Effect of a interleukin-I like factor (mononuclear cell factor) on proteoglycan synthesis in cultured human articular chondrocytes

Bocquet, Jean; Daireaux, M.; Langris, Monique; Jouis, V.; Pujol, Jean-Pierre; Béliard, R; Loyau, G

doi:10.1016/s0006-291x(86)80454-5

Cited by 26 publications

(12 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, IL-1 increased DNA synthesis or proteoglycan synthesis in other culture systems (45,46). On the other hand, IL-l consistently suppressed ALPase activity and mineralization in various culture systems with different batches ofrabbit and porcine chondrocytes (data not shown).…”

Section: Discussionmentioning

confidence: 87%

Effects of interleukin-1 on syntheses of alkaline phosphatase, type X collagen, and 1,25-dihydroxyvitamin D3 receptor, and matrix calcification in rabbit chondrocyte cultures.

Kato¹,

Nakashima²,

Iwamoto³

et al. 1993

J. Clin. Invest.

View full text Add to dashboard Cite

The effect of IL-1 on expression of the mineralization-related phenotype by chondrocytes was examined. In cultures of rabbit growth plate chondrocytes, IL-1 beta at 0.1 ng/ml caused 95% decreases in alkaline phosphatase activity, alkaline phosphatase mRNA levels, the incorporation of 45Ca into insoluble material, and the calcium content during the hypertrophic stage. These effects of IL-1 beta were dose-dependent and were observed in 24-48 h. Furthermore, IL-1 beta suppressed increase in cell size and the syntheses of 1,25-dihydroxyvitamin D3 receptor and type X collagen, other markers of hypertrophy, but had little effect on the synthesis of total protein including type II collagen. The inhibition of calcification was observed only when chondrocytes were exposed to IL-1 before the onset of calcification: IL-1 treatment from the mineralization stage had a marginal effect on 45Ca incorporation into insoluble material. These results suggest that IL-1 inhibits chondrocyte hypertrophy and the onset of calcification in ossifying cartilage. (J. Clin. Invest. 1993. 92:2323-2330

show abstract

Section: Discussionmentioning

confidence: 87%

Effects of interleukin-1 on syntheses of alkaline phosphatase, type X collagen, and 1,25-dihydroxyvitamin D3 receptor, and matrix calcification in rabbit chondrocyte cultures.

Kato¹,

Nakashima²,

Iwamoto³

et al. 1993

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…(17) The present study investigated the effects of strontium ranelate on stromelysin and PG synthesis in human chondrocytes cultured with or without IL-1␤ or IGF-I. In chondrocyte culture, IL-1␤ reproduces the decoupling effect leading to a decrease in PG and an increase in stromelysin synthesis, (4,18) whereas IGF-I stimulates PG production.…”

Section: Introductionmentioning

confidence: 95%

Strontium Ranelate Increases Cartilage Matrix Formation

Henrotin

Labasse

Zheng

et al. 2001

Journal of Bone and Mineral Research

113

View full text Add to dashboard Cite

Based on previous studies showing that strontium ranelate (S12911) modulates bone loss in osteoporosis, it could be hypothesized that this drug also is effective on cartilage degradation in osteoarthritis (OA). This was investigated in vitro on normal and OA human chondrocytes treated or not treated with interleukin-1␤ (IL-1␤). This model mimics, in vitro, the imbalance between chondroformation and chondroresorption processes observed in vivo in OA cartilage. Chondrocytes were isolated from cartilage by enzymatic digestion and cultured for 24 -72 h with 10 ؊4 -10 ؊3 M strontium ranelate, 10 ؊3 M calcium ranelate, or 2 ⅐ 10 ؊3 M SrCl 2 with or without IL-1␤ or insulin-like growth factor I (IGF-I). Stromelysin activity and stromelysin quantitation were assayed by spectrofluorometry and enzyme amplified sensitivity immunoassay (EASIA), respectively. Proteoglycans (PG) were quantified using a radioimmunoassay. Newly synthesized glycosaminoglycans (GAGs) were quantified by labeled sulfate (Na 2 35 SO 4 ) incorporation. This method allowed the PG size after exclusion chromatography to be determined. Strontium ranelate, calcium ranelate, and SrCl 2 did not modify stromelysin synthesis even in the presence of IL-1␤. Calcium ranelate induced stromelysin activation whereas strontium compounds were ineffective. Strontium ranelate and SrCl 2 both strongly stimulated PG production suggesting an ionic effect of strontium independent of the organic moiety.

show abstract

“…In contrast, indomethacin was shown to suppress glycosaminoglycan synthesis [42]. Besides, it is worth noting that in chondrocyte cultures aspirin has no effect on proteoglycan production [43] but on the other hand, indomethacin inhibits this synthesis [44,45]. As chondrocyte monolayer cultures were proved to be relevant for studying the action of many non steroidal anti-inflammatory drugs on proteoglycan synthesis, this model was thought to be a good approach for estimating the direct action of D-Pen (which is accepted as a disease modifying antirheumatic drug) on cartilage proteoglycans.…”

Section: Discussionmentioning

confidence: 99%

“…According to calibration curves recently published [57], the molecular weight of these proteoglycans can be estimated aproximatively 300 kD. Monomeric proteoglycans extracted from articular cartilage [58] or chondrocyte cultures [45] are generally eluted with an approximate Kav= 0.3 (Sepharose CL-2B) corresponding to a molecular weight of about 1 to 2.5 • 10 6 daltons [59]. Nevertheless, such proteoglycans with a low molecular weight have already been identified in chondrocyte cultures [60][61][62].…”

Section: Discussionmentioning

confidence: 99%

Proteoglycan biosynthesis by rabbit articular chondrocytes treated withd-penicillamine

et al. 1988

Self Cite

View full text Add to dashboard Cite

Rabbit articular chondrocytes in confluent monolayer cultures were treated with D-Penicillamine (D-Pen) during 3 or 5 days. The [35S]-sulfate incorporation in neosynthesized proteoglycans was not modified by D-Pen doses ranging from 50 to 800 micrograms/ml. After treatment during 5 days with D-Pen concentrations of 50 or 400 micrograms/ml, the chemical characteristics of proteoglycans from medium and cell-layer were determined. The aggregation capacity of proteoglycans from medium, the monomer molecular size, the glycosaminoglycan chain length and the relative rates of the different glycosaminoglycans (chondroitins, chondroitin 6-sulfate, chondroitin 4-sulfate, hyaluronic acid) remained unchanged. These results suggest that D-Pen does not alter some of the cartilage mechanical properties due to the presence of proteoglycans.

show abstract

Effect of a interleukin-I like factor (mononuclear cell factor) on proteoglycan synthesis in cultured human articular chondrocytes

Cited by 26 publications

References 42 publications

Effects of interleukin-1 on syntheses of alkaline phosphatase, type X collagen, and 1,25-dihydroxyvitamin D3 receptor, and matrix calcification in rabbit chondrocyte cultures.

Effects of interleukin-1 on syntheses of alkaline phosphatase, type X collagen, and 1,25-dihydroxyvitamin D3 receptor, and matrix calcification in rabbit chondrocyte cultures.

Strontium Ranelate Increases Cartilage Matrix Formation

Proteoglycan biosynthesis by rabbit articular chondrocytes treated withd-penicillamine

Contact Info

Product

Resources

About