Effect of a lytic bacteriophage on rabbits experimentally infected with pathogenic Escherichia coli

Abstract

Pathogenic Escherichia coli (E. coli ) is severely threatening the rabbit industry in China, and the concern over antibiotic-resistant bacteria has given rise to an urgent need for antibiotic alternatives. In this study, a member (ZRP1) of the Myoviridae family was isolated from rabbit faeces using a strain of rabbit atypical enteropathogenic E. coli (ZR1) as host. The one-step growth curve indicated that the latent period was around 25 to 30 min and the burst size was 144±31 plaque-forming unit/cell. The rate of phage-resistant mutation was 7×10 -5 ±4×10-5 . When the bacteriophage input at the multiplicity of infection (MOI) was 0.1, 1 or 10, the growth of host E. coli in broth was inhibited for 5 h. A single intravenous injection of ZRP1 at MOI 0.1, 1 or 10 significantly prolonged the survival time of rabbits which simultaneously received a lethal dose of ZR1.

Characterization and Genome Study of Novel Lytic Bacteriophages against Prevailing Saprophytic Bacterial Microflora of Minimally Processed Plant-Based Food Products

“…Changes in the onset of the logarithmic growth phase in phage-infected cultures were reported by Zhao et al [69]. The authors showed that, regardless of the multiplicity of infection, the bacterial cultures treated with phages began the log-phase significantly later compared to the control.…”

“…Depending on phage type, 2% phosphotungstic acid solution neutralized to pH 7.2 with 1 M potassium hydroxide solution, 2% uranyl acetate solution, or 0.5% iron(II) chloride solution were used for contrasting. Samples were dried for 12 h at ambient temperature under sterile conditions (according to Ackermann [135], Amarillas et al [78], Mahmoud et al [70] procedures with modification), and visualized under JEOL JEM-1220 transmission microscope at 100,000-200,000× magnification, at a voltage of 80 kV [69]. The image background was removed in PhotoScape 3.7 software (MOII TECH, Seoul, Korea).…”

The use of bacteriophages against saprophytic mesophilic bacteria in minimally processed food [pdf]

“…Changes in the onset of the logarithmic growth phase in phage-infected cultures were investigated by Zhao et al (2017). They demonstrated that regardless of the multiplicity of infection, the bacterial cultures treated with phages began the log-phase significantly later than the control cultures.…”

Wykorzystanie fagów litycznych do ograniczania liczby pałeczek Salmonella w roślinnej matrycy żywnościowej

“…Zmiany szybkości rozpoczęcia fazy logarytmicznego wzrostu w hodowlach zainfekowanych fagami badali Zhao i wsp. [35]. Wykazali, że, niezależnie od zastosowanego współczynnika infekcji, hodowle bakteryjne traktowane fagami istotnie później rozpoczynały log-fazę w porównaniu z hodowlami kontrolnymi.…”