Effect of a modified Nd:YAG laser technique on neuroma formation: An experimental study in rat sciatic nerve

Menovsky, Thomas; Beek, Hans van; Weerman, Marius van den Bergh; Overbeeke, J.J. van

doi:10.1002/(sici)1096-9101(1999)25:3<213::aid-lsm4>3.0.co;2-4

Cited by 12 publications

(13 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Morphological analysis is the most commonly used method, usually performed in light, 18,22,24,26,27,29,45,46,32,34,50,36,38,43,44,49,19,20,47,39,49 electron, 10,30,32,50,36,40 fluorescence 19,37,38,40 or confocal laser microscopy. 20,43,44 Data on the level where biopsies of the nerve and the neuroma are taken and analysed are heterogeneous and often missing, preventing appropriate comparison of results: Chim et al 16 Haematoxylin and eosin 16,18e20,36,50,37,40,49 and toluidine blue 10,18,22,32,45,46,24,19,25,27,30,38,43,…”

Section: Evaluation Methods Histologymentioning

confidence: 99%

“…20,43,44 Data on the level where biopsies of the nerve and the neuroma are taken and analysed are heterogeneous and often missing, preventing appropriate comparison of results: Chim et al 16 Haematoxylin and eosin 16,18e20,36,50,37,40,49 and toluidine blue 10,18,22,32,45,46,24,19,25,27,30,38,43,44 are the most commonly used stains; some studies also use Masson's thricome, 10,20,37 which also allows the labelling of the connective tissue. 70 This is sometimes combined with immunolabelling, the most common targets being S100 protein, 16,25e27,43 neurofilament 200 (NF200), 10,25,30,37,38 neuronal growthassociated protein (GAP)-43, 20,24,43,44 macrophages (ED-1), 20,27,28,43 nerve growth factor (NGF) 27 and calcitonin gene-related peptide (CGRP).…”

Section: Evaluation Methods Histologymentioning

confidence: 99%

“…24,28,30 Double staining has also been reported to identify axons of different origin. 36,39 The most studied morphologic parameters are nerve cross-sectional area, 16,24,26,43e47 the ratio of neural to connective tissue, 16,26,51 myelinated axon count, 24,45e47 neuroma cross-sectional area, 45e47 neuroma size, 20,32,36 scar and regenerative nerve thickness, 19,20 axon and myelin fibre diameter and area. 38 In a recent paper, Yan et al 10 proposed the weight ratio of neuroma/normal nerve segment, which allows to normalise the results, making their comparison easier.…”

Section: Evaluation Methods Histologymentioning

confidence: 99%

“…As pointed out by Lindenlaub et al, 69 the gap length influences neuroma formation; Sinis et al 26 also showed that shorter gaps are associated with larger neuromas, and they postulated that it may be due to the influence of growth factors released from the distal stump. Elwakil et al 48 and Menovski et al 32 reported the use of neodymium-doped yttrium aluminium garnet (nd:YAG) laser to reproduce a terminal neuroma. Some authors combined ligation and transection in a single- 21,23,35 or two-stage model.…”

Section: Mechanism Of Injurymentioning

confidence: 99%

“…To date, the most popular model for terminal neuroma continues to be sciatic nerve neurotmesis in the rat, 10,19,20,24,25,27,29,30,32,34,35,38 but recently, a number of alternative models have been developed, which focus on the investigation of specific aspects of peripheral neuromas.…”

Section: Terminal Neuromasmentioning

confidence: 99%

See 4 more Smart Citations

A systematic review of animal models for experimental neuroma

Toia

Giesen

Giovanoli

et al. 2015

Journal of Plastic, Reconstructive & Aesthetic Surgery

View full text Add to dashboard Cite

Section: Evaluation Methods Histologymentioning

confidence: 99%

Section: Evaluation Methods Histologymentioning

confidence: 99%

Section: Evaluation Methods Histologymentioning

confidence: 99%

Section: Mechanism Of Injurymentioning

confidence: 99%

Section: Terminal Neuromasmentioning

confidence: 99%

See 3 more Smart Citations

A systematic review of animal models for experimental neuroma

Toia

Giesen

Giovanoli

et al. 2015

Journal of Plastic, Reconstructive & Aesthetic Surgery

View full text Add to dashboard Cite

Reduction of post‐traumatic neuroma and epineural scar formation in rat sciatic nerve by application of microcrystallic chitosan

et al. 2011

View full text Add to dashboard Cite

Injury of peripheral nerve is associated with the development of post-traumatic neuroma at the end of the proximal stump, often being the origin of neuropathic pain. This type of pain is therapy-resistant and therefore extremely nagging for patients. We examined the influence of the microcrystallic chitosan gel applied to the proximal stump of totally transected sciatic nerve on the neuroma formation and neuropathic pain development in rats. In 14 rats, right sciatic nerve was transected and the distal stump was removed to avoid spontaneous rejoining. In the chitosan (experimental) group (n = 7), the proximal stump was covered with a thin layer of the microcrystallic chitosan gel. In control animals (n = 7), the cut nerve was left unsecured. Autotomy, an animal model of neuropathic pain, was monitored daily for 20 weeks following surgery. Then, the animals were perfused transcardially and the proximal stumps of sciatic nerves were dissected and subjected to histologic evaluation. The presence, size, and characteristics of neuromas as well as extraneural fibrosis were examined. In chitosan group, the incidence and the size of the neuroma were markedly reduced, as compared with the control group; however, there was no difference in autotomy behavior between groups. In addition, extraneural fibrosis was significantly reduced in chitosan group when compared to the control group. The results demonstrate beneficial influence of microcrystallic chitosan applied to the site of nerve transection on the development of post-traumatic neuroma and reduction of extraneural fibrosis, however without reduction of neuropathic pain.

show abstract

Role of Nd:YAG laser for prevention of neuroma formation: an in vivo experimental study

Elwakil

Elkharbotly

2007

Lasers Med Sci

View full text Add to dashboard Cite

Nerve transection is commonly followed by the development of neuroma at the proximal stump. It can be very painful especially at exposed sites. It may arise spontaneously or after mechanical irritation. Neuroma and its high recurrence rate might be resisting problems to treat. Various treatment modalities for neuroma and its recurrence have been proposed, but none has provided satisfactory results. The present study was conducted to evaluate the neodymium:yttrium aluminum garnet (Nd:YAG) laser (1,064 nm) nerve transection technique for prevention of neuroma formation. There were 48 facial nerves out of 24 Rex rabbits divided into two equal groups. The 24 left-sided facial nerves at group A were subjected to Nd:YAG laser for nerve transection, while the 24 right-sided facial nerves at group B were subjected to scalpel nerve transection. The results were grossly and histopathologically evaluated. Grossly, laser-transected nerves showed an infrequent incidence of neuroma formation. Histopathologically, laser-transected nerves showed photothermal degenerative changes of the axons and myelin sheaths with intact perineurium and endoneurium. No Schwann cell hyperactivity could also be elicited among laser-transected nerves. Nd:YAG laser was found to be an effective tool that could be applied, whenever it is possible, for division of major nerves to prevent the formation of the subsequent stump neuroma. Moreover, this technique should be considered during treatment of well-established neuroma to prevent the challenging reported high incidence of recurrence.

show abstract

Effect of a modified Nd:YAG laser technique on neuroma formation: An experimental study in rat sciatic nerve

Cited by 12 publications

References 17 publications

A systematic review of animal models for experimental neuroma

A systematic review of animal models for experimental neuroma

Reduction of post‐traumatic neuroma and epineural scar formation in rat sciatic nerve by application of microcrystallic chitosan

Role of Nd:YAG laser for prevention of neuroma formation: an in vivo experimental study

Contact Info

Product

Resources

About