Effect of a multicarbohydrase supplementation to diets varying in metabolisable energy level on the performance, carcase traits, caecal microbiota, intestinal morphology, and nutrient digestibility in broiler chickens

Abstract

This trial was conducted to investigate the effects of multi-carbohydrase supplementation on the performance, carcase traits, intestinal histomorphology, caecal microbiota and nutrient digestibility of broiler chickens fed diets with varying energy levels. A total of 400-day-old male chicks were allocated into eight dietary treatments in a 4 Â 2 factorial arrangement with 5 replicates per treatment. There were 4 dietary energy levels (the standard breed recommendation and reductions of 50, 100 and 150 kcal/kg from the standard) with or without multi-carbohydrase supplementation. Reducing the energy level by 50 kcal/kg did not significantly impact the measured parameters compared to the standard recommendation while it is reducing by 100 and 150 kcal/kg resulted in significant decreases in the body weight (BW) by levels up to 4.41% and a significant increase in the feed conversion ratio (FCR) by levels up to 4.67%. Enzyme supplementation significantly improved the BW and FCR by 3.24% and 2.95%, respectively. Dietary energy lowered by 100-150 kcal/kg resulted in a significant decrease in the dressing (up to 2.42%), breast yield (up to 2.82%), fat pads (up to 8.72%), liver (up to 6.30%) percentages and coliform count (up to 28.5%). The digestibility of dry matter, crude protein, ether extract and gross energy was increased due to enzyme supplementation by 4.15, 3.50, 3.35 and 3.72%, respectively. In conclusion, broiler performance and carcase traits can be negatively impacted if the energy density is reduced by 100-150 kcal/kg diet. Enzyme supplementation can improve the performance and nutrient digestibility regardless of the dietary energy level. HIGHLIGHTSThe metabolisable energy level can be reduced by 50 kcal/kg diets compared to the breed recommendation. Decreasing the energy level by 100 or 150 kcal/kg diet compared to breed recommendations resulted in a negative impact on the performance. Multicarbohydrase blend supplementation resulted in significant improvements in the performance and nutrient digestibility.

Effects of an emulsifier blend supplementation on growth performance, nutrient digestibility, intestinal morphology, and muscle fatty acid profile of broiler chickens fed with different levels of energy and protein

“…According to the results of the current study, dietary ME content had no effect on the histomorphological characteristics of the duodenal and jejunal mucosa. These findings are consistent with those of previous studies, which reported no significant variation in the gut histomorphological characteristics of broiler chickens when dietary energy was reduced by 100 kcal/kg ( Wickramasuriya et al, 2019 ) or 150 kcal/kg ( Attia et al, 2021 ). However, the current study found that the duodenum and jejunum of broiler chickens fed a low-CP diet showed a significant trend toward a lower VH when compared to the normal-CP group.…”

Energy values evaluation and improvement of soybean meal in broiler chickens through supplemental mutienzyme

“…Furthermore, the DM and GE digestibility of combined soybean meal 1 were significantly decreased compared with other soybean meals. Interestingly, CP digestibility in our study was lower than that in other studies ( Attia et al., 2021 ), but similarly to that obtained by Liu et al. (2021) .…”

“…In addition, intestinal morphology was improved by carbohydrase supplementation mainly due to reduced viscosity of digesta ( Jang et al., 2020 ). Similarly, Attia et al. (2021) also stated that the supplementation of multi-carbohydrase could additionally or synergistically improve gut microbiota and intestinal histomorphology.…”

Different fat-to-fiber ratios by changing wheat inclusion level impact energy metabolism and microbial structure of broilers

“…Dietary energy density profoundly influences microbiota composition and abundance ( Adewole and Akinyemi, 2021 ). Low energy density decreased the amount of nutrients available for bacterial fermentation, thereby reshaping bacterial structure ( Attia et al, 2021 ). In addition, nutrient utilization of microbiota in hindgut is closely related to energy metabolism ( Yamamura et al, 2021 ).…”