Effect of a leucine-enriched essential amino acids mixture on muscle recovery

Matsui, Yasuji; Takayanagi, Shoji; Ohira, T.; Watanabe, Masahiro; Murano, Hiroki; Furuhata, Yasufumi; Miyakawa, Shumpei

doi:10.1589/jpts.31.95

Cited by 8 publications

(5 citation statements)

References 43 publications

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“…Although both exercise and ISPG administration lowered the serum ammonia to relief the peripheral and central fatigue, we did not find a combination benefit of lowering ammonia in the ISPG + RT group. Many studies reported that strenuous exercise cause muscle damage, and creatine kinase and myoglobin in the muscle are released into the blood, thus the level of serum creatine kinase and myoglobin are considered as the reliable biomarkers of muscle damage [36,37]. In our study, we found that the ISPG + RT group had the least level of CK, although not a significant difference.…”

Section: Discussioncontrasting

confidence: 37%

Ergogenic Effects of Green Tea Combined with Isolated Soy Protein on Increasing Muscle Mass and Exercise Performance in Resistance-Trained Mice

et al. 2021

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It is well known that supplementation with high protein after exercise can effectively promote muscle synthesis and repair, while green tea is rich in catechins that have antioxidant effects. We aimed to explore the effects of green tea combined with isolated soy protein on increase muscle mass in resistance-trained mice. A total of 32 male ICR mice (8-weeks old) were divided into four groups (n = 8/group), sedentary control group (SC), isolated soy protein with green tea group (ISPG), resistance training group (RT), isolated soy protein and green tea combine with resistance training group (ISPG + RT). All mice received control or ISPG by oral gavage for four consecutive weeks. Forelimb grip and exhaustive swimming time were used for exercise performance evaluation. In biochemical profile, we analyzed lactate, ammonia, blood urea nitrogen (BUN), and glucose and muscle damage index creatine kinase (CK) after exercise as biochemical parameters of exercise fatigue. The grip strength, muscular endurance, and exhaustive swimming time of the ISPG + RT group were significantly increased than other groups (p < 0.05), and also significantly decreased in serum lactate and ammonia levels (p < 0.05, respectively). The ISP + RT group was not only increased in quadriceps weight, (p < 0.05) but also decreased EFP (p < 0.05). We recommend using a 4-week supplementation with ISPG, combined with RT, to increase muscle mass, exercise performance, glycogen storage, and reduce fatigue biochemical parameters after exercise. The benefits of long-term supplementation or application to human supplementation can be further explored in the future.

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Section: Discussioncontrasting

confidence: 37%

Ergogenic Effects of Green Tea Combined with Isolated Soy Protein on Increasing Muscle Mass and Exercise Performance in Resistance-Trained Mice

et al. 2021

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“…In studies involving eccentric knee extension exercise in untrained males, supplementation with branched-chain amino acids (BCAAs) reduced muscle soreness [30], whereas whey protein supplementation [31] preserved muscle force. Furthermore, improved plasma CK levels were observed with LEAA supplementation following an acute bout of repeated elbow flexion and extension in untrained males [32]. However, similar to 'direct' measures of muscle damage, no study to our knowledge has systematically examined the potential relationship between MyoPS and these common indirect (e.g., soreness) or performance (e.g., strength) markers of muscle damage in response to nutritional intervention, which has been highlighted previously as a limitation [27].…”

Section: Introductionmentioning

confidence: 88%

“…In addition, our interest in concurrently measuring both muscle performance and MyoPS without either objectives influencing the other dictated that we perform a bilateral exercise stimulus, which rendered the unilateral exercise crossover model untenable. Although within-participant study designs may be statistically more robust and limit interindividual physiological and/or perceptual variability, parallel group designs are far more prevalent in studies investigating the impact of amino acid-based interventions on exercise-induced muscle damage given the challenges with the repeated bout effect [12,[29][30][31][32]63,64]. Therefore, although it is possible that we were able to effectively employ a within-participant cross-over design, the small to moderate effect sizes in favour of LEAAs for some secondary outcomes (e.g., muscle strength and soreness) could have reached statistical significance.…”

Section: Effect Of Leaas On Muscle Damagementioning

confidence: 99%

Leucine-Enriched Essential Amino Acids Improve Recovery from Post-Exercise Muscle Damage Independent of Increases in Integrated Myofibrillar Protein Synthesis in Young Men

et al. 2020

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Background: Leucine-enriched essential amino acids (LEAAs) acutely enhance post-exercise myofibrillar protein synthesis (MyoPS), which has been suggested to be important for muscle repair and recovery. However, the ability of LEAAs to concurrently enhance MyoPS and muscle damage recovery in free-living humans has not been studied. Methods: In a randomized, double-blind, placebo-controlled, parallel-group design, twenty recreationally active males consuming a controlled diet (1.2 g/kg/d of protein) were supplemented thrice daily with 4 g of LEAAs (containing 1.6 g leucine) or isocaloric placebo for four days following an acute bout of lower-body resistance exercise (RE). MyoPS at rest and integrated over 96 h of recovery was measured by D2O. Isometric and isokinetic torque, muscle soreness, Z-band streaming, muscle heat shock protein (HSP) 25 and 72, plasma creatine kinase (CK), and plasma interleukin-6 (IL-6) were measured over 96 h post-RE to assess various direct and indirect markers of muscle damage. Results: Integrated MyoPS increased ~72% over 96 h after RE (p < 0.05), with no differences between groups (p = 0.98). Isometric, isokinetic, and total peak torque decreased ~21% by 48 h after RE (p < 0.05), whereas total peak torque was ~10% greater overall during recovery in LEAAs compared to placebo (p < 0.05). There were moderate to large effects for peak torque in favour of LEAAs. Muscle soreness increased during recovery with no statistical differences between groups but small to moderate effects in favour of LEAAs that correlated with changes in peak torque. Plasma CK, plasma IL-6, and muscle HSP25 increased after RE (p < 0.05) but were not significantly different between groups (p ≥ 0.13). Consistent with a trend toward attenuated Z-band streaming in LEAAs (p = 0.07), muscle HSP72 expression was lower (p < 0.05) during recovery in LEAAs compared with placebo. There were no correlations between MyoPS and any measures of muscle damage (p ≥ 0.37). Conclusion: Collectively, our data suggest that LEAAs moderately attenuated muscle damage without concomitant increases in integrated MyoPS in the days following an acute bout of resistance exercise in free-living recreationally active men.

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“…An elevation in blood CK levels typically indicates muscle cell damage and leakage of CK into the bloodstream [34]. Strenuous exercise is commonly associated with muscle damage and an increase in blood CK activity [35]. Notably, the CK activity observed in the CS group was significantly lower than that in the glucose control group (Figure 7D).…”

Section: Serum Fatigue Indexes In Micementioning

confidence: 96%

The Synergistic Effect of Compound Sugar with Different Glycemic Indices Combined with Creatine on Exercise-Related Fatigue in Mice

Liao,

Zhu,

et al. 2024

Foods

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In this study, a compound sugar (CS) with different glycemic index sugars was formulated via hydrolysis characteristics and postprandial glycemic response, and the impact of CS and creatine emulsion on exercise-related fatigue in mice was investigated. Thirty-five C57BL/6 mice were randomly divided into five groups to supply different emulsions for 4 weeks: initial emulsion (Con), glucose emulsion (62 mg/10 g MW glucose; Glu), CS emulsion (62 mg/10 g MW compound sugar; CS), creatine emulsion (6 mg/10 g MW creatine; Cr), and CS and creatine emulsion (62 mg/10 g MW compound sugar, 6 mg/10 g MW creatine, CS-Cr). Then, the exhaustion time of weight-bearing swimming and forelimb grip strength were measured to evaluate the exercise capacity of mice, and some fatigue-related biochemical indexes of blood were determined. The results demonstrated that the ingestion of CS significantly reduced the peak of postprandial blood glucose levels and prolonged the energy supply of mice compared to ingesting an equal amount of glucose. Mouse exhaustion time was 1.22-fold longer in the CS group than in the glucose group. Additionally, the supplementation of CS increased the liver glycogen content and total antioxidant capacity of mice. Moreover, the combined supplementation of CS and creatine increased relative forelimb grip strength and decreased blood creatine kinase activity. The findings suggested that the intake of CS could enhance exercise capacity, and the combined supplementation of CS and creatine has a synergistic effect in improving performance.

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Effect of a leucine-enriched essential amino acids mixture on muscle recovery

Cited by 8 publications

References 43 publications

Ergogenic Effects of Green Tea Combined with Isolated Soy Protein on Increasing Muscle Mass and Exercise Performance in Resistance-Trained Mice

Ergogenic Effects of Green Tea Combined with Isolated Soy Protein on Increasing Muscle Mass and Exercise Performance in Resistance-Trained Mice

Leucine-Enriched Essential Amino Acids Improve Recovery from Post-Exercise Muscle Damage Independent of Increases in Integrated Myofibrillar Protein Synthesis in Young Men

The Synergistic Effect of Compound Sugar with Different Glycemic Indices Combined with Creatine on Exercise-Related Fatigue in Mice

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