Effect of a high linoleic acid diet on Δ9-desaturase activity, lipogenesis and lipid composition of pig subcutaneous adipose tissue

Abstract

-The effects of three diets were compared: a high linoleic acid diet (diet M containing 4 % maize oil), diet T containing 4 % beef tallow, and C, a conventional control diet, on A9-desaturase activity and lipogenesis in pig subcutaneous adipose tissue. Diet M increased lipogenesis (estimated from the activities of acetyl-CoA-carboxylase, malic enzyme and glucose-6-phosphate dehydrogenase), and decreased A9-desaturase activity, in comparison to the other diets. Linoleic acid content was higher in the pigs fed diet M than in the other pigs (amounting to 26 % of total tissue fatty acids versus 15 %, respectively). The lower monounsaturated fatty acid content in adipose tissue of pigs fed diet M compared to pigs fed other diets could be associated with the lower A9-desaturase activity and the lower oleic acid content of diet M. The present study suggests that A9-desaturase could be involved in the regulation of monounsaturated fatty acid con-

Dietary fat source affects metabolism of fatty acids in pigs as evaluated by altered expression of lipogenic genes in liver and adipose tissues

“…In contrast to the abundance of liver SREBP-1 mRNA, adipose tissue showed no differences between treatments, suggesting that fat synthesis in pig adipose tissue could be regulated in a different manner than in the liver. This is consistent with findings by Kouba and Mourot (1998) and Waterman et al (1975). Pigs fed the T diet, which had a high content of SFA, showed the lowest ACACA and FASN mRNA abundance and pigs fed the SFO diet, rich in linoleic acid, showed the highest expression of these genes among fat-added diets, suggesting that SFA inhibit the expression of these genes; other unsaturated fats resulted in intermediate values.…”

“…In rodents (Blake and Clarke, 1990;Iritani et al, 1998) diets containing high levels of PUFA result in lower lipogenesis than those fed diets containing high levels of saturated or MUFA and in broilers SFO (a PUFA-rich diet) lowers FA synthesis (Sanz et al, 2000). Different studies with pigs report higher lipogenic rates in animals fed unsaturated FA than SFA (Waterman et al, 1975;Freire et al, 1998;Kouba and Mourot, 1998;Kouba et al, 1999). According to Chilliard (1993) and Azain (2004), in species like rodents and poultry where the liver is the primary site of lipogenesis, unsaturated FA are more inhibitory on FA synthesis than saturated FA.…”

Effect of a linseed diet on lipogenesis, fatty acid composition and stearoyl-CoA-desaturase in rabbits

“…As for lipogenic enzymes, the SCD activity is the highest in perirenal fat, regardless of the diet. The inhibitory effect of PUFA on SCD activity observed in this study in rabbit liver and adipose tissues has already been demonstrated in pig adipose tissue (Kouba and Mourot, 1998;Kouba et al, 2003) and more recently in the muscle of beef cattle (Waters et al, 2009). The lack of effect of linseed diet on muscle enzyme activity in the rabbits observed in this study had also been observed in pig muscle (Kouba et al, 2003).…”

The quality of lipid ingredients for pig diets: effects on performance, carcass and meat traits and adipocyte diameter

“…The results of the meat percentage analysis corroborated the findings of Halas et al (2010) and Duran-Montgé et al (2008), who demonstrated the possibility of supplementing different lipid sources into pig feed without negative consequences for these parameters, but contradicted those of Kouba and Mourot (1998) and Realini et al (2010), who indicated that ingredients with higher linoleic acid levels (plant-based oils compared with pig lard) increase muscle fat deposition.…”