagonistic substance(s). Even when metronidazole, the drug of choice in periodontal treatment, was employed, Streptococcus mutans and microaerophilic bacteria have been reported to increase their numbers after drug treatment (Wang et al 2012). Positively charged residues on the polar face of the AMP structure allow molecules to target bacteria through electrostatic attraction to their negatively charged phospholipids, and this permits the nonpolar face of the peptide to become inserted into the microbial membrane to modify permeability and the loss of barrier function (Fox et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Many of these species are considered commensal, and some are believed to have a positive effect on our oral health, while others are considered pathogenic and have been implicated in dental caries and periodontitis (Wang et al 2012). Periodontitis is an inflammatory disorder and is initiated by an accumulation of predominantly anaerobic Gram-negative bacteria in subgingival sites.…”

Section: Introductionmentioning

confidence: 99%

Novel antimicrobial peptide specifically active against Porphyromonas gingivalis

et al. 2015

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Porphyromonas gingivalis, the major etiologic agent of chronic periodontitis, produces a broad spectrum of virulence factors, including outer membrane vesicles, lipopolysaccharides, hemolysins and proteinases. Antimicrobial peptides (AMPs) including bacteriocins have been found to inhibit the growth of P. gingivalis; however, these peptides are relatively large molecules. Hence, it is difficult to synthesize them by a scale-up production. Therefore, this study aimed to synthesize a shorter AMP that was still active against P. gingivalis. A peptide that contained three cationic amino acids (Arg, His and Lys), two anionic amino acids (Glu and Asp), hydrophobic amino acids residues (Leu, Ile, Val, Ala and Pro) and hydrophilic residues (Ser and Gly) was obtained and named Pep-7. Its bioactivity and stability were tested after various treatments. The mechanism of action of Pep-7 and its toxicity to human red blood cells were investigated. The Pep-7 inhibited two pathogenic P. gingivalis ATCC 33277 and P. gingivalis ATCC 53978 (wp50) strains at a minimum bactericidal concentration (MBC) of 1.7 µM, but was ineffective against other oral microorganisms (P. intermedia, Tannerella forsythensis, Streptococcus salivarius and Streptococcus sanguinis). From transmission electron microscopy studies, Pep-7 caused pore formation at the poles of the cytoplasmic membranes of P. gingivalis. A concentration of Pep-7 at four times that of its MBC induced some hemolysis but only at 0.3%. The Pep-7 was heat stable under pressure (autoclave at 110 and 121 °C) and possessed activity over a pH range of 6.8-8.5. It was not toxic to periodontal cells over a range of 70.8-4.4 μM and did not induce toxic pro-inflammatory cytokines. The Pep-7 showed selective activity against Porphyromonas sp. by altering the permeability barriers of P. gingivalis. The Pep-7 was not mutagenic in vitro. This work highlighted the potential for the use of this synthetic Pep-7 against P. gingivalis.

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“…Human gingival fibroblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) (Life Technologies, Carlsbad, CA, USA) supplemented with 10% newborn calf serum (Life Technologies) and 1% penicillin-streptomycin (Life Technologies) as previously described [26]. Passage 6 cells were used for the following studies.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

“…Cells that were exposed to medium alone served as controls. The cell viability and levels of extracellular lactate dehydrogenase (LDH) were evaluated as we did previously [26].…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Antimicrobial and anti-biofilm effect of Bac8c on major bacteria associated with dental caries and Streptococcus mutans biofilms

et al. 2014

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Effect of a novel antimicrobial peptide chrysophsin-1 on oral pathogens and Streptococcus mutans biofilms

Cited by 79 publications

References 32 publications

Evaluation of the Effect of Dairy Products on Streptococci and Lactobacilli in Saliva on Group of Egyptian Children

Evaluation of the Effect of Dairy Products on Streptococci and Lactobacilli in Saliva on Group of Egyptian Children

Novel antimicrobial peptide specifically active against Porphyromonas gingivalis

Antimicrobial and anti-biofilm effect of Bac8c on major bacteria associated with dental caries and Streptococcus mutans biofilms

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